The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.
Lamin A/C Expression Is a Marker of Mouse and Human Embryonic Stem Cell Differentiation
Abstract
Nuclear lamins comprise the nuclear lamina, a scaffold-like structure that lines the inner nuclear membrane. B-type lamins are present in almost all cell types, but A-type lamins are expressed predominantly in differentiated cells, suggesting a role in maintenance of the differentiated state. Previous studies have shown that lamin A/C is not expressed during mouse development before day 9, nor in undifferentiated mouse embryonic carcinoma cells. To further investigate the role of lamins in cell phenotype maintenance and differentiation, we examined lamin expression in undifferentiated mouse and human embryonic stem (ES) cells. Wide-field and confocal immunofluorescence microscopy and semiquantitative reverse transcription–polymerase chain reaction analysis revealed that undifferentiated mouse and human ES cells express lamins B1 and B2 but not lamin A/C. Mouse ES cells display high levels of lamins B1 and B2 localized both at the nuclear periphery and throughout the nucleoplasm, but in human ES cells, B1 and B2 expression is dimmer and localized primarily at the nuclear periphery. Lamin A/C expression is activated during human ES cell differentiation before downregulation of the pluripotency marker Oct-3/4 but not before the downregulation of the pluripotency markers Tra-1-60, Tra-1-81, and SSEA-4. Our results identify the absence of A-type lamin expression as a novel marker for undifferentiated ES cells and further support a role for nuclear lamins in cell maintenance and differentiation.
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Cites
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Cited by
Dechat, T., Pfleghaar, K., Sengupta, K., Shimi, T., Shumaker, D. K., Solimando, L., & Goldman, R. D. (2008) · Genes & Development · doi:10.1101/gad.1652708 · Cited by 940
Physical plasticity of the nucleus in stem cell differentiation
Pajerowski, J. D., Dahl, K. N., Zhong, F. L., Sammak, P. J., & Discher, D. E. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0702576104 · Cited by 833
Self-citationHuman iPSC-based modeling of late-onset disease via progerin-induced aging
Miller, J. D., Ganat, Y. M., Kishinevsky, S., Bowman, R. L., Liu, B., Tu, E. Y., Mandal, P. K., Vera, E., Shim, J.-W., Kriks, S., et al. (2013) · Cell stem cell · doi:10.1016/j.stem.2013.11.006 · Cited by 777
Nuclear Shape, Mechanics, and Mechanotransduction
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Recapitulation of premature ageing with iPSCs from Hutchinson-Gilford progeria syndrome
Liu, G.-H., Barkho, B. Z., Ruiz, S., Diep, D., Qu, J., Yang, S.-L., Panopoulos, A. D., Suzuki, K., Kurian, L., Walsh, C., et al. (2011) · Nature · doi:10.1038/nature09879 · Cited by 575
Metadata
- Authors
- Constantinescu, D., Gray, H. L., Sammak, P. J., Schatten, G. P., & Csoka, A. B.
- Venue
- Stem Cells
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-01-01
- Volume
- 24
- Issue
- 1
- Pages
- 177-185
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W2165974961
- Cited by
- 355
- References
- 53
- FWCI
- 3.57
- Type
- journal article
- Language
- en
- Open access
- closed
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- Constantinescu, D., Gray, H. L., Sammak, P. J., Schatten, G. P., & Csoka, A. B. (2006). Lamin A/C Expression Is a Marker of Mouse and Human Embryonic Stem Cell Differentiation. Stem Cells. https://doi.org/10.1634/stemcells.2004-0159
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