THE DEVELOPMENT OF A RADIOIMMUNOASSAY FOR OXYTOCIN: SENSITIVITY OF THE ASSAY IN AQUEOUS BUFFER SOLUTION, SPECIFICITY AND THE DISSOCIATION OF IMMUNOLOGICAL AND BIOLOGICAL ACTIVITY
Abstract
SUMMARY: A radioimmunoassay for oxytocin in aqueous solution is described, with a sensitivity comparable with the best current bioassays. It is highly specific; arginine-vasopressin and lysine-vasopressin interfere only at 1000-fold greater concentration, while bradykinin, histamine, acetycholine and many other substances, which interfere with some bioassays, have no effect. In certain circumstances, there is a dissociation between loss of biological and immunological activity. Thus reducing agents had no effect on immunological activity, in contrast to their effect on biological activity. In late pregnancy plasma, the biological activity of oxytocin is destroyed more rapidly than the immunological activity. Radioimmunoassays have considerable advantages over bioassays both in convenience and specificity. However, bioassays should be employed for reference purposes because of the dissociation between biological and immunological activity that may occur.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
By the 1980s, many laboratories had generated radioimmunoassays for oxytocin using independently produced antibodies, and most but not all required extraction.
“By the 1980s, many laboratories had generated radioimmunoassays for oxytocin, using independently produced antibodies 58, 72, 73, 74, 94, 95, 96, 97, 98] and most, but not all 99, 100, required extraction.”
From Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers (2016) · cites this paper as [73]
The human posterior pituitary contains approximately 21 μg of oxytocin, whereas that of the rat contains 0.5-1 μg.
“the human posterior pituitary contains approximately 21 μg of oxytocin 72, 73, 74, whereas that of the rat contains 0.5–1 μg.”
From Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers (2016) · cites this paper as [73]
Cited by
Sorted by citations
Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers
Leng, G., & Sabatier, N. (2016) · Journal of Neuroendocrinology · doi:10.1111/jne.12413 · Cited by 237 · see citing works
Release of Oxytocin and Vasopressin by the Human Foetus during Labour
Chard, T., Hudson, C. N., Edwards, C. R. W., & Boyd, N. R. H. (1971) · Nature · doi:10.1038/234352a0 · Cited by 224 · see citing works
Self-citationBiochemical Tests for the Assessment of Feto-Placental Function
Wilde, C. E., & OAKEY, R. E. (1975) · Annals of Clinical Biochemistry International Journal of Laboratory Medicine · doi:10.1177/000456327501200124 · Cited by 53 · see citing works
Gibbens, D., Boyd, N. R. H., Crocker, S. G., Baumber, S., & Chard, T. (1972) · BJOG An International Journal of Obstetrics & Gynaecology · doi:10.1111/j.1471-0528.1972.tb14215.x · Cited by 45 · see citing works
Self-citationSimultaneous radioimmunoassay of secretin and gastrin
Tai, H., & Chey, W. Y. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90305-5 · Cited by 44 · see citing works
OXYTOCIN RELEASE AND RENAL ACTIONS IN NORMAL AND BRATTLEBORO RATS*
BALMENT, R. J., Brimble, M. J., & Forsling, M. L. (1982) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1982.tb37432.x · Cited by 34 · see citing works
Self-citationSeppälä, M. T., Aho, I., Tissari, A. H., & Ruoslahti, E. I. (1972) · American Journal of Obstetrics and Gynecology · doi:10.1016/0002-9378(72)90902-7 · Cited by 34 · see citing works
Bisset, G. W. (1976) · doi:10.1007/978-1-349-02718-7_9 · Cited by 27 · see citing works
Radioimmunoassay of posterior pituitary peptides: A review
ROBINSON, A. G., & FRANTZ, A. G. (1973) · Metabolism · doi:10.1016/0026-0495(73)90224-2 · Cited by 24 · see citing works
Neurotensin and Related Substances
Carraway, R. E. (1978) · Elsevier eBooks · doi:10.1016/b978-0-12-379260-0.50015-7 · Cited by 21 · see citing works
RELEASE AND CLEARANCE OF NEUROPHYSINS AND POSTERIOR PITUITARY HORMONES*
Johnston, C. I., Hutchinson, J. S., Morris, B. J., & Dax, E. M. (1975) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1975.tb34191.x · Cited by 20 · see citing works
Hormone content of the neurohypophysis in foetal, new-born and adult guinea-pigs.
Am, B., & Forsling, M. L. (1972) · PubMed Central · Cited by 18 · see citing works
Self-citationRadioimmunoassay of Polypeptide Hormones and Enzymes
Felber, J. P. (1974) · Methods of biochemical analysis · doi:10.1002/9780470110423.ch1 · Cited by 17 · see citing works
Evidence for the presence of oxytocin in the corpus luteum of the goat
Homeida, A. M. (1986) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.1986.tb14584.x · Cited by 14 · see citing works
BIOASSAY OF OXYTOCIN ON THE SUPERFUSED MAMMARY GLAND OF THE MOUSE, USING AN AUTOMATIC APPARATUS
ROBINSON, I. C. A. F., & WALKER, J. M. (1974) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.1974.tb09617.x · Cited by 11 · see citing works
Oxytocin Release During Vaginal Distention in the Goat
Blank, M. S., & Debias, D. A. (1977) · Biology of Reproduction · doi:10.1095/biolreprod17.2.213 · Cited by 10 · see citing works
Ichihara, K., Yamamoto, T., AZUKIZAWA, M., & Miyai, K. (1979) · Clinica Chimica Acta · doi:10.1016/0009-8981(79)90169-4 · Cited by 8 · see citing works
Specificity of antibodies to arginine-vasopressin raised with succinylated poly-l-lysine as carrier
Vallotton, M. B. (1971) · Cellular and Molecular Life Sciences · doi:10.1007/bf02138176 · Cited by 5 · see citing works
The effect of pH on the selective permeability of the guinea-pig amnion to monovalent ions.
Foreman, P., & Mb, S. (1972) · PubMed Central · Cited by 3 · see citing works
A Luminescent Label for the Immunoassay of Oxytocin
Lequin, R. M., Moss, R. J., & Nispen, J. W. V. (1986) · Journal of Immunoassay · doi:10.1080/01971528608060464 · Cited by 2 · see citing works
Physikalisch-chemische Aspekte immunologischer und anderer reversibler Assoziations-Reaktionen
Kuss, E. (1984) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm.1984.22.12.851 · Cited by 2 · see citing works
Kagan, A., & Glick, S. M. (1978) · Elsevier eBooks · doi:10.1016/b978-0-12-379260-0.50022-4 · Cited by 2 · see citing works
- Authors
- CHARD, T., FORSLING, M. L., JAMES, M. A. R., KITAU, M. J., & LANDON, J.
- Venue
- Journal of Endocrinology
- Publisher
- Bioscientifica
- Published
- 1970-04-01
- Volume
- 46
- Issue
- 4
- Pages
- 533-542
- ISSN
- 0022-0795 (ISSN-L)
- 1479-6805
- OpenAlex
- W2034287189
- Cited by
- 22
- References
- 0
- Citations by year
- 2016: 1
- FWCI
- 8.83
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Pregnancy-related medical research
- Keywords
- oxytocin, radioimmunoassay, assay sensitivity, assay specificity, vasopressin, lysine vasopressin, bradykinin, histamine, acetylcholine, reducing agents, bioassay comparison
- MeSH terms
- Acetylcholine, Animals, Biological Assay, Bradykinin, Buffers, Female, Histamine, Iodine Isotopes, Mammary Glands, Animal, Methods, Oxidation-Reduction, Oxytocin, Pregnancy, Radioimmunoassay, Vasopressins, Water, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
