The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Long non-coding RNA PVT1 encapsulated in bone marrow mesenchymal stem cell-derived exosomes promotes osteosarcoma growth and metastasis by stabilizing ERG and sponging miR-183-5p
Open accessAbstract
Exosomes secreted by bone marrow mesenchymal stem cells (BMSCs) promote osteosarcoma cell proliferation and migration, while the underlying mechanism remains unknown. Since the long non-coding RNA PVT1 has been reported to be upregulated in osteosarcoma cells and contributes to its growth and metastasis, we aim to investigate whether BMSC-derived exosomes promote osteosarcoma growth and metastasis via transporting PVT1 into osteosarcoma cells. The PVT1 expression in BMSC-derived exosomes was markedly higher than that in osteosarcoma cell-derived exosomes. The co-culturing of BMSC-derived exosomes and osteosarcoma cells (Saos-2, MG-63, and MNNG/HOS cell lines) significantly raised PVT1 expression of osteosarcoma cells. The direct binding between PVT1 and the oncogenic protein ERG was confirmed using RNA immunoprecipitation and RNA pull-down assays, and the transported PVT1 promotes osteosarcoma cell proliferation and migration via inhibiting degradation and ubiquitination of ERG. PVT1 also increased ERG expression through sponging miR-183-5p. In summary, our findings indicated that BMSC-derived exosomes encapsulate PVTl and transport it into osteosarcoma cells, and the transported PVT1 promotes tumor growth and metastasis by inhibiting ubiquitination and promoting expression of ERG in osteosarcoma cells. These data provide a novel insight into the mechanism of BMSC-derived exosomes in affecting osteosarcoma progression.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source says what the claim attributes to it.
In the citing paper
“In another study, co-cultures of BM-MSC-derived exosomes with Saos-2, MG-63, and MNNG/HOS OS cells lines led to the increased expression of PVT1, a long, noncoding RNA that contributes to OS growth and metastasis [121].”
From Mesenchymal Stem/Stromal Cells: Immunomodulatory and Bone Regeneration Potential after Tumor Excision in Osteosarcoma Patients (2023) · cites this paper as [121]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
3 retracted
Chen, J., Yu, Y. H., Li, H., Hu, Q., Chen, X., He, Y., Xue, C., Ren, F., Ren, Z., Li, J., et al. (2019) · Molecular Cancer · doi:10.1186/s12943-019-0947-9 · Cited by 389
Self-citationRetractedHuang, F., Chen, W., Peng, J., Li, Y., Zhuang, Y., Zhu, Z., Shao, C., Yang, W., Yao, H., & Zhang, S. (2018) · Molecular Cancer · doi:10.1186/s12943-018-0845-6 · Cited by 134
RetractedWang, D., & Hu, Y. (2018) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2018.12.006 · Cited by 82
RetractedCommunication by Extracellular Vesicles: Where We Are and Where We Need to Go
Tkach, M., & Théry, C. (2016) · Cell · doi:10.1016/j.cell.2016.01.043 · Cited by 3,444
Endogenous microRNA sponges: evidence and controversy
Thomson, D. W., & Dinger, M. E. (2016) · Nature Reviews Genetics · doi:10.1038/nrg.2016.20 · Cited by 2,185
The biology and function of exosomes in cancer
Kalluri, R. (2016) · Journal of Clinical Investigation · doi:10.1172/jci81135 · Cited by 1,880
Human mesenchymal stem cells exert potent antitumorigenic effects in a model of Kaposi's sarcoma
Khakoo, A. Y., Pati, S., Anderson, S. A., Reid, W., Elshal, M. F., Rovira, I. I., Nguyen, A. T., Malide, D., Combs, C. A., Hall, G., et al. (2006) · The Journal of Experimental Medicine · doi:10.1084/jem.20051921 · Cited by 747
Hypoxic BMSC-derived exosomal miRNAs promote metastasis of lung cancer cells via STAT3-induced EMT
Zhang, X., Sai, B., Wang, F., Wang, L., Wang, Y., Zheng, L., Li, G., Tang, J., & Xiang, J. (2019) · Molecular Cancer · doi:10.1186/s12943-019-0959-5 · Cited by 560
Long Noncoding RNA in Cancer: Wiring Signaling Circuitry
Lin, C., & Yang, L. (2018) · Trends in Cell Biology · doi:10.1016/j.tcb.2017.11.008 · Cited by 488
MVP-mediated exosomal sorting of miR-193a promotes colon cancer progression
Teng, Y., Ren, Y., Hu, X., Mu, J., Samykutty, A., Zhuang, X., Deng, Z., Kumar, A., Zhang, L., Merchant, M. L., et al. (2017) · Nature Communications · doi:10.1038/ncomms14448 · Cited by 408
Mesenchymal stem cell exosomes: a two-edged sword in cancer therapy
Vakhshiteh, F., Atyabi, F., & Ostad, S. N. (2019) · International Journal of Nanomedicine · doi:10.2147/ijn.s200036 · Cited by 287
The oncogene ERG: a key factor in prostate cancer
Adamo, P., & Ladomery, M. (2015) · Oncogene · doi:10.1038/onc.2015.109 · Cited by 286
Viral effects on the content and function of extracellular vesicles
Raab‐Traub, N., & Dittmer, D. P. (2017) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2017.60 · Cited by 270
Concise Review: Adult Multipotent Stromal Cells and Cancer: Risk or Benefit?
Lazennec, G., & Jørgensen, C. (2008) · Stem Cells · doi:10.1634/stemcells.2007-1006 · Cited by 261
Shimbo, K., Miyaki, S., Ishitobi, H., Kato, Y., Kubo, T., Shimose, S., & Ochi, M. (2014) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2014.02.007 · Cited by 241
Tumor-originated exosomal lncUEGC1 as a circulating biomarker for early-stage gastric cancer
Lin, L., Li, Y., Zeng, Q., Wang, L., Chen, M.-L., Zhao, Z., Ye, G.-D., Luo, Q., Lv, P.-Y., Guo, Q., et al. (2018) · Molecular Cancer · doi:10.1186/s12943-018-0834-9 · Cited by 233
Exosome target cell selection and the importance of exosomal tetraspanins: a hypothesis
Rana, S., & Zöller, M. (2011) · Biochemical Society Transactions · doi:10.1042/bst0390559 · Cited by 224
Liu, R., Li, X., Zhu, W., Wang, Y., Zhao, D., Wang, X., Gurley, E. C., Liang, G., Chen, W., Lai, G., et al. (2019) · Hepatology · doi:10.1002/hep.30662 · Cited by 224
Gan, W., Dai, X., Lunardi, A., Li, Z., Inuzuka, H., Liu, P., Varmeh, S., Zhang, J., Liang Cheng, C., Sun, Y., et al. (2015) · Molecular Cell · doi:10.1016/j.molcel.2015.07.026 · Cited by 220
Qi, J., Zhou, Y., Jiao, Z., Wang, X., Zhao, Y., Li, Y., Chen, H., Yang, L., Zhu, H., & Li, Y. (2017) · Cellular Physiology and Biochemistry · doi:10.1159/000479998 · Cited by 191
Blocking Tumor-Educated MSC Paracrine Activity Halts Osteosarcoma Progression
Baglio, S. R., Lagerweij, T., Pérez-Lanzón, M., Ho, X. D., Léveillé, N., Melo, S. A., Cleton-Jansen, A.-M., Jordanova, E. S., Roncuzzi, L., Greco, M., et al. (2017) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-16-2726 · Cited by 187
Rao, V. N., Papas, T. S., & Reddy, E. S. P. (1987) · Science · doi:10.1126/science.3299708 · Cited by 177
Song, J., Wu, X., Liu, F., Li, M., Sun, Y., Wang, Y., Wang, C., Zhu, K., Jia, X., Wang, B., et al. (2017) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2017.06.024 · Cited by 175
Mesenchymal stroma: Role in osteosarcoma progression
Cortini, M., Avnet, S., & Baldini, N. (2017) · Cancer Letters · doi:10.1016/j.canlet.2017.07.024 · Cited by 171
Histone deacetylase 3 as a novel therapeutic target in multiple myeloma
Minami, J., Suzuki, R., Mazitschek, R., Gorgun, G., Ghosh, B., Cirstea, D., Hu, Y., Mimura, N., Ohguchi, H., Cottini, F., et al. (2014) · Leukemia · doi:10.1038/leu.2013.231 · Cited by 159 · cited 1×
“A study conducted by Ho et al [25] suggested that the HDAC3 inhibitor overcomes the anti-apoptotic effect of BMSCs to multiple myeloma cells.”
Osteosarcoma: Molecular Pathogenesis and iPSC Modeling
Lin, Y.-H., Jewell, B. E., Gingold, J., Lu, L., Zhao, R., Wang, L. L., & Lee, D.-F. (2017) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2017.06.004 · Cited by 150
An, J., Ren, S., Murphy, S. J., Dalangood, S., Chang, C., Pang, X., Cui, Y., Wang, L., Pan, Y., Zhang, X., et al. (2015) · Molecular Cell · doi:10.1016/j.molcel.2015.07.025 · Cited by 149
Fu, J., Rodova, M., Roy, S. K., Sharma, J. P., Singh, K. P., Srivastava, R. K., & Shankar, S. (2012) · Cancer Letters · doi:10.1016/j.canlet.2012.11.018 · Cited by 148
Verrecchia, F., & Rédiní, F. (2018) · Frontiers in Oncology · doi:10.3389/fonc.2018.00133 · Cited by 135
Zhou, Q., Chen, F., Zhao, J., Li, B., Liang, Y., Pan, W., Zhang, S., Wang, X., & Zheng, D. (2016) · Oncotarget · doi:10.18632/oncotarget.13012 · Cited by 130
Zheng, Y., Wang, G., Chen, R., Hua, Y., & Cai, Z. (2018) · Stem Cell Research & Therapy · doi:10.1186/s13287-018-0780-x · Cited by 127
He, F., Song, Z., Chen, H., Chen, Z., Yang, P., Li, W., Yang, Z. H., Zhang, T., Wang, F., Wei, J., et al. (2018) · Oncogene · doi:10.1038/s41388-018-0432-8 · Cited by 114
Yu, Y., Zhang, M., Liu, J., Xu, B., Yang, J., Wang, N., Yan, S., Wang, F., He, X., Ji, G., et al. (2018) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2018.10.001 · Cited by 81
Fontanella, R., Pelagalli, A., Nardelli, A., D'Alterio, C., Ieranò, C., Cerchia, L., Lucarelli, E., Scala, S., & Zannetti, A. (2016) · Cancer Letters · doi:10.1016/j.canlet.2015.10.018 · Cited by 79
Ho, M., Chen, T., Liu, J., Dowling, P., Hideshima, T., Zhang, L., Morelli, E., Camci‐Unal, G., Wu, X., Tai, Y., et al. (2019) · Leukemia · doi:10.1038/s41375-019-0493-x · Cited by 74
Adjuvant Biophysical Therapies in Osteosarcoma
Carina, V., Costa, V., Sartori, M., Bellavia, D., Luca, A. D., Raimondi, L., Fini, M., & Giavaresi, G. (2019) · Cancers · doi:10.3390/cancers11030348 · Cited by 54
ERG expression in chondrogenic bone and soft tissue tumours
Shon, W., Folpe, A. L., & Fritchie, K. (2014) · Journal of Clinical Pathology · doi:10.1136/jclinpath-2014-202601 · Cited by 52
Xiao, M., Feng, Y., Liu, C., & Zhang, Z. (2018) · Cell Proliferation · doi:10.1111/cpr.12519 · Cited by 40
Mohseny, A. B., Xiao, W., Carvalho, R., Spaink, H. P., Hogendoorn, P. C. W., & Cleton-Jansen, A. (2012) · The Journal of Pathology · doi:10.1002/path.3998 · Cited by 31
Yoon, N., Park, M. S., Shigemoto, T., Peltier, G. C., & Lee, R. H. (2016) · Cell Death and Disease · doi:10.1038/cddis.2016.90 · Cited by 29
Wang, X., Yang, Y., Hu, X., Kawazoe, N., Yang, Y., & Chen, G. (2016) · Analytical Sciences · doi:10.2116/analsci.32.1177 · Cited by 25
The Evolutionarily Conserved Cassette Exon 7b Drives ERG's Oncogenic Properties
Jumbe, S. L., Porazinski, S. R., Oltean, S., Mansell, J. P., Vahabi, B., Wilson, I. D., & Ladomery, M. (2018) · Translational Oncology · doi:10.1016/j.tranon.2018.09.001 · Cited by 9
Flagged first, then by citations
Cited by
Sorted by citations
Therapeutic roles of mesenchymal stem cell-derived extracellular vesicles in cancer
Weng, Z., Zhang, B., Wu, C., Yu, F., Han, B. R., Li, B., & Li, L. (2021) · Journal of Hematology & Oncology · doi:10.1186/s13045-021-01141-y · Cited by 420
Sheng, G., Gao, Y., Yong, Y., & Wu, H. (2021) · Frontiers in Oncology · doi:10.3389/fonc.2021.780264 · Cited by 215
Zhang, H., Wang, J., Ren, T., Huang, Y., Liang, X., Yu, Y., Wang, W., Niu, J., & Guo, W. (2020) · Cancer Letters · doi:10.1016/j.canlet.2020.07.008 · Cited by 136
Self-citationRegulation of bone homeostasis: signaling pathways and therapeutic targets
Wu, Z., Li, W., Jiang, K., Lin, Z., Qian, C., Wu, M., Xia, Y., Li, N., Zhang, H., Xiao, H.-X., et al. (2024) · MedComm · doi:10.1002/mco2.657 · Cited by 129
lncRNA PVT1: a novel oncogene in multiple cancers
Li, R., Wang, X., Zhu, C., & Wang, K. (2022) · Cellular & Molecular Biology Letters · doi:10.1186/s11658-022-00385-x · Cited by 85
Metadata
- Authors
- Zhao, W., Qin, P., Zhang, D., Cui, X., Gao, J., Yu, Z., Chai, Y., Wang, J., & Li, J.
- Affiliations
- First Affiliated Hospital of Zhengzhou University (CN)
- Corresponding author
- Li, Juan
- Venue
- Aging
- Publisher
- Impact Journals, LLC
- Published
- 2019-11-07
- Volume
- 11
- Issue
- 21
- Pages
- 9581-9596
- ISSN
- 1945-4589 (ISSN-L)
- PMC
- PMC6874467
- OpenAlex
- W2984884798
- Cited by
- 115
- References
- 42
- Citations by year
- 2026: 5 · 2025: 14 · 2024: 18 · 2023: 16 · 2022: 26 · 2021: 19 · 2020: 17
- FWCI
- 5.13
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by
- Locations
- Aging (publishedVersion)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Circular RNAs in diseases
- Keywords
- bone marrow mesenchymal stem cells, exosomes, osteosarcoma, ERG, miR-183-5p, tumor growth, metastasis, protein stabilization, ubiquitination, tumor microenvironment, cell proliferation, cell migration
- MeSH terms
- Transcriptional Regulator ERG, Animals, Bone Neoplasms, Humans, Mice, Inbred BALB C, Mice, Nude, Neoplasm Metastasis, Neoplasm Transplantation, Osteosarcoma, MicroRNAs, Cell Line, Tumor, Exosomes, Primary Cell Culture, RNA, Long Noncoding
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
