The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Directed Differentiation of Ventral Spinal Progenitors and Motor Neurons from Human Embryonic Stem Cells by Small Molecules
Open accessAbstract
Specification of distinct cell types from human embryonic stem cells (hESCs) is key to the potential application of these naïve pluripotent cells in regenerative medicine. Determination of the nontarget differentiated populations, which is lacking in the field, is also crucial. Here, we show an efficient differentiation of motor neurons (∼50%) by a simple sequential application of retinoid acid and sonic hedgehog (SHH) in a chemically defined suspension culture. We also discovered that purmorphamine, a small molecule that activates the SHH pathway, could replace SHH for the generation of motor neurons. Immunocytochemical characterization indicated that cells differentiated from hESCs were nearly completely restricted to the ventral spinal progenitor fate (NKX2.2+, Irx3+, and Pax7−), with the exception of motor neurons (HB9+) and their progenitors (Olig2+). Thus, the directed neural differentiation system with small molecules, even without further purification, will facilitate basic and translational studies using human motoneurons at a minimal cost. Disclosure of potential conflicts of interest is found at the end of this article.
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Self-citationDirected Differentiation of Embryonic Stem Cells into Motor Neurons
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Neural progenitors from human embryonic stem cells
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Stem cells in human neurodegenerative disorders — time for clinical translation?
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Modeling ALS with iPSCs reveals that mutant SOD1 misregulates neurofilament balance in motor neurons
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Self-citationDifferentiation of spinal motor neurons from pluripotent human stem cells
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Metadata
- Authors
- Li, X.-J., Hu, B.-Y., Jones, S. A., Zhang, Y.-S., LaVaute, T., Du, Z.-W., & Zhang, S.-C.
- Venue
- Stem Cells
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-01-31
- Volume
- 26
- Issue
- 4
- Pages
- 886-893
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W2011850308
- Cited by
- 278
- References
- 37
- FWCI
- 10.17
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Li, X.-J., Hu, B.-Y., Jones, S. A., Zhang, Y.-S., LaVaute, T., Du, Z.-W., & Zhang, S.-C. (2008). Directed Differentiation of Ventral Spinal Progenitors and Motor Neurons from Human Embryonic Stem Cells by Small Molecules. Stem Cells. https://doi.org/10.1634/stemcells.2007-0620
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