Adult neural precursor cells from the subventricular zone contribute significantly to oligodendrocyte regeneration and remyelination
Open accessAbstract
Parenchymal oligodendrocyte progenitor cells (pOPCs) are considered the principal cell type responsible for oligodendrogenesis and remyelinaton in demyelinating diseases. Recent studies have demonstrated that neural precursor cells (NPCs) from the adult subventricular zone (SVZ) can also generate new oligodendrocytes after demyelination. However, the relative contribution of NPCs versus pOPCs to remyelination is unknown. We used in vivo genetic fate mapping to assess the behavior of each progenitor type within the corpus callosi (CCs) of mice subjected to cuprizone-induced demyelination. Nestin-CreER(T2) and Pdgfra-CreER(T2) transgenic mice were crossed with fluorescent Cre reporter strains to map the fate of NPCs and pOPCs respectively. In cuprizone-challenged mice, substantial numbers of NPCs migrated into the demyelinated CC and contributed to oligodendrogenesis. This capacity was most prominent in rostral regions adjacent to the SVZ where NPC-derived oligodendrocytes significantly outnumbered those generated from pOPCs. Sixty-two percent of all nodes of Ranvier in this region were flanked by at least one paranode generated from an NPC-derived oligodendrocyte. Remarkably, g-ratios (ratio of the axon diameter to the diameter of the axon plus myelin sheath) of myelinated axons in regions subject to significant NPC-derived remyelination were equivalent to those of unchallenged controls, and immunoelectron microscopy revealed that NPC-derived myelin was significantly thicker t…
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Newly formed cells might be much more efficient compared to surviving oligodendrocytes.
“and it has been demonstrated that newly formed cells might be much more efficient compared to surviving oligodendrocytes.”
From BCAS1-positive oligodendrocytes enable efficient cortical remyelination in multiple sclerosis (2025) · cites this paper as [26]
NPC-derived oligodendrocytes are an important source of new oligodendrocytes in regions of the rostral but not the caudal corpus callosum after cuprizone-induced demyelination.
“They showed that NPC-derived oligodendrocytes are an important source of new oligodendrocytes in regions of the rostral but not the caudal corpus callosum [115].”
From How to Use the Cuprizone Model to Study De- and Remyelination (2024) · cites this paper as [115]
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“26,70 Further experimental work, including lineage tracing experiments and an assessment of internode length and g-ratio will be required to address this issue.”
- Authors
- Xing, Y. L., Röth, P. T., Stratton, J. A. S., Chuang, B. H. A., Danne, J., Ellis, S. L., Ng, S. W., Kilpatrick, T. J., & Merson, T. D.
- Affiliations
- Florey Institute of Neuroscience and Mental Health (AU)
- Peter MacCallum Cancer Centre (AU)
- The University of Melbourne (AU)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2014-10-15
- Volume
- 34
- Issue
- 42
- Pages
- 14128-14146
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6705285
- OpenAlex
- W2006897862
- Cited by
- 275
- References
- 9
- Citations by year
- 2026: 9 · 2025: 17 · 2024: 30 · 2023: 21 · 2022: 28 · 2021: 23 · 2020: 34 · 2019: 27 · 2018: 23 · 2017: 27 · 2016: 19 · 2015: 16 · 2014: 1
- FWCI
- 7.31
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Journal of Neuroscience (publishedVersion, cc-by)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Multiple Sclerosis Australia
- Stem Cells Australia
- Medical Research Council
- Australian Research Council
- National Health and Medical Research Council
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- subventricular zone, oligodendrocyte precursor cells, remyelination, oligodendrogenesis, cuprizone-induced demyelination, corpus callosum, demyelinating disease, paranodes, g-ratio, myelin thickness
- MeSH terms
- Age Factors, Animals, Cell Differentiation, Female, Male, Mice, Inbred C57BL, Mice, Transgenic, Myelin Sheath, Nerve Regeneration, Oligodendroglia, Lateral Ventricles, Mice, Rats, Adult Stem Cells, Neural Stem Cells
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