The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes
Open accessAbstract
Homology-directed repair (HDR) of breaks induced by the RNA-programmed nuclease Cas9 has become a popular method for genome editing in several organisms. Most HDR protocols rely on plasmid-based expression of Cas9 and the gene-specific guide RNAs. Here we report that direct injection of in vitro–assembled Cas9-CRISPR RNA (crRNA) trans-activating crRNA (tracrRNA) ribonucleoprotein complexes into the gonad of Caenorhabditis elegans yields HDR edits at a high frequency. Building on our earlier finding that PCR fragments with 35-base homology are efficient repair templates, we developed an entirely cloning-free protocol for the generation of seamless HDR edits without selection. Combined with the co-CRISPR method, this protocol is sufficiently robust for use with low-efficiency guide RNAs and to generate complex edits, including ORF replacement and simultaneous tagging of two genes with fluorescent proteins.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
The new frontier of genome engineering with CRISPR-Cas9
Doudna, J. A., & Charpentier, E. (2014) · Science · doi:10.1126/science.1258096 · Cited by 7,062
CRISPR RNA Maturation by Trans-Encoded Small RNA and Host Factor RNase III
Deltcheva, E., Chylinski, K., Sharma, C. M., Gonzales, K., Chao, Y., Pirzada, Z. A., Eckert, M. R., Vogel, J., & Charpentier, E. (2011) · Nature · doi:10.1038/nature09886 · Cited by 2,563
Cited by
Applications of CRISPR technologies in research and beyond
Barrangou, R., & Doudna, J. A. (2016) · Nature Biotechnology · doi:10.1038/nbt.3659 · Cited by 1,005
Wang, H.-X., Li, M., Lee, C. M., Chakraborty, S., Kim, H.-W., Bao, G., & Leong, K. W. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00799 · Cited by 560
Genome-Editing Technologies: Principles and Applications
Gaj, T., Sirk, S. J., Shui, S.-L., & Liu, J. (2016) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a023754 · Cited by 381
Kitchen, S., Ratnappan, R., Han, S., Leasure, C., Grill, E., Iqbal, Z., Granger, O., O'Halloran, D. M., & Hawdon, J. M. (2019) · International Journal for Parasitology · doi:10.1016/j.ijpara.2018.12.004 · Cited by 105
Nuclear envelope assembly relies on CHMP-7 in the absence of BAF–LEM-mediated hole closure
Barger, S. R., Penfield, L., & Bahmanyar, S. (2023) · Journal of Cell Science · doi:10.1242/jcs.261385 · Cited by 17
Knudsen-Palmer, D. R., Raman, P., Ettefa, F., De Ravin, L., & Jose, A. M. (2024) · eLife · doi:10.7554/elife.97487 · Cited by 12
An NADH-controlled gatekeeper of ATP synthase
Schildhauer, F., Ryl, P. S. J., Lauer, S. M., Lenz, S., Barlas, A. B., Ouzounidis, V. R., Jeffrey, K., Marcu, D.-C., O’Reilly, F. J., Graziadei, A., et al. (2025) · Molecular Cell · doi:10.1016/j.molcel.2025.06.007 · Cited by 11
Structural insights into SSNA1 self-assembly and its microtubule binding for centriole maintenance
Agostini, L., Pfister, J. A., Basnet, N., Ding, J., Zhang, R., Biertümpfel, C., O’Connell, K. F., & Mizuno, N. (2025) · Nature Communications · doi:10.1038/s41467-025-62696-9 · Cited by 10
Shugarts Devanapally, N. M., Sathya, A., Yi, A. L., Chan, W. M., Marre, J. A., & Jose, A. M. (2025) · eLife · doi:10.7554/elife.99149.3 · Cited by 6
Hecquet, T., Arbogast, N., Suhner, D., Goetz, A., Amann, G., Yürekli, S., Marangoni, F., Quintin, S., Greve, J. N., Di Donato, N., et al. (2025) · iScience · doi:10.1016/j.isci.2025.113652 · Cited by 4
RG motifs promote piRNA-mediated gene silencing in C. elegans
Wallis, D. C., & Phillips, C. M. (2025) · Nucleic Acids Research · doi:10.1093/nar/gkaf1331 · Cited by 3
Knudsen-Palmer, D. R., Raman, P., Ettefa, F., De Ravin, L., & Jose, A. M. (2024) · eLife · doi:10.7554/elife.97487.3 · Cited by 2
A pilot study for whole proteome tagging in C. elegans
Eroglu, M., & Hobert, O. (2026) · doi:10.64898/2026.02.09.704846
Metadata
- Authors
- Paix, A., Folkmann, A., Rasoloson, D., & Seydoux, G.
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 2015-07-17
- Volume
- 201
- Issue
- 1
- Pages
- 47-54
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- OpenAlex
- W2120998017
- Cited by
- 675
- References
- 3
- FWCI
- 17.24
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
