Heat Shock 70‐kDa Protein 8 Isoform 1 Is Expressed on the Surface of Human Embryonic Stem Cells and Downregulated upon Differentiation

Son, Yeon Sung; Park, Jae Hyun; Kang, Young Kook; Park, Jin‐Sung; Choi, Hong Seo; Lim, Ji Young; Lee, Jeoung Eun; Lee, Jung Bok; Ko, Myoung Seok; Kim, Yong‐Sam; Ko, Jeong‐Heon; Yoon, Hyun Soo; Lee, Kwang‐Woong; Seong, Rho Hyun; Moon, Shin Yong; Ryu, Chun Jeih; Hong, Hyo Jeong

doi:10.1634/stemcells.2004-0307

Cited by 54Pluripotent Stem Cells ResearchDevelopmental Biology and Gene RegulationMesenchymal stem cell research

Heat Shock 70‐kDa Protein 8 Isoform 1 Is Expressed on the Surface of Human Embryonic Stem Cells and Downregulated upon Differentiation

Son, Yeon Sung, Park, Jae Hyun, Kang, Young Kook, Park, Jin‐Sung, Choi, Hong Seo, Lim, Ji Young, Lee, Jeoung Eun, Lee, Jung Bok +9 more·2005·STEM CELLS

DOI 10.1634/stemcells.2004-0307

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Abstract

The cell-surface markers used routinely to define the undifferentiated state and pluripotency of human embryonic stem cells (hESCs) are those used in mouse embryonic stem cells (mESCs) because of a lack of markers directly originated from hESC itself. To identify more hESC-specific cell-surface markers, we generated a panel of monoclonal antibodies (MAbs) by immunizing the irradiated cell clumps of hESC line Miz-hES1, and selected 26 MAbs that were able to bind to Miz-hES1 cells but not to mESCs, mouse embryonic fibroblast cells, and STO cells. Most antibodies did not bind to human neural progenitor cells derived from the Miz-hES1 cells, either. Of these, MAb 20-202S (IgG1, kappa) immunoprecipitated a cell-surface protein of 72-kDa from the lysate of biotin-labeled Miz-hES1 cells, which was identified to be heat shock 70-kDa protein 8 isoform 1 (HSPA8) by quadrupole time-of-flight tandem mass spectrometry. Immunocytochemical analyses proved that the HSPA8 protein was also present on the surface of hESC lines Miz-hES4, Miz-hES6, and HSF6. Two-color flow cytometric analysis of Miz-hES1 and HSF6 showed the coexpression of the HSPA8 protein with other hESC markers such as stage-specific embryonic antigen 3 (SSEA3), SSEA4, TRA-1-60, and TRA-1-81. Flow cytometric and Western blot analyses using various cells showed that MAb 20-202S specifically bound to the HSPA8 protein on the surface of Miz-hES1, contrary to other anti-HSP70 antibodies examined. Furthermore, the surface expression of the HSPA8 protein on Miz-hES1 was markedly downregulated upon differentiation. These data indicate that a novel MAb 20-202S recognizes the HSPA8 protein on the surface of hESCs and suggest that the HSPA8 protein is a putative cell-surface marker for undifferentiated hESCs.

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HSPA8 was significantly co-expressed with integrin alpha V, annexin I and other ECM components.
“Also, the heat shock protein 8 (HSPA8), a surface marker for the undifferentiated cellular state expressed on the surface of human embryonic stem cells [39], performs an important role in the repair processes following harmful tissular assaults (for example, hemorrhage or local ischemia) [40], and was found to be significantly co-expressed with integrin alpha V, annexin I and other ECM components.”
From Adipose tissue transcriptomic signature highlights the pathological relevance of extracellular matrix in human obesity (2008) · cites this paper as [39]

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Authors: Son, Y. S., Park, J. H., Kang, Y. K., Park, J., Choi, H. S., Lim, J. Y., Lee, J. E., Lee, J. B., Ko, M. S., Kim, Y., Ko, J., Yoon, H. S., Lee, K., Seong, R. H., Moon, S. Y., Ryu, C. J., & Hong, H. J.
Affiliations: Seoul National University (KR)
Laboratory of Antibody Engineering, Korea Research Institute of Bioscience and Biotechnology, Daejon, Korea
Korea Research Institute of Bioscience and Biotechnology (KR)
MizMedi Hospital (KR)
Republic of Korea Army (KR)
Hanyang University (KR)
Corresponding author: Ryu, Chun Jeih
Hong, Hyo Jeong
ORCID: Son, Yeon Sung
Park, Jin‐Sung
Lee, Jeoung Eun
Lee, Jung Bok
Ko, Myoung Seok
Kim, Yong‐Sam
Ko, Jeong‐Heon
Lee, Kwang‐Woong
Seong, Rho Hyun
Ryu, Chun Jeih
Venue: STEM CELLS
Publisher: Oxford University Press (OUP)
Published: 2005-11
Volume: 23
Issue: 10
Pages: 1502-1513
ISSN: 1066-5099 (ISSN-L)
1549-4918
DOI: 10.1634/stemcells.2004-0307
OpenAlex: W1979112028
Cited by: 54
References: 53
Citations by year: 2026: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 2 · 2019: 3 · 2017: 1 · 2016: 2 · 2015: 3 · 2014: 3 · 2012: 3
FWCI: 2.13
Type: journal article
Language: en
Open access: closed
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
doi.wiley.com/10.1002/tdm_license_1.1 (tdm)
Locations: Stem Cells (publishedVersion)
PubMed (publishedVersion)
KRIBB Repository (submittedVersion)
Seoul National University Open Repository (Seoul National University) (submittedVersion)
Funders: Korea Basic Science Institute
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
Keywords: human embryonic stem cells, Hsc70, cell surface markers, pluripotency, monoclonal antibodies, differentiation, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81
MeSH terms: Antibodies, Monoclonal, Antigens, Surface, Cell Differentiation, Cell Line, HeLa Cells, Humans, Immunohistochemistry, Mass Spectrometry, Stem Cells, Down-Regulation, HSP70 Heat-Shock Proteins, Protein Isoforms, Embryo Research

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Heat Shock 70‐kDa Protein 8 Isoform 1 Is Expressed on the Surface of Human Embryonic Stem Cells and Downregulated upon Differentiation

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