The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Activin A Maintains Pluripotency of Human Embryonic Stem Cells in the Absence of Feeder Layers
Open accessAbstract
To date, all human embryonic stem cells (hESCs) available for research require unidentified soluble factors secreted from feeder layers to maintain the undifferentiated state and pluripotency. Activation of STAT3 by leukemia inhibitory factor is required to maintain "stemness" in mouse embryonic stem cells, but not in hESCs, suggesting the existence of alternate signaling pathways for self-renewal and pluripotency in human cells. Here we show that activin A is secreted by mouse embryonic feeder layers (mEFs) and that culture medium enriched with activin A is capable of maintaining hESCs in the undifferentiated state for >20 passages without the need for feeder layers, conditioned medium from mEFs, or STAT3 activation. hESCs retained both normal karyotype and markers of undifferentiated cells, including Oct-4, nanog, and TRA-1-60 and remained pluripotent, as shown by the in vivo formation of teratomas.
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Cites
Embryonic Stem Cell Lines Derived from Human Blastocysts
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Cited by
Efficient differentiation of human embryonic stem cells to definitive endoderm
D'Amour, K. A., Agulnick, A. D., Eliazer, S., Kelly, O. G., Kroon, E., & Baetge, E. E. (2005) · Nature Biotechnology · doi:10.1038/nbt1163 · Cited by 1,730
TGF-β and the TGF-β family: context-dependent roles in cell and tissue physiology
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Feeder-independent culture of human embryonic stem cells
Ludwig, T. E., Bergendahl, V., Levenstein, M. E., Yu, J., Probasco, M. D., & Thomson, J. A. (2006) · Nature Methods · doi:10.1038/nmeth902 · Cited by 681
Xiao, L., Yuan, X., & Sharkis, S. J. (2006) · Stem Cells · doi:10.1634/stemcells.2005-0299 · Cited by 353
Yu, J., & Thomson, J. A. (2008) · Genes & Development · doi:10.1101/gad.1689808 · Cited by 342
Metadata
- Authors
- Beattie, G. M., Lopez, A. D., Bucay, N., Hinton, A., Firpo, M. T., King, C. C., & Hayek, A.
- Venue
- STEM CELLS
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-04
- Volume
- 23
- Issue
- 4
- Pages
- 489-495
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W2160266371
- Cited by
- 503
- References
- 28
- FWCI
- 17.63
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Beattie, G. M., Lopez, A. D., Bucay, N., Hinton, A., Firpo, M. T., King, C. C., & Hayek, A. (2005). Activin A Maintains Pluripotency of Human Embryonic Stem Cells in the Absence of Feeder Layers. STEM CELLS. https://doi.org/10.1634/stemcells.2004-0279
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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