Oligodendrocytes in the Mouse Corpus Callosum Maintain Axonal Function by Delivery of Glucose
Open accessAbstract
In the optic nerve, oligodendrocytes maintain axonal function by supplying lactate as an energy substrate. Here, we report that, in acute brain slices of the mouse corpus callosum, exogenous glucose deprivation (EGD) abolished compound action potentials (CAPs), which neither lactate nor pyruvate could prevent. Loading an oligodendrocyte with 20 mM glucose using a patch pipette prevented EGD-mediated CAP reduction in about 70% of experiments. Loading oligodendrocytes with lactate rescued CAPs less efficiently than glucose. In mice lacking connexin 47, oligodendrocyte filling with glucose did not prevent CAP loss, emphasizing the importance of glial networks for axonal energy supply. Compared with the optic nerve, the astrocyte network in the corpus callosum was less dense, and loading astrocytes with glucose did not prevent CAP loss during EGD. We suggest that callosal oligodendrocyte networks provide energy to sustain axonal function predominantly by glucose delivery, and mechanisms of metabolic support vary across different white matter regions.
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Pharmacologic manipulation of MCTs and glucose transporters gave uncertain answers about how axonal support was provided.
“however, pharmacologic manipulation of MCTs and glucose transporters gave uncertain answers about how axonal support was provided (Meyer et al., 2018).”
From Fueling thought: Management of glycolysis and oxidative phosphorylation in neuronal metabolism (2018) · cites this paper as Meyer et al., 2018
In corpus callosum white matter, exogenous lactate could not support axon function in the absence of glucose.
“however, in corpus callosum white matter, exogenous lactate could not support axon function in the absence of glucose (Meyer et al., 2018).”
From Fueling thought: Management of glycolysis and oxidative phosphorylation in neuronal metabolism (2018) · cites this paper as Meyer et al., 2018
In aglycemic conditions, glucose supplied to the gap junction-coupled network of oligodendrocytes was more effective than lactate in restoring axon function.
“In aglycemic conditions, glucose supplied to the gap junction–coupled network of oligodendrocytes was more effective than lactate in restoring axon function;”
From Fueling thought: Management of glycolysis and oxidative phosphorylation in neuronal metabolism (2018) · cites this paper as Meyer et al., 2018
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Fueling thought: Management of glycolysis and oxidative phosphorylation in neuronal metabolism
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“201803152 6 in corpus callosum white matter, exogenous lactate could not support axon function in the absence of glucose (Meyer et al., 2018).”
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- Authors
- Meyer, N., Richter, N., Fan, Z., Siemonsmeier, G., Pivneva, T., Jordan, P., Steinhäuser, C., Semtner, M., Nolte, C., & Kettenmann, H.
- Affiliations
- Max Delbrück Center (DE)
- Bogomoletz Institute of Physiology (UA)
- University of Bonn (DE)
- Corresponding author
- Kettenmann, Helmut
- ORCID
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2018-02
- Volume
- 22
- Issue
- 9
- Pages
- 2383-2394
- ISSN
- 2211-1247 (ISSN-L)
- OpenAlex
- W2788295856
- Cited by
- 145
- References
- 39
- Citations by year
- 2026: 8 · 2025: 14 · 2024: 21 · 2023: 14 · 2022: 21 · 2021: 22 · 2020: 20 · 2019: 13 · 2018: 12
- FWCI
- 7.62
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cell Reports (publishedVersion, cc-by)
- PubMed (publishedVersion)
- MDC Repository (Max-Delbrueck-Center for Molecular Medicine) (acceptedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Refubium (Universitätsbibliothek der Freien Universität Berlin) (submittedVersion)
- Universitätsbibliothek der FU Berlin Hochschulschriftenstelle u. Dokumentenserver
- Funders
- Deutsche Forschungsgemeinschaft (KE 329/28, STE 552/4)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Connexins and lens biology
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- oligodendrocytes, corpus callosum, glucose delivery, glucose deprivation, compound action potential, lactate, pyruvate, connexin 47, astrocytes, white matter, optic nerve, glial networks, axonal function
- MeSH terms
- Action Potentials, Animals, Astrocytes, Axons, Corpus Callosum, Dialysis, Glucose, Lysine, Neuroglia, Oligodendroglia, Connexins, Mice, Knockout, Pyruvic Acid, Lactic Acid, Monocarboxylic Acid Transporters
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