CoV-GLUE: A Web Application for Tracking SARS-CoV-2 Genomic Variation
Open accessAbstract
Summary CoV-GLUE is an online web application for the interpretation and analysis of SARS-CoV-2 virus genome sequences, with a focus on amino acid sequence variation. It is based on the GLUE data-centric bioinformatics environment and provides a browsable database of amino acid replacements and coding region indels that have been observed in sequences from the pandemic. Users may also analyse their own SARS-CoV-2 sequences by submitting them to the web application to receive an interactive report containing visualisations of phylogenetic classification and highlighting genomic variation of potentially high impact, for example linked to primer mismatches.Availability and implementation Available at http://cov-glue.cvr.gla.ac.uk. Implemented using GLUE, an open source framework for the development of virus sequence data resources. Contact josh.singer@glasgow.ac.uk
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Mutations at K417, E484, and N501 often reduce their activity, and these antibodies select K417N, E484K, and N501Y in yeast and VSV S libraries.
“They are frequently sensitive to mutation in these residues and select for K417N, E484K and N501Y mutations in SARS-CoV-2 S protein expression libraries in yeast and VSV 12,15,33.”
From mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants (2021) · cites this paper as [33]
The panel also included circulating variant residues K417N, Y453F, S477R, N501Y, and D614G, some linked to rapidly rising case counts.
“Additionally, K417N, Y453F, S477R, N501Y, and D614G represent circulating variants some of which have been associated with rapidly increasing case numbers14,15,19,33–35.”
From mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants (2021) · cites this paper as [33]
Class 1 antibodies are resisted by A475V; class 2 by E484K and Q493R; class 3 by R346S, N439K, and N440K.
“A475V is resistant to class 1 antibodies (structurally defined as described29); E484K and Q493R are resistant to class 2 antibodies7,8,12,13,29,30,32,33; while R346S, N439K, and N440K are resistant to class 3 antibodies7,8,12,13,29,33.”
From mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants (2021) · cites this paper as [33]
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- Authors
- Singer, J., Gifford, R., Cotten, M., & Robertson, D.
- Affiliations
- MRC University of Glasgow Centre for Virus Research (GB)
- Corresponding author
- Singer, Joshua
- Venue
- Preprints.org
- Publisher
- MDPI AG
- Published
- 2020-06-18
- OpenAlex
- W3035920796
- Cited by
- 167
- References
- 25
- Citations by year
- 2025: 5 · 2024: 10 · 2023: 16 · 2022: 30 · 2021: 85 · 2020: 21
- Type
- posted content
- Language
- en
- Open access
- green
- doi.org
- Locations
- Preprints.org (acceptedVersion, cc-by)
- Funders
- Wellcome Trust (220977/Z/20/Z)
- UK Research and Innovation (MC_PC_19027)
- Department of Health and Social Care
- Department for International Development
- Medical Research Council (MC_PC_19027, MC UU 12014/12, COVID-19)
- Topics
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 detection and testing
- Keywords
- SARS-CoV-2, genomic variation, amino acid substitutions, indels, viral genome sequence, phylogenetic classification, primer mismatch, web application, COVID-19 pandemic
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