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Firing and Cellular Properties of V2a Interneurons in the Rodent Spinal Cord
Open accessAbstract
Previous studies have shown that a group of ventrally located neurons, designated V2a interneurons, play a key role in maintaining locomotor rhythmicity and in ensuring appropriate left–right alternation during locomotion (Crone et al., 2008, 2009). These V2a interneurons express the transcription factor Chx10. The aim of the present study was to characterize the locomotor-related activity of individual V2a interneurons, their cellular properties, and their detailed anatomical attributes in Chx10-GFP mice. A dorsal horn-removed preparation was developed to allow for visual whole-cell patch recordings from V2a interneurons along the entire lumbar spinal cord while at the same time leaving enough of the spinal cord intact to generate fictive locomotion. During drug-evoked locomotor-like activity, a large proportion of Chx10 cells showed rhythmic firing or membrane potential fluctuations related to either flexor or extensor activity in every lumbar segment. Chx10 cells received predominantly rhythmic excitatory input. Chx10 neurons displayed a wide variety of firing and potential rhythmogenic properties. However, none of these properties was obviously related to the observed rhythmicity during locomotor-like activity. In dual recordings, we found no evidence of Chx10 neuron interconnectivity. Intracellular fills revealed diverse projection patterns with most Chx10 interneurons being local with projections to the central pattern generator and motor neuron regions of the spinal cord and others with long ascending and/or descending branches. These data are compatible with V2a neurons having a role in regulating segmental left–right alternation and ipsilateral motor neuron firing with little effect on rhythm generation.
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“Specific subpopulations of V2a neurons in the brainstem, cervical spinal cord and lumbar spinal cord have been implicated in different aspects of motor control35,41–45.”
From The neurons that restore walking after paralysis (2022) · cites this paper as [43]
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“These neurons originate from a subset of developmentally defined V2a neurons35,41–45, which describe a family of neuronal subpopulations distributed along the neuraxis35,41–45.”
From The neurons that restore walking after paralysis (2022) · cites this paper as [43]
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Cites
Circular Statistics in Biology
Watson, G. S., & Batschelet, E. (1982) · Technometrics · doi:10.2307/1267831 · Cited by 4,573
Dynamics of the Hippocampal Ensemble Code for Space
Wilson, M. A., & McNaughton, B. L. (1993) · Science · doi:10.1126/science.8351520 · Cited by 2,350
A gene expression atlas of the central nervous system based on bacterial artificial chromosomes
Gong, S., Zheng, C., Doughty, M. L., Losos, K., Didkovsky, N., Schambra, U. B., Nowak, N. J., Joyner, A., Leblanc, G., Hatten, M. E., et al. (2003) · Nature · doi:10.1038/nature02033 · Cited by 2,108
Neuronal specification in the spinal cord: inductive signals and transcriptional codes
Jessell, T. M. (2000) · Nature Reviews Genetics · doi:10.1038/35049541 · Cited by 2,089
Statistics of Directional Data
(1972) · Elsevier eBooks · doi:10.1016/c2013-0-07425-7 · Cited by 1,648
Principles of rhythmic motor pattern generation
Marder, E. E., & Calabrese, R. (1996) · Physiological Reviews · doi:10.1152/physrev.1996.76.3.687 · Cited by 1,287
Interneuronal relay in spinal pathways from proprioceptors
Jankowska, E. (1992) · Progress in Neurobiology · doi:10.1016/0301-0082(92)90024-9 · Cited by 931
LOCOMOTOR CIRCUITS IN THE MAMMALIAN SPINAL CORD
Kiehn, O. (2006) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.29.051605.112910 · Cited by 868
Self-citationThe motor infrastructure: from ion channels to neuronal networks
Grillner, S. E. (2003) · Nature reviews. Neuroscience · doi:10.1038/nrn1137 · Cited by 839
Georgopoulos, A. P., Kettner, R., & Schwartz, A. (1988) · Journal of Neuroscience · doi:10.1523/jneurosci.08-08-02928.1988 · Cited by 835
Research works printed but not linked: 2
- Dougherty, 2008, Characterization of excitatory V2a interneurons in the mammalian spinal cord, Soc Neurosci Abstr, 34, 374
- Dougherty, 2009, Rhythmogenic properties and connectivity of a transcriptionally defined subset of ipsilateral excitatory interneurons in mouse spinal cord, Soc Neurosci Abstr, 35, 366
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Metadata
- Authors
- Dougherty, K. J., & Kiehn, O.
- Affiliations
- Karolinska Institutet (SE)
- Corresponding author
- Kiehn, Ole
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2010-01-06
- Volume
- 30
- Issue
- 1
- Pages
- 24-37
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC3842458
- OpenAlex
- W2166837618
- Cited by
- 138
- References
- 105
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 8 · 2023: 6 · 2022: 13 · 2021: 7 · 2020: 10 · 2019: 9 · 2018: 12 · 2017: 7 · 2016: 4 · 2015: 7 · 2014: 5 · 2013: 7 · 2012: 8
- FWCI
- 4.91
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation (0701166)
- European Commission
- Hjärnfonden
- Karolinska Institutet
- National Institutes of Health (5r01ns040795-09)
- Medical Research Council
- National Institute of Neurological Disorders and Stroke (R01 NS040795, R01NS040795-08)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Motor Control and Adaptation
- Physical Sciences › Engineering › Control and Systems Engineering › Robotic Locomotion and Control
- Keywords
- V2a interneurons, Chx10, spinal cord, locomotor rhythm, fictive locomotion, left-right alternation, central pattern generator, neuronal firing patterns, membrane potential fluctuations, interneuron connectivity, axonal projections, lumbar spinal cord
- MeSH terms
- Action Potentials, Animals, Animals, Newborn, Anterior Horn Cells, Interneurons, Mice, Transgenic, Periodicity, Spinal Cord, Transcription Factors, Homeodomain Proteins, Mice
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