Changes in the biogenic amine content of the prefrontal cortex, amygdala, dorsal hippocampus, and nucleus accumbens of rats submitted to single and repeated sessions of the elevated plus-maze test
Open accessAbstract
It has been demonstrated that exposure to a variety of stressful experiences enhances fearful reactions when behavior is tested in current animal models of anxiety. Until now, no study has examined the neurochemical changes during the test and retest sessions of rats submitted to the elevated plus maze (EPM). The present study uses a new approach (HPLC) by looking at the changes in dopamine and serotonin levels in the prefrontal cortex, amygdala, dorsal hippocampus, and nucleus accumbens in animals upon single or double exposure to the EPM (one-trial tolerance). The study involved two experiments: i) saline or midazolam (0.5 mg/kg) before the first trial, and ii) saline or midazolam before the second trial. For the biochemical analysis a control group injected with saline and not tested in the EPM was included. Stressful stimuli in the EPM were able to elicit one-trial tolerance to midazolam on re-exposure (61.01%). Significant decreases in serotonin contents occurred in the prefrontal cortex (38.74%), amygdala (78.96%), dorsal hippocampus (70.33%), and nucleus accumbens (73.58%) of the animals tested in the EPM (P < 0.05 in all cases in relation to controls not exposed to the EPM). A significant decrease in dopamine content was also observed in the amygdala (54.74%, P < 0.05). These changes were maintained across trials. There was no change in the turnover rates of these monoamines. We suggest that exposure to the EPM causes reduced monoaminergic neurotransmission activity in limbic structures, which appears to underlie the "one-trial tolerance" phenomenon.
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The release of monoamines such as serotonin at different nuclei in the corticolimbic network affects anxiety.
“the release of monoamines such as serotonin at different nuclei in the corticolimbic network affects anxiety”
From Resolving the neural circuits of anxiety (2015) · cites this paper as [141]
Innate anxiety-like behaviors in the elevated plus maze are associated with decreased levels of serotonin in the mPFC, amygdala and hippocampus.
“innate anxiety-like behaviors in the EPM are associated with decreased levels of serotonin in the mPFC, amygdala and hippocampus141”
From Resolving the neural circuits of anxiety (2015) · cites this paper as [141]
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- Authors
- Carvalho, M. C., Albrechet-Souza, L., Masson, S., & Brandão, M. L.
- Affiliations
- Universidade de São Paulo (BR)
- Universidade de São Paulo, Brasil
- Venue
- Brazilian Journal of Medical and Biological Research
- Publisher
- FapUNIFESP (SciELO)
- Published
- 2005-12
- Volume
- 38
- Issue
- 12
- Pages
- 1857-1866
- ISSN
- 0100-879X (ISSN-L)
- OpenAlex
- W2123868630
- Cited by
- 41
- References
- 37
- Citations by year
- 2026: 1 · 2021: 3 · 2020: 2 · 2019: 1 · 2017: 4 · 2015: 2 · 2014: 7 · 2013: 2 · 2012: 1
- FWCI
- 1.20
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Brazilian Journal of Medical and Biological Research (publishedVersion, cc-by)
- PubMed (publishedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
- Americanae (AECID Library) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo (02/03705-0)
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- elevated plus maze, serotonin, dopamine, biogenic amines, prefrontal cortex, amygdala, dorsal hippocampus, nucleus accumbens, anxiety, stress, monoaminergic neurotransmission, limbic system, rats
- MeSH terms
- Amygdala, Animals, Anxiety, Brain, Chromatography, High Pressure Liquid, Dopamine, Hippocampus, Male, Midazolam, Nucleus Accumbens, Serotonin, Anti-Anxiety Agents, Rats, Wistar, Prefrontal Cortex, Maze Learning, Rats
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