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Cited by 49Glaucoma and retinal disordersMedical and Biological SciencesVisual perception and processing mechanisms

Electron microscopy of the mouse optic nerve: a quantitative study of the total optic nerve fibers

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HONJIN, Ryohei, SAKATO, Shun-ichi, YAMASHITA, Toshio·1977·Archivum histologicum japonicum
DOI 10.1679/aohc1950.40.321
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Abstract

The total number of myelinated and unmyelinated nerve fibers was counted in a montage of electron micrographs prepared to cover the entire profile of the mouse optic nerve in transverse section. Many astrocytic processes form a barrier structure all around the optic nerve. As a whole, the mouse optic nerve consists of one fascicle where the nerve fibers, the glia cells and their processes, and the capillaries are fitted closely together to fill substantially all of the available space. No tissue space accompanied with connective tissue septa penetrates into the fascicle, except for those situated around the arteriole and venule. The total number of 64,746 myelinated nerve fibers in the optic nerve in one side was counted. This corresponds to about 98.8% of the total number of the optic nerve fibers. The total number of 807 unmyelinated nerve fibers was also counted. This corresponds to about 1.2% of the total nerve fibers. The external diameter of every myelinated fiber, including the myelin sheath, was measured on the montage micrograph and its frequency distribution was examined. The diameter of the myelinated fibers ranges from 0.3 to 4.2 mu and its mean value is 0.96 mu. The frequency distribution of fiber diameter is unimodal with a peak at 0.7 to 0.9 mu. A regional heterogeneity in fiber size is found in the optic nerve. The nerve fibers in the peripheral area of the nerve are relatively small and uniform in diameter, whereas those in the central area show a fairly wide range in diameter.

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  • In the 4-month-old mouse optic nerve, practically all axons are already myelinated.

    “We quantified OP division and OL production in different regions of the adult mouse CNS including the 4-month-old optic nerve, in which practically all axons are already myelinated.”

    From Oligodendrocyte Dynamics in the Healthy Adult CNS: Evidence for Myelin Remodeling (2013) · cites this paper as Honjin et al., 1977

    The Summary reports that 98.8% of optic nerve fibers were myelinated, supporting “practically all.” But Material and Methods identifies the studied mice as 6–8 months old, not 4 months old. The Discussion mentions a separate count of myelinated fibers at 4 months without establishing what proportion of axons were myelinated then. (LLM, conf=0.90)

    Experimental — accuracy not yet measured

  • There is a large disparity between the proportion of new myelin laid down in the optic nerve after P120 (approximately 6.5% of the total) and the proportion of axons that remains unmyelinated at that time (approximately 1%).

    “As shown in the following section, the total length of myelin sheath that is synthesized by adult-born OLs is similar to that synthesized by early-born OLs, so there is a large disparity between the proportion of new myelin that is laid down in the optic nerve after P120 (∼6.5% of the total) and the proportion of axons that remains unmyelinated at that time (∼1%) (Honjin et al., 1977; Dangata et a”

    From Oligodendrocyte Dynamics in the Healthy Adult CNS: Evidence for Myelin Remodeling (2013) · cites this paper as Honjin et al., 1977

  • 98.8% of optic nerve axons are myelinated in 6- to 8-month-old mice.

    “It has been estimated that 98.8% of optic nerve axons are myelinated in 6- to 8-month-old mice (Honjin et al., 1977).”

    From Oligodendrocyte Dynamics in the Healthy Adult CNS: Evidence for Myelin Remodeling (2013) · cites this paper as Honjin et al., 1977

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Research works printed but not linked: 1

  1. Yamadori, T.: The rotation and the pattern of localized distribution of the optic nerve fibers in rats. Proc. 10th Int. Congr. Anat., Kyoto Symp.: 39 (1975).

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Related

Authors
HONJIN, R., SAKATO, S.-I., & YAMASHITA, T.
Affiliations
  • Kanazawa University (JP)
  • Department of Anatomy and Neuro-Information Research Institute, Kanazawa University School of Medicine
Venue
Archivum histologicum japonicum
Publisher
International Society of Histology & Cytology
Published
1977
Volume
40
Issue
4
Pages
321-332
ISSN
0004-0681 (ISSN-L)
DOI
10.1679/aohc1950.40.321
OpenAlex
W2322930862
Cited by
49
References
19
Citations by year
2026: 1 · 2025: 4 · 2024: 3 · 2023: 3 · 2021: 1 · 2020: 3 · 2019: 1 · 2018: 4 · 2017: 2 · 2016: 3 · 2015: 3 · 2013: 2 · 2012: 1
FWCI
0.78
Type
journal article
Language
en
Open access
Locations
  • Archivum histologicum japonicum (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Ophthalmology › Glaucoma and retinal disorders
  • Health Sciences › Medicine › Anatomy › Medical and Biological Sciences
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Visual perception and processing mechanisms
Keywords
optic nerve, myelinated axons, unmyelinated nerve fibers, fiber diameter, myelin sheath, astrocytes, nerve fascicles, regional heterogeneity
MeSH terms
Animals, Axons, Male, Nerve Fibers, Nerve Fibers, Myelinated, Optic Nerve, Mice

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