The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
In vitro alpha-glucosidase and alpha-amylase enzyme inhibitory effects of Andrographis paniculata extract and andrographolide.
Open accessAbstract
There has been an enormous interest in the development of alternative medicines for type 2 diabetes, specifically screening for phytochemicals with the ability to delay or prevent glucose absorption. The goal of the present study was to provide in vitro evidence for potential inhibition of alpha-glucosidase and alpha-amylase enzymes, followed by a confirmatory in vivo study on rats to generate a stronger biochemical rationale for further studies on the ethanolic extract of Andrographis paniculata and andrographolide. The extract showed appreciable alpha-glucosidase inhibitory effect in a concentration-dependent manner (IC(50)=17.2+/-0.15 mg/ml) and a weak alpha-amylase inhibitory activity (IC(50)=50.9+/-0.17 mg/ml). Andrographolide demonstrated a similar (IC(50)=11.0+/-0.28 mg/ml) alpha-glucosidase and alpha-amylase inhibitory activity (IC(50)=11.3+/-0.29 mg/ml). The positive in vitro enzyme inhibition tests paved way for confirmatory in vivo studies. The in vivo studies demonstrated that A. paniculata extract significantly (P
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Cites
Ali, H., Houghton, P. J., & Soumyanath, A. (2006) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2006.04.004 · Cited by 773
Inhibition of Alpha-glucosidase and Amylase by Luteolin, a Flavonoid
KIM, J.-S., KWON, C.-S., & SON, K. H. (2000) · Bioscience, Biotechnology, and Biochemistry · doi:10.1271/bbb.64.2458 · Cited by 667
Apostolidis, E., Kwon, Y.-I., & Shetty, K. (2007) · Innovative Food Science & Emerging Technologies · doi:10.1016/j.ifset.2006.06.001 · Cited by 602
Anticancer and immunostimulatory compounds from Andrographis paniculata
Ajaya Kumar, R., Sridevi, K., Vijaya Kumar, N., Nanduri, S., & Rajagopal, S. (2004) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2004.03.004 · Cited by 527
Shen, Y., Chen, C., & Chiou, W.-F. (2002) · British Journal of Pharmacology · doi:10.1038/sj.bjp.0704493 · Cited by 358
Zhang, X., & Tan, B. K. (2000) · Clinical and Experimental Pharmacology and Physiology · doi:10.1046/j.1440-1681.2000.03253.x · Cited by 240
Antihyperglycemic Effect of Andrographolide in Streptozotocin-Induced Diabetic Rats
(2003) · Planta Medica · doi:10.1055/s-2003-45185 · Cited by 211
Amroyan, E., Gabrielian, E. S., Panossian, A. G., Wikman, G., & Wagner, H. (1999) · Phytomedicine · doi:10.1016/s0944-7113(99)80031-2 · Cited by 152
Antiangiogenic activity of Andrographis paniculata extract and andrographolide
Sheeja, K., Guruvayoorappan, C., & Kuttan, G. (2006) · International Immunopharmacology · doi:10.1016/j.intimp.2006.10.002 · Cited by 144
Singha, P. K., Roy, S., & Dey, S. (2006) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2006.10.026 · Cited by 139
Cited by
Kashtoh, H., & Baek, K.-H. (2022) · Plants · doi:10.3390/plants11202722 · Cited by 270
Phytotherapy in diabetes: Review on potential mechanistic perspectives
El-Abhar, H. S., & Schaalan, M. F. (2014) · World Journal of Diabetes · doi:10.4239/wjd.v5.i2.176 · Cited by 145
Natural Monoterpenes: Much More Than Only a Scent
Wojtunik‐Kulesza, K. A., Kasprzak, K., Oniszczuk, T., & Oniszczuk, A. (2019) · Chemistry & Biodiversity · doi:10.1002/cbdv.201900434 · Cited by 137
Mitra, A., De, B., Bhandari, K., Singla, R., Katakam, P., Samanta, T., Kushwaha, D., & Gundamaraju, R. (2015) · Pharmacognosy Magazine · doi:10.4103/0973-1296.172956 · Cited by 15
“Absorbance was calculated using the formula: α [(Ac+)–(Ac–)–(As–Ab)] amylase activity = × 100 [(Ac+)–(Ac–)] α-Glucosidase inhibitory assay The assay procedure was developed as described by Basak et al., 2010 and Subramanian et al., 2008, with slight modifications.”
Method
Metadata
- Authors
- Subramanian, R., Asmawi, M. Z., & Sadikun, A.
- Venue
- Acta Biochimica Polonica
- Publisher
- Frontiers Media SA
- Published
- 2008-05-29
- Volume
- 55
- Issue
- 2
- Pages
- 391-398
- ISSN
- 0001-527X (ISSN-L)
- 1734-154X
- OpenAlex
- W2159251461
- Cited by
- 363
- References
- 20
- FWCI
- 5.88
- Type
- journal article
- Language
- en
- Open access
- gold
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- Subramanian, R., Asmawi, M. Z., & Sadikun, A. (2008). In vitro alpha-glucosidase and alpha-amylase enzyme inhibitory effects of Andrographis paniculata extract and andrographolide. Acta Biochimica Polonica. https://doi.org/10.18388/abp.2008_3087
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