Oocyte maturation, fertilization and embryo development in vitro and in vivo in the gaur ( Bos gaurus )
Open accessAbstract
A study was conducted to evaluate the potential of rescuing immature oocytes from the ovaries of an endangered wild bovid, the gaur (Bos gaurus). Recovered, immature gaur oocytes (n = 59) placed in culture were evaluated for: (1) nuclear maturation after 22 h of culture, (2) fertilization with either thawed homologous (gaur) or heterologous (Bos taurus) spermatozoa 18 h after insemination and (3) embryo development. Gaur oocytes (n = 6) evaluated by fixation and staining at 22 h had all matured to metaphase II in vitro. Insemination of gaur oocytes in vitro resulted in normal fertilization (defined as the presence of spermatozoa head or two pronuclei) and embryo development to the two- and four-cell stage of 53.6% (15 of 28) and 50.0% (9 of 18), respectively, using homologous spermatozoa. The incidence of normal fertilization of in vitro matured (IVM) gaur oocytes with heterologous spermatozoa was 53.8% (7 of 13). Insemination of domestic cow oocytes in vitro resulted in normal fertilization and embryo development of 41.7% (45 of 108) and 60.0% (12 of 20), respectively, using heterologous spermatozoa. Two of four gaur embryos (50%) developed to the blastocyst stage by day 7. Embryo transfer of these two conspecific gaur blastocysts into two Holstein recipients resulted in one confirmed pregnancy. One live-born calf was delivered by Caesarean section 308 days after embryo transfer. These results demonstrate the potential of combined IVM and IVF for recovering immature germplasm from an endangered species. Specifically, immature gaur ovarian oocytes are capable of in vitro maturation and fertilization with thawed homologous spermatozoa.(ABSTRACT TRUNCATED AT 250 WORDS)
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The resulting embryos from in vitro maturation and fertilization of immature Bos gaurus oocytes were capable of advancing to blastocysts in culture and of producing live-born offspring after embryo transfer.
“Johnston et al. (1994) found that ovarian oocytes of immature Bos gaurus were capable of in vitro maturation and fertilization with thawed homologous spermatozoa and that the resulting embryos were capable of advancing to blastocysts in culture and of producing live-born offspring after embryo transfer.”
From Bos frontalis and Bos gaurus (Artiodactyla: Bovidae) (2018) · cites this paper as Johnston et al. 1994
The embryos developed to the blastocyst stage in 7 days.
“Specifically, the embryos developed to the blastocyst stage in 7 days, and a live-born calf was delivered 308 days after transferring the embryos to Holstein recipients.”
From Bos frontalis and Bos gaurus (Artiodactyla: Bovidae) (2018) · cites this paper as Johnston et al. 1994
Ovarian oocytes of immature Bos gaurus were capable of in vitro maturation and fertilization with thawed homologous spermatozoa.
“Johnston et al. (1994) found that ovarian oocytes of immature Bos gaurus were capable of in vitro maturation and fertilization with thawed homologous spermatozoa and that the resulting embryos were capable of advancing to blastocysts in culture and of producing live-born offspring after embryo transfer.”
From Bos frontalis and Bos gaurus (Artiodactyla: Bovidae) (2018) · cites this paper as Johnston et al. 1994
A live-born calf was delivered 308 days after transferring the embryos to Holstein recipients.
“Specifically, the embryos developed to the blastocyst stage in 7 days, and a live-born calf was delivered 308 days after transferring the embryos to Holstein recipients.”
From Bos frontalis and Bos gaurus (Artiodactyla: Bovidae) (2018) · cites this paper as Johnston et al. 1994
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- Authors
- Johnston, L. A., Parrish, J. J., Monson, R., Leibfried-Rutledge, L., Susko-Parrish, J. L., Northey, D. L., Rutledge, J. J., & Simmons, L. G.
- Affiliations
- University of Wisconsin–Madison (US)
- ORCID
- Parrish, J. J.
- Venue
- Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 1994-01-01
- Volume
- 100
- Issue
- 1
- Pages
- 131-136
- ISSN
- 1470-1626 (ISSN-L)
- 1741-7899
- OpenAlex
- W2155454217
- Cited by
- 33
- References
- 3
- Citations by year
- 2025: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2014: 1 · 2013: 1 · 2012: 2
- FWCI
- 2.06
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Reproduction (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Reproductive Physiology in Livestock
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Keywords
- gaur, Bos gaurus, in vitro maturation, in vitro fertilization, embryonic development, oocyte nuclear maturation, endangered species conservation, embryo transfer, blastocyst
- MeSH terms
- Animals, Asia, Southeastern, Cattle, Cells, Cultured, Embryo Transfer, Female, Fertilization in Vitro, Embryonic and Fetal Development, Hybridization, Genetic, Oocytes, Oogenesis
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