The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Glomerular Endothelial Glycocalyx Constitutes a Barrier to Protein Permeability
Abstract
Glycocalyx, composed of glycoproteins including proteoglycans, coats the luminal surface of the glomerular capillaries. Human heparanase degrades heparan sulphate glycosaminoglycans and is up-regulated in proteinuric states. In this study, we analyze the structure of the human glomerular endothelial cell glycocalyx in vitro and examine its functional relevance, especially after treatment with human heparanase. Electron microscopy of conditionally immortalized glomerular endothelial cells revealed a 200-nm thick glycocalyx over the plasma membrane, which was also demonstrated by confocal microscopy. Neuraminidase treatment removed the majority of glycocalyx, reduced trans-endothelial electrical resistance by 59%, and increased albumin flux by 207%. Heparinase III and human heparanase specifically cleaved heparan sulphate: this caused no change in trans-endothelial electrical resistance, but increased the albumin passage across the monolayers by 40% and 39%, respectively. Therefore, we have characterized the glomerular endothelial cell glycocalyx and have shown that it contributes to the barrier to flux of albumin across the cell layer. These results suggest an important role for this glycocalyx in the restriction of glomerular protein passage in vivo and suggest ways in which human heparanase levels may be linked to proteinuria in clinical disease.
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1 retracted
Wijnhoven, T. J. M., Lensen, J. F. M., Wismans, R. G., Lamrani, M., Monnens, L. A. H., Wevers, R. A., Rops, A. L. W. M. M., van der Vlag, J., Berden, J. H. M., van den Heuvel, L. P. W. J., et al. (2007) · Journal of the American Society of Nephrology · doi:10.1681/asn.2006070692 · Cited by 49
RetractedMaynard, S. E., Min, J.-Y., Merchan, J., Lim, K.-H., Li, J., Mondal, S., Libermann, T. A., Morgan, J. P., Sellke, F. W., Stillman, I. E., et al. (2003) · Journal of Clinical Investigation · doi:10.1172/jci17189 · Cited by 4,101
Eremina, V., Sood, M., Haigh, J., Nagy, A., Lajoie, G., Ferrara, N., Gerber, H.-P., Kikkawa, Y., Miner, J. H., & Quaggin, S. E. (2003) · Journal of Clinical Investigation · doi:10.1172/jci17423 · Cited by 1,313
A Conditionally Immortalized Human Podocyte Cell Line Demonstrating Nephrin and Podocin Expression
Saleem, M. A., O’Hare, M. J., Reiser, J., Coward, R. J., Inward, C. D., Farren, T., Xing, C. Y., Ni, L., Mathieson, P. W., & Mundel, P. (2002) · Journal of the American Society of Nephrology · doi:10.1681/asn.v133630 · Cited by 1,120
Self-citationPries, A. R., Secomb, T. W., & Gaehtgens, P. (2000) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/s004240000307 · Cited by 898
Mammalian heparanase: Gene cloning, expression and function in tumor progression and metastasis
Vlodavsky, I., Friedmann, Y., Elkin, M., Aingorn, H., Atzmon, R., Ishai-Michaeli, R., Bitan, M., Pappo, O., Peretz, T., Michal, I., et al. (1999) · Nature Medicine · doi:10.1038/10518 · Cited by 803
Vink, H., & Duling, B. R. (1996) · Circulation Research · doi:10.1161/01.res.79.3.581 · Cited by 615
Mechanotransduction and flow across the endothelial glycocalyx
Weinbaum, S., Zhang, X., Han, Y., Vink, H., & Cowin, S. C. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1332808100 · Cited by 579
Fine structures of capillary and endocapillary layer as revealed by ruthenium red.
Luft, J. H. (1966) · PubMed · Cited by 504
Endothelial Glycocalyx Damage Coincides With Microalbuminuria in Type 1 Diabetes
Nieuwdorp, M., Mooij, H. L., Kroon, J., Atasever, B., Spaan, J. A. E., Ince, C., Holleman, F., Diamant, M., Heine, R. J., Hoekstra, J. B. L., et al. (2006) · Diabetes · doi:10.2337/diabetes.55.04.06.db05-1619 · Cited by 442
Cited by
Proteinuria in diabetic kidney disease: A mechanistic viewpoint
Jefferson, J. A., Shankland, S. J., & Pichler, R. H. (2008) · Kidney International · doi:10.1038/ki.2008.128 · Cited by 419
Dengue Virus NS1 Disrupts the Endothelial Glycocalyx, Leading to Hyperpermeability
Puerta-Guardo, H., Glasner, D. R., & Harris, E. (2016) · PLOS Pathogens · doi:10.1371/journal.ppat.1005738 · Cited by 384
Satchell, S. C., & Braet, F. (2009) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.90601.2008 · Cited by 347
Self-citationHohenester, S., de Buy Wenniger, L. M., Paulusma, C. C., van Vliet, S. J., Jefferson, D. M., Oude Elferink, R. P., & Beuers, U. (2012) · Hepatology · doi:10.1002/hep.24691 · Cited by 315
Diabetes-Induced Reactive Oxygen Species: Mechanism of Their Generation and Role in Renal Injury
Fakhruddin, S., Alanazi, W., & Jackson, K. E. (2017) · Journal of Diabetes Research · doi:10.1155/2017/8379327 · Cited by 292
Metadata
- Authors
- Singh, A., Satchell, S. C., Neal, C. R., McKenzie, E. A., Tooke, J. E., & Mathieson, P. W.
- Venue
- Journal of the American Society of Nephrology
- Published
- 2007-11
- Volume
- 18
- Issue
- 11
- Pages
- 2885-2893
- ISSN
- 1046-6673 (ISSN-L)
- OpenAlex
- W2119802842
- Cited by
- 300
- References
- 39
- FWCI
- 7.06
- Type
- journal article
- Language
- en
- Open access
- closed
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- Singh, A., Satchell, S. C., Neal, C. R., McKenzie, E. A., Tooke, J. E., & Mathieson, P. W. (2007). Glomerular Endothelial Glycocalyx Constitutes a Barrier to Protein Permeability. Journal of the American Society of Nephrology. https://doi.org/10.1681/asn.2007010119
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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