The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Functional and Selective RNA Interference in Developing Axons and Growth Cones
Open accessAbstract
Developing axons and growth cones contain “local” mRNAs that are translated in response to various extracellular signaling molecules and have roles in several processes during axonal development, including axonal pathfinding, orientation of axons in chemotactic gradients, and in the regulation of neurotransmitter release. The molecular mechanisms that regulate mRNA translation within axons and growth cones are unknown. Here we show that proteins involved in RNA interference (RNAi), including argonaute-3 and argonaute-4, Dicer, and the fragile X mental retardation protein, are found in developing axons and growth cones. These proteins assemble into functional RNA-induced silencing complexes as transfection of small interfering RNAs selectively into distal axons results in distal axon-specific mRNA knock-down, without reducing transcript levels in proximal axons or associated diffusion of small interfering RNA into proximal axons or cell bodies. RhoA mRNA is localized to axons and growth cones, and intra-axonal translation of RhoA is required for growth cone collapse elicited by Semaphorin 3A (Sema3A), an axonal guidance cue. Selective knock-down of axonal RhoA mRNA abolishes Sema3A-dependent growth cone collapse. Our results demonstrate functional and potent RNAi in axons and identify an approach to spatially regulate mRNA transcripts at a subcellular level in neurons.
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Cites
MicroRNAs Regulate Brain Morphogenesis in Zebrafish
Giraldez, A. J., Cinalli, R. M., Glasner, M. E., Enright, A. J., Thomson, J. M., Baskerville, S., Hammond, S. M., Bartel, D. P., & Schier, A. F. (2005) · Science · doi:10.1126/science.1109020 · Cited by 1,315
A microRNA array reveals extensive regulation of microRNAs during brain development
KRICHEVSKY, A. M., KING, K. S., DONAHUE, C. P., KHRAPKO, K., & KOSIK, K. S. (2003) · RNA · doi:10.1261/rna.5980303 · Cited by 1,040
Fragile X-related protein and VIG associate with the RNA interference machinery
Caudy, A. A., Myers, M., Hannon, G. J., & Hammond, S. M. (2002) · Genes & Development · doi:10.1101/gad.1025202 · Cited by 655
Weiler, I. J., Irwin, S. A., Klintsova, A. Y., Spencer, C. M., Brazelton, A. D., Miyashiro, K., Comery, T. A., Patel, B., Eberwine, J., & Greenough, W. T. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.10.5395 · Cited by 644 · cited 1×
“FMRP has previously been found in dendrites (Weiler et al., 1997), but its localization in developing axons has not been addressed.”
BackgroundA Drosophila fragile X protein interacts with components of RNAi and ribosomal proteins
Ishizuka, A., Siomi, M. C., & Siomi, H. (2002) · Genes & Development · doi:10.1101/gad.1022002 · Cited by 581
From mRNP trafficking to spine dysmorphogenesis: the roots of fragile X syndrome
Bagni, C., & Greenough, W. T. (2005) · Nature Reviews Neuroscience · doi:10.1038/nrn1667 · Cited by 475
Chen, L., Yun, S.-W., Seto, J., Liu, W., & Toth, M. (2003) · Neuroscience · doi:10.1016/s0306-4522(03)00406-8 · Cited by 160
Cited by
LNCcation: lncRNA localization and function
Bridges, M. C., Daulagala, A. C., & Kourtidis, A. (2021) · Journal of Cell Biology · doi:10.1083/jcb.202009045 · Cited by 1,556
Fragile X Syndrome: Loss of Local mRNA Regulation Alters Synaptic Development and Function
Bassell, G. J., & Warren, S. T. (2008) · Neuron · doi:10.1016/j.neuron.2008.10.004 · Cited by 1,051
Xin, H., Li, Y., Liu, Z., Wang, X., Shang, X., Cui, Y., Zhang, Z. G., & Chopp, M. (2013) · Stem Cells · doi:10.1002/stem.1409 · Cited by 720
Neural tissue engineering options for peripheral nerve regeneration
Gu, X., Ding, F., & Williams, D. F. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2014.04.064 · Cited by 630
Guo, S., Perets, N., Betzer, O., Ben-Shaul, S., Sheinin, A., Michaelevski, I., Popovtzer, R., Offen, D., & Levenberg, S. (2019) · ACS Nano · doi:10.1021/acsnano.9b01892 · Cited by 424
Metadata
- Authors
- Hengst, U., Cox, L. J., Macosko, E. Z., & Jaffrey, S. R.
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2006-05-24
- Volume
- 26
- Issue
- 21
- Pages
- 5727-5732
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- OpenAlex
- W1987877227
- Cited by
- 165
- References
- 7
- FWCI
- 5.90
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Hengst, U., Cox, L. J., Macosko, E. Z., & Jaffrey, S. R. (2006). Functional and Selective RNA Interference in Developing Axons and Growth Cones. The Journal of Neuroscience. https://doi.org/10.1523/jneurosci.5229-05.2006
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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