Cited by 51Bone Tissue Engineering MaterialsElectrospun Nanofibers in Biomedical ApplicationsMedicinal Plant Pharmacodynamics Research

Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds for the Repair of Rabbit Tibia Bone Defects

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Zhao, Hongbin, Tang, Junjie, Zhou, Dong, Weng, Yiping, Qin, Wen, Liu, Chun, Lv, Songwei, Wang, Wei +1 more·2020·International Journal of Nanomedicine

Abstract

BACKGROUND: Electrospinning is a widely used technology that can produce scaffolds with high porosity and surface area for bone regeneration. However, the small pore sizes in electrospun scaffolds constrain cell growth and tissue-ingrowth. In this study, novel drug-loading core-shell scaffolds were fabricated via electrospinning and freeze drying to facilitate the repair of tibia bone defects in rabbit models. MATERIALS AND METHODS: The collagen core scaffolds were freeze-dried containing icariin (ICA)-loaded chitosan microspheres. The shell scaffolds were electrospun using collagen, polycaprolactone and hydroxyapatite materials to form CPH composite scaffolds with the ones containing ICA microspheres named CPHI. The core-shell scaffolds were then cross-linked by genipin. The morphology, microstructure, physical and mechanical properties of the scaffolds were assessed. Rat marrow mesenchymal stem cells from the wistar rat were cultured with the scaffolds. The cell adhesion and proliferation were analysed. Adult rabbit models with tibial plateau defects were used to evaluate the performance of these scaffolds in repairing the bone defects over 4 to 12 weeks. RESULTS: The results reveal that the novel drug-loading core-shell scaffolds were successfully fabricated, which showed good physical and chemical properties and appropriate mechanical properties. Furthermore, excellent cells attachment was observed on the CPHI scaffolds. The results from radiography, micro-computed tomography, histological and immunohistochemical analysis demonstrated that abundant new bones were formed on the CPHI scaffolds. CONCLUSION: These new core-shell composite scaffolds have great potential for bone tissue engineering applications and may lead to effective bone regeneration and repair.

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  • Fabricated scaffolds showed enhanced physical, chemical, and mechanical properties.

    “Fabricated scaffolds showed [100] | enhanced physical, chemical, and | mechanical properties. Excellent cell | attachment was found on the | scaffold surface with growth of new | bones abundantly.”

    From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [100]

    The Results’ Scaffold Characterization section (page not available) reports greater mechanical strength and slower degradation after cross-linking, supporting enhanced mechanical and some physical properties. It confirms a chemical change through FTIR, but does not establish that the scaffolds’ chemical properties were enhanced. (LLM, conf=0.84)

    Experimental — accuracy not yet measured
  • Excellent cell attachment was found on the scaffold surface with growth of new bones abundantly.

    “Fabricated scaffolds showed [100] | enhanced physical, chemical, and | mechanical properties. Excellent cell | attachment was found on the | scaffold surface with growth of new | bones abundantly.”

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Authors
Zhao, H., Tang, J., Zhou, D., Weng, Y., Qin, W., Liu, C., Lv, S., Wang, W., & Zhao, X.
Affiliations
  • Changzhou No.2 People's Hospital (CN)
  • Changzhou University (CN)
  • Hexi University (CN)
Corresponding author
Zhao, Hongbin
Venue
International Journal of Nanomedicine
Published
2020-05
Volume
Volume 15
Pages
3039-3056
ISSN
  • 1176-9114 (ISSN-L)
  • 1178-2013
OpenAlex
W3022311892
Cited by
51
References
78
Citations by year
2026: 3 · 2025: 7 · 2024: 10 · 2023: 11 · 2022: 11 · 2021: 5 · 2020: 2 · 2019: 2
FWCI
2.28
Type
journal article
Language
en
Open access
Locations
  • International Journal of Nanomedicine (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • White Rose Research Online (University of Leeds, The University of Sheffield, University of York) (acceptedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Dove Medical Press (Taylor and Francis Group) (submittedVersion, cc-by-nc)
Funders
  • Government of Jiangsu Province (BE2018644)
  • Nanjing Medical University
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Medicinal Plant Pharmacodynamics Research
Keywords
icariin, electrospinning, freeze-drying, chitosan microspheres, genipin crosslinking, bone tissue engineering, bone defect repair, mesenchymal stem cells, drug-loaded scaffolds, rabbit model
MeSH terms
Animals, Bone Regeneration, Collagen, Flavonoids, Male, Materials Testing, Microspheres, Polyesters, Rabbits, Tibia, Porosity, Rats, Wistar, Durapatite, Tissue Engineering, Chitosan, Tissue Scaffolds, X-Ray Microtomography, Mesenchymal Stem Cells

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