The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
The Transcriptome Profile of Human Embryonic Stem Cells as Defined by SAGE
Abstract
Human embryonic stem (ES) cell lines that have the ability to self-renew and differentiate into specific cell types have been established. The molecular mechanisms for self-renewal and differentiation, however, are poorly understood. We determined the transcriptome profiles for two proprietary human ES cell lines (HES3 and HES4, ES Cell International), and compared them with murine ES cells and other human tissues. Human and mouse ES cells appear to share a number of expressed gene products although there are numerous notable differences, including an inactive leukemia inhibitory factor pathway and the high preponderance of several important genes like POU5F1 and SOX2 in human ES cells. We have established a list of genes comprised of known ES-specific genes and new candidates that can serve as markers for human ES cells and may also contribute to the "stemness" phenotype. Of particular interest was the downregulation of DNMT3B and LIN28 mRNAs during ES cell differentiation. The overlapping similarities and differences in gene expression profiles of human and mouse ES cells provide a foundation for a detailed and concerted dissection of the molecular and cellular mechanisms governing their pluripotency, directed differentiation into specific cell types, and extended ability for self-renewal.
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Cites
Embryonic Stem Cell Lines Derived from Human Blastocysts
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Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro
Spence, J. R., Mayhew, C. N., Rankin, S. A., Kuhar, M. F., Vallance, J. E., Tolle, K., Hoskins, E. E., Kalinichenko, V. V., Wells, S. I., Zorn, A. M., et al. (2011) · Nature · doi:10.1038/nature09691 · Cited by 1,922
TUT4 in Concert with Lin28 Suppresses MicroRNA Biogenesis through Pre-MicroRNA Uridylation
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Mono-Uridylation of Pre-MicroRNA as a Key Step in the Biogenesis of Group II let-7 MicroRNAs
Heo, I., Ha, M., Lim, J., Yoon, M.-J., Park, J.-E., Kwon, S. C., Chang, H., & Kim, V. N. (2012) · Cell · doi:10.1016/j.cell.2012.09.022 · Cited by 305
Good Cell Culture Practice for stem cells and stem-cell-derived models
Pamies, D. (2016) · ALTEX · doi:10.14573/altex.1607121 · Cited by 160
Lee, Y. T., de Vasconcellos, J. F., Yuan, J., Byrnes, C., Noh, S.-J., Meier, E. R., Kim, K. S., Rabel, A., Kaushal, M., Muljo, S. A., et al. (2013) · Blood · doi:10.1182/blood-2012-12-472308 · Cited by 142
Metadata
- Authors
- Richards, M., Tan, S., Tan, J., Chan, W., & Bongso, A.
- Venue
- STEM CELLS
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-01
- Volume
- 22
- Issue
- 1
- Pages
- 51-64
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W2149523673
- Cited by
- 435
- References
- 45
- FWCI
- 14.54
- Type
- journal article
- Language
- en
- Open access
- closed
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- Richards, M., Tan, S., Tan, J., Chan, W., & Bongso, A. (2004). The Transcriptome Profile of Human Embryonic Stem Cells as Defined by SAGE. STEM CELLS. https://doi.org/10.1634/stemcells.22-1-51
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