Mangiferin Inhibits Inflammation and Cell Proliferation, and Activates Proapoptotic Events via NF-κB Inhibition in DMBA-Induced Mammary Carcinogenesis in Rats
Abstract
The purpose of this study was to investigate the anti-inflammatory, antiproliferatiive, and proapoptotic molecular mechanisms of mangiferin (MGN) against mammary carcinogenesis induced by 7,12-dimethylbenz(a)anthracene (DMBA). Mammary cancer in rats was induced by single-dose subcutaneous injection of 0.5 ml DMBA (80 mg/kg in sesame oil) in the mammary gland. Increased tumor incidence and volume and other tumorigenic properties were observed. Further, we observed in these rats reduced antioxidant enzyme activity and elevated thiobarbituric acid reactive substance (TBARS) levels in plasma and tissues. DMBA-induced rats shows enhanced expression of the inflammatory markers NF-κBp65, COX-2, and iNOS and proliferation of PCNA and Cyclin D1, and overexpression of the antiapoptotic marker Bcl-2. Mangiferin (100 mg/kg body weight), administered orally once per day, significantly enhanced (p < 0.05) antioxidant levels and reduced TBARS levels. Moreover, MGN inhibited NF-κBp65 nucleus transcriptional activation, thereby suppressing inflammation and cell proliferation, and it increased proapoptotic proteins. Apoptosis was confirmed by TUNEL assay. In summary, MGN suppressed DMBA-induced mammary carcinogenesis through enhanced antioxidant levels, NF-κB inhibition, and positive regulation of apoptotic signals.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In vivo studies in rats showed that MGF enhanced antioxidant enzyme levels and inhibited NF-κB p65 activation, reducing inflammation and cell proliferation.
“in vivo studies demonstrated that MGF, administrated in rats, enhanced both antioxidant enzyme levels and inhibited NF-κB p65 activation, showing a significant reduction of inflammation and cell proliferation [40,41].”
From Effects of Mangiferin on LPS-Induced Inflammation and SARS-CoV-2 Viral Adsorption in Human Lung Cells (2022) · cites this paper as [40]
Cites
Still being checked.
Cited by
Sorted by citations
Thioredoxin-interacting protein (TXNIP) as a target for Alzheimer’s disease: flavonoids and phenols
Zhang, M., Hu, G., Shao, N., Qin, Y.-P., Chen, Q., Wang, Y., Zhou, P., & Cai, B. (2021) · Inflammopharmacology · doi:10.1007/s10787-021-00861-4 · Cited by 46 · see citing works
Li, B., Wang, Y., Zhao, H., Yin, K., Liu, Y., Wang, D., Zong, H., & Xing, M. (2021) · Environmental Science and Pollution Research · doi:10.1007/s11356-021-17197-9 · Cited by 24 · see citing works
Role of Mangiferin in Management of Cancers through Modulation of Signal Transduction Pathways
Rahmani, A. H., Almatroudi, A., Allemailem, K. S., Alharbi, H. O. A., Alwanian, W. M., Alhunayhani, B. A., Algahtani, M., Theyab, A., Almansour, N. M., Algefary, A. N., et al. (2023) · Biomedicines · doi:10.3390/biomedicines11123205 · Cited by 22 · see citing works
Iqbal, H., Inam‐Ur‐Raheem, M., Munir, S., Rabail, R., Kafeel, S., Shahid, A., Mousavi Khaneghah, A., & Aadil, R. M. (2024) · Food Science & Nutrition · doi:10.1002/fsn3.3869 · Cited by 19 · see citing works
Gu, Y., Hu, Y., Huang, S., Ruiz, S., Kawai, T., Bai, Y., & Han, X. (2023) · Biology · doi:10.3390/biology12070976 · Cited by 15 · see citing works
Spampinato, M., Carota, G., Sferrazzo, G., Fuochi, V., Distefano, A., Ronsisvalle, S., Sipala, F., Giuffrida, R., Furneri, P. M., Di Rosa, M., et al. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14122845 · Cited by 14 · see citing works
“In this regard, in vivo studies demonstrated that MGF, administrated in rats, enhanced both antioxidant enzyme levels and inhibited NF-κB p65 activation, showing a significant reduction of inflammation and cell proliferation [40,41].”
BackgroundMangiferin: Sources, Anti-Inflammatory Activities, and Molecular Mechanisms
Shang, Y., Tian, J., Zhang, Z., Luo, L., Saeheng, S., Qiu, S., Zhou, R., Chen, J., & Li, L. (2025) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.5c06234 · Cited by 12 · see citing works
Effect of erucic acid against DMBA-induced breast cancer via inflammatory/apoptosis pathway
Alamri, A. M., Al-Abbasi, F. A., Afzal, M., Alqurashi, M. M., Eid, T. M., Albishi, H. M., Moglad, E. H. O., Sayyed, N., & Kazmi, I. A. (2025) · Food Bioscience · doi:10.1016/j.fbio.2025.107508 · Cited by 6 · see citing works
Zhang, W., Yue, B., Zhao, F., Zhi, Y., Wang, X., Yang, S., Sun, D., Lv, N., & Wu, X. (2023) · Toxicology Mechanisms and Methods · doi:10.1080/15376516.2023.2223291 · Cited by 6 · see citing works
Self-citationChakraborty, P., Das, A., Chatterjee, S., Bairagi, A., Bhattacharya, H., Bhattacharyya, C., Chatterjee, N., Sil, P. C., & Dewanjee, S. (2025) · Naunyn-Schmiedeberg s Archives of Pharmacology · doi:10.1007/s00210-025-04068-0 · Cited by 4 · see citing works
Zingue, S., Fongang, Y. S. F., Ossomba, E. R., Tsapi, V. B. T., Silihe, K. K., Mbou, W. D., Fogang, B., René, E., Chouna, J. R., Njamen, D., et al. (2024) · Heliyon · doi:10.1016/j.heliyon.2024.e35000 · Cited by 3 · see citing works
Royal jelly attenuates DMBA induced preneoplastic lesions in rat mammary gland and Skin
Salehi, A., Yaghoobi, F., Hosseini, S. M., & Hashemi, S. (2026) · Scientific Reports · doi:10.1038/s41598-026-44968-6 · Cited by 2 · see citing works
Tueche, A. B., Kamdem, M. H. K., Tonga, J. L., Mmutlane, E. M., Ndinteh, D. T., Zingue, S., & Njamen, D. (2026) · Frontiers in Pharmacology · doi:10.3389/fphar.2026.1772878
- Authors
- Wang, X., Yuwen, T., & Yanqin, T.
- ORCID
- Wang, Xin
- Venue
- Journal of Environmental Pathology, Toxicology and Oncology
- Publisher
- Begell House
- Published
- 2021
- Volume
- 40
- Issue
- 2
- Pages
- 1-9
- ISSN
- 0731-8898 (ISSN-L)
- OpenAlex
- W3120494125
- Cited by
- 13
- References
- 0
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 1 · 2023: 4 · 2022: 1 · 2021: 2
- FWCI
- 2.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Environmental Pathology Toxicology and Oncology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Mangiferin and Mango Extracts
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Natural Compound Pharmacology Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Natural product bioactivities and synthesis
- Keywords
- mangiferin, mammary carcinogenesis, DMBA-induced mammary tumors, NF-κB inhibition, apoptosis, inflammation, cell proliferation, antioxidant enzymes, lipid peroxidation, cyclooxygenase-2, inducible nitric oxide synthase, Bcl-2, cyclin D1, PCNA, chemoprevention
- MeSH terms
- Animals, Anti-Inflammatory Agents, Antineoplastic Agents, Carcinogens, Female, Mammary Glands, Animal, Mammary Neoplasms, Experimental, 9,10-Dimethyl-1,2-benzanthracene, NF-kappa B, Rats, Sprague-Dawley, Apoptosis, Xanthones, Cell Proliferation, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
