Fragile X-related protein and VIG associate with the RNA interference machinery
Abstract
RNA interference (RNAi) is a flexible gene silencing mechanism that responds to double-stranded RNA by suppressing homologous genes. Here, we report the characterization of RNAi effector complexes (RISCs) that contain small interfering RNAs and microRNAs (miRNAs). We identify two putative RNA-binding proteins, the Drosophila homolog of the fragile X mental retardation protein (FMRP), dFXR, and VIG (Vasa intronic gene), through their association with RISC. FMRP, the product of the human fragile X locus, regulates the expression of numerous mRNAs via an unknown mechanism. The possibility that dFXR, and potentially FMRP, use, at least in part, an RNAi-related mechanism for target recognition suggests a potentially important link between RNAi and human disease.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
FMRP-associated miRNAs 125b and 132 modulate dendritic spine morphology together with FMRP through common mRNA targets.
“Since FMRP is not needed for general RNAi function (Caudy et al., 2002; Hock et al., 2007), these findings support the idea that the FMRP-associated miRNAs 125b and 132 modulate dendritic spine morphology together with FMRP through common mRNA targets.”
From Regulation of Synaptic Structure and Function by FMRP-Associated MicroRNAs miR-125b and miR-132 (2010) · cites this paper as Caudy et al., 2002
The lack of a canonical miRNA/siRNA binding domain in FMRP suggests that miRNAs are indirectly associated with FMRP, possibly through Argonaute proteins.
“The lack of a canonical miRNA/siRNA binding domain in FMRP suggests that miRNAs are indirectly associated with FMRP, possibly through Argonaute proteins which contain a miRNA binding domain and interact with FMRP in an RNA-independent manner (Caudy et al., 2002; Ishizuka et al., 2002).”
From Regulation of Synaptic Structure and Function by FMRP-Associated MicroRNAs miR-125b and miR-132 (2010) · cites this paper as Caudy et al., 2002
FMRP interacts with proteins in RISC and with miRNAs, but FMRP itself is not essential for RNAi-mediated mRNA cleavage.
“FMRP interacts with proteins (e.g. Argonaute and Dicer) in the RNA interference silencing complex (RISC) and with miRNAs, but FMRP itself is not essential for RNAi-mediated mRNA cleavage (Bolduc et al., 2008; Caudy et al., 2002; Cheever and Ceman, 2009; Hock et al., 2007; Ishizuka et al., 2002; Jin et al., 2004b; Okamura et al., 2004; Plante et al., 2006).”
From Regulation of Synaptic Structure and Function by FMRP-Associated MicroRNAs miR-125b and miR-132 (2010) · cites this paper as Caudy et al., 2002
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Eberhart, D. (1996) · Human Molecular Genetics · doi:10.1093/hmg/5.8.1083 · Cited by 390 · see citing works
Flagged first, then by citations
Research works printed but not linked: 5
- Dudley, N.R., Labbe, J.C., and Goldstein, B. 2002. Using RNA interfer- ence to identify genes required for RNA interference. Proc. Natl. Acad. Sci. 99: 4191-4196.
- Hutvágner, G. and Zamore, P.D. 2002. A MicroRNA in a multiple-turn- over RNAi enzyme complex. Science (in press).
- Ketting, R.F., Fischer, S.E., Bernstein, E., Sijen, T., Hannon, G.J., and Plasterk, R.H. 2001. Dicer functions in RNA interference and in syn- thesis of small RNA involved in developmental timing in C. elegans. Genes & Dev. 15: 2654-2659.
- Paddison, P.J., Caudy, A.A., and Hannon, G.J. 2002. Stable suppression of gene expression by RNAi in mammalian cells. Proc. Natl Acad Sci. 99: 1443-1448.
- Williams, R.W. and Rubin, G.M. 2002. ARGONAUTE1 is required for efficient RNA interference in Drosophila embryos. Proc. Natl. Acad. Sci. 99: 6889-6894.
Cited by
Sorted by citations
A Mammalian microRNA Expression Atlas Based on Small RNA Library Sequencing
Landgraf, P., Rusu, M., Sheridan, R., Sewer, A., Iovino, N., Aravin, A., Pfeffer, S., Rice, A., Kamphorst, A. O., Landthaler, M., et al. (2007) · Cell · doi:10.1016/j.cell.2007.04.040 · Cited by 3,626 · see citing works
Processing of primary microRNAs by the Microprocessor complex
Denli, A. M., Tops, B. B. J., Plasterk, R. H. A., Ketting, R. F., & Hannon, G. J. (2004) · Nature · doi:10.1038/nature03049 · Cited by 2,784 · see citing works
Self-citationThe mGluR theory of fragile X mental retardation
Bear, M. F., Huber, K. M., & Warren, S. T. (2004) · Trends in Neurosciences · doi:10.1016/j.tins.2004.04.009 · Cited by 1,580 · see citing works
A microRNA array reveals extensive regulation of microRNAs during brain development
KRICHEVSKY, A. M., KING, K. S., DONAHUE, C. P., KHRAPKO, K., & KOSIK, K. S. (2003) · RNA · doi:10.1261/rna.5980303 · Cited by 1,040 · see citing works
Regulation of Synaptic Structure and Function by FMRP-Associated MicroRNAs miR-125b and miR-132
Edbauer, D., Neilson, J. R., Foster, K. A., Wang, C.-F., Seeburg, D. P., Batterton, M. N., Tada, T., Dolan, B. M., Sharp, P. A., & Sheng, M. (2010) · Neuron · doi:10.1016/j.neuron.2010.01.005 · Cited by 735 · see citing works
Altered brain microRNA biogenesis contributes to phenotypic deficits in a 22q11-deletion mouse model
Stark, K. L., Xu, B., Bagchi, A., Lai, W.-S., Liu, H., Hsu, R., Wan, X., Pavlidis, P., Mills, A. A., Karayiorgou, M., et al. (2008) · Nature Genetics · doi:10.1038/ng.138 · Cited by 648 · see citing works
AU-rich-element-mediated upregulation of translation by FXR1 and Argonaute 2
Vasudevan, S., & Steitz, J. A. (2007) · Cell · doi:10.1016/j.cell.2007.01.038 · Cited by 656 · see citing works
Park, S., Park, J. M., Kim, S., Kim, J.-A., Shepherd, J. D., Smith-Hicks, C. L., Chowdhury, S., Kaufmann, W., Kuhl, D., Ryazanov, A. G., et al. (2008) · Neuron · doi:10.1016/j.neuron.2008.05.023 · Cited by 516 · see citing works
From mRNP trafficking to spine dysmorphogenesis: the roots of fragile X syndrome
Bagni, C., & Greenough, W. T. (2005) · Nature Reviews Neuroscience · doi:10.1038/nrn1667 · Cited by 475 · see citing works
The Pathophysiology of Fragile X (and What It Teaches Us about Synapses)
Bhakar, A. L., Dölen, G., & Bear, M. F. (2012) · Annual Review of Neuroscience · doi:10.1146/annurev-neuro-060909-153138 · Cited by 370 · see citing works
<i>Drosophila</i> PIWI associates with chromatin and interacts directly with HP1a
Brower-Toland, B., Findley, S. D., Jiang, L., Liu, L., Yin, H., Dus, M., Zhou, P., Elgin, S. C. R., & Lin, H. (2007) · Genes & Development · doi:10.1101/gad.1564307 · Cited by 350 · see citing works
Schenck, A., Bardoni, B., Langmann, C., Harden, N., Mandel, J.-L., & Giangrande, A. (2003) · Neuron · doi:10.1016/s0896-6273(03)00354-4 · Cited by 316 · see citing works
Darnell, J. C., Fraser, C. E., Mostovetsky, O., Stefani, G., Jones, T. A., Eddy, S. R., & Darnell, R. B. (2005) · Genes & Development · doi:10.1101/gad.1276805 · Cited by 287 · see citing works
Fragile X mental retardation protein is associated with translating polyribosomes in neuronal cells
Stefani, G., Fraser, C. E., Darnell, J. C., & Darnell, R. B. (2004) · The Journal of Neuroscience · doi:10.1523/jneurosci.2306-04.2004 · Cited by 274 · see citing works
Qin, M., Kang, J., Burlin, T. V., Jiang, C., & Smith, C. B. (2005) · The Journal of Neuroscience · doi:10.1523/jneurosci.0093-05.2005 · Cited by 263 · see citing works
Truesdell, S. S., Mortensen, R. D., Seo, M., Schroeder, J. C., Lee, J. H., LeTonqueze, O., & Vasudevan, S. (2012) · Scientific Reports · doi:10.1038/srep00842 · Cited by 173 · see citing works
Emerging role of microRNAs in major depressive disorder: diagnosis and therapeutic implications
Dwivedi, Y. (2014) · Dialogues in Clinical Neuroscience · doi:10.31887/dcns.2014.16.1/ydwivedi · Cited by 169 · see citing works
A mouse model of the human Fragile X syndrome I304N mutation
Zang, J. B., Nosyreva, E. D., Spencer, C. M., Volk, L. J., Musunuru, K., Zhong, R., Stone, E. F., Yuva-Paylor, L. A., Huber, K. M., Paylor, R., et al. (2009) · PLoS Genetics · doi:10.1371/journal.pgen.1000758 · Cited by 138 · see citing works
de Bartolomeis, A., Iasevoli, F., Tomasetti, C., & Buonaguro, E. F. (2015) · Molecular Neurobiology · doi:10.1007/s12035-014-8962-8 · Cited by 21 · see citing works
- Authors
- Caudy, A. A., Myers, M., Hannon, G. J., & Hammond, S. M.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2002-10-01
- Volume
- 16
- Issue
- 19
- Pages
- 2491-2496
- ISSN
- 0890-9369
- 1549-5477
- Cited by
- 499
- References
- 1
- Language
- en
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
