Cotton plants expressing a hemipteran-active Bacillus thuringiensis crystal protein impact the development and survival of Lygus hesperus (Hemiptera: Miridae) nymphs
Abstract
The plant bugs Lygus hesperus Knight (Hemiptera: Miridae) and L. lineolaris (Palisot de Beauvois) have emerged as economic pests of cotton in the United States. These hemipteran species are refractory to the insect control traits found in genetically modified commercial varieties of cotton. In this article, we report the isolation and characterization of a 35 kDa crystal protein from Bacillus thuringiensis, designated TIC807, which causes reduced mass gain and mortality of L. hesperus and L. lineolaris nymphs when presented in an artificial diet feeding assay. Cotton plants expressing the TIC807 protein were observed to impact the survival and development of L. hesperus nymphs in a concentration-dependent manner. These results, demonstrating in planta activity of a Lygus insecticidal protein, represent an important milestone in the development of cotton varieties protected from Lygus feeding damage.
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Modified Cry51Aa2 protein (Cry51Aa2.834_16) protects cotton against feeding damage caused by hemipteran and thysanopteran pests.
“An example is modified Cry51Aa2 protein (Cry51Aa2.834_16)that protects cotton against feeding damage caused by hemipteran and thysanopteran pests (Baum et al., 2012;Gowda et al., 2016;Bachman et al., 2017).”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Baum et al., 2012
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Genetically engineered crops help support conservation biological control
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Self-citation
- Authors
- Baum, J. A., Sukuru, U. R., Penn, S. R., Meyer, S. E., Subbarao, S., Shi, X., Flasinski, S., Heck, G. R., Brown, R. S., & Clark, T. L.
- Affiliations
- Monsanto (United States) (US)
- ORCID
- Clark, Thomas L.
- Venue
- Journal of Economic Entomology
- Publisher
- Oxford University Press (OUP)
- Published
- 2012-04-01
- Volume
- 105
- Issue
- 2
- Pages
- 616-624
- ISSN
- 0022-0493 (ISSN-L)
- OpenAlex
- W2146568189
- Cited by
- 86
- References
- 37
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 9 · 2023: 8 · 2022: 8 · 2021: 1 · 2020: 4 · 2019: 8 · 2018: 10 · 2017: 5 · 2016: 8 · 2015: 6 · 2014: 7 · 2013: 5
- FWCI
- 2.31
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Economic Entomology (publishedVersion)
- PubMed (publishedVersion)
- BioOne Complete (BioOne) (submittedVersion)
- Funders
- State of New Jersey Department of Agriculture
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Hemiptera Insect Studies
- Keywords
- Lygus hesperus, Lygus lineolaris, Bacillus thuringiensis, crystal proteins, cotton pests, insecticidal proteins, nymphal development, nymph survival, mass gain, transgenic cotton, plant-insect interactions, Miridae
- MeSH terms
- Bacillus thuringiensis Toxins, Animals, Bacillus thuringiensis, Bacterial Proteins, Gossypium, Diet, Endotoxins, Hemolysin Proteins, Nymph, Pest Control, Biological, Species Specificity, Heteroptera, Plants, Genetically Modified
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