MR imaging and targeting of a specific alveolar macrophage subpopulation in LPS-induced COPD animal model using antibody-conjugated magnetic nanoparticles
Open accessAbstract
PURPOSE: Targeting and noninvasive imaging of a specific alveolar macrophage subpopulation in the lung has revealed the importance for early and better diagnosis and therapy of chronic obstructive pulmonary disease (COPD). In this study, the in vivo effect of pulmonary administration of iron oxide nanoparticles on the polarization profile of macrophages was assessed, and a noninvasive free-breathing magnetic resonance imaging (MRI) protocol coupled with the use of biocompatible antibody-conjugated superparamagnetic iron oxide (SPIO) nanoparticles was developed to enable specific targeting and imaging of a particular macrophage subpopulation in lipopolysaccharide-induced COPD mice model. MATERIALS AND METHODS: Enzyme-linked immunosorbent assay, Real-time polymerase chain reaction, and flow cytometry analysis were performed to assess the biocompatibility of PEGylated dextran-coated SPIO nanoparticles. Specific biomarkers for M1 and M2 macrophages subsets were selected for conjugation with magnetic nanoparticles. MRI protocol using ultra-short echo time sequence was optimized to enable simultaneous detection of inflammation progress in the lung and detection of macrophages subsets. Flow cytometry and immunohistochemistry analysis were finally performed to confirm MRI readouts and to characterize the polarization profile of targeted macrophages. RESULTS: The tested SPIO nanoparticles, under the current experimental conditions, were found to be biocompatible for lung administration in preclinical settings. Cluster of differentiation (CD)86- and CD206-conjugated magnetic nanoparticles enabled successful noninvasive detection of M1 and M2 macrophage subpopulations, respectively, and were found to co-localize with inflammatory regions induced by lipopolysaccharide challenge. No variation in the polarization profile of targeted macrophages was observed, even though a continuum switch in their polarization might occur. However, further confirmatory studies are required to conclusively establish this observation. CONCLUSION: Coupling of magnetic iron oxide nanoparticles with a specific antibody targeted to a particular macrophage subpopulation could offer a promising strategy for an early and better diagnosis of pulmonary inflammatory diseases using noninvasive MRI.
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Noninvasive nanoparticle-mediated high-contrast imaging methods may be promising for diagnosis of pulmonary inflammation.
“Such noninvasive nanoparticle-mediated high-contrast imaging methods may be promising for the diagnosis of pulmonary inflammation.189”
From Targeted therapy in chronic diseases using nanomaterial-based drug delivery vehicles (2019) · cites this paper as [189]
Anti-CD86 and anti-CD206 antibody-conjugated SPIO NPs demonstrated specific adherence to proinflammatory M1 and resolutive M2 macrophage subpopulations in COPD mouse models.
“Anti-CD86 and anti-CD206 antibody-conjugated SPIO NPs were instilled in COPD mouse models via the intrapulmonary route, and the NPs demonstrated specific adherence to the proinflammatory (M1) and resolutive (M2) macrophage subpopulations.”
From Targeted therapy in chronic diseases using nanomaterial-based drug delivery vehicles (2019) · cites this paper as [189]
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Cites
Alternative Activation of Macrophages: An Immunologic Functional Perspective
Martinez, F. O., Helming, L., & Gordon, S. (2009) · Annual Review of Immunology · doi:10.1146/annurev.immunol.021908.132532 · Cited by 2,745 · see citing works
Rohrer, M., Bauer, H., Mintorovitch, J., Requardt, M., & Weinmann, H. (2005) · Investigative Radiology · doi:10.1097/01.rli.0000184756.66360.d3 · Cited by 1,681 · see citing works
Weissleder, R. (2006) · Science · doi:10.1126/science.1125949 · Cited by 1,107 · see citing works
Multifunctional magnetic nanoparticles for targeted imaging and therapy
MCCARTHY, J., & WEISSLEDER, R. (2008) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2008.03.014 · Cited by 892 · see citing works
Superparamagnetic iron oxide based MRI contrast agents: Current status of clinical application.
Wáng, Y. X. J. (2011) · PubMed Central · doi:10.3978/j.issn.2223-4292.2011.08.03 · Cited by 679 · see citing works
Tassa, C., Shaw, S. Y., & Weissleder, R. (2011) · Accounts of Chemical Research · doi:10.1021/ar200084x · Cited by 653 · see citing works
Molecular Imaging with Theranostic Nanoparticles
Jokerst, J. V., & Gambhir, S. S. (2011) · Accounts of Chemical Research · doi:10.1021/ar200106e · Cited by 554 · see citing works
Animal models of chronic obstructive pulmonary disease
Wright, J. L., Cosío, M. G., & Churg, A. (2008) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.90200.2008 · Cited by 451 · see citing works
Coating Optimization of Superparamagnetic Iron Oxide Nanoparticles for High T 2 Relaxivity
Tong, S., Hou, S., Zheng, Z., Zhou, J., & Bao, G. (2010) · Nano Letters · doi:10.1021/nl102623x · Cited by 423 · see citing works
The Cytokine Network in Chronic Obstructive Pulmonary Disease
Barnes, P. J. (2009) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/rcmb.2009-0220tr · Cited by 412 · see citing works
Alveolar Macrophages as Orchestrators of COPD
Barnes, P. J. (2004) · COPD Journal of Chronic Obstructive Pulmonary Disease · doi:10.1081/copd-120028701 · Cited by 361 · see citing works
Acute Lung Injury: How Macrophages Orchestrate Resolution of Inflammation and Tissue Repair
Herold, S., Mayer, K., & Lohmeyer, J. (2011) · Frontiers in Immunology · doi:10.3389/fimmu.2011.00065 · Cited by 331 · see citing works
Pulmonary applications and toxicity of engineered nanoparticles
Card, J. W., Zeldin, D. C., Bonner, J. C., & Nestmann, E. R. (2008) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00041.2008 · Cited by 308 · see citing works
Magnetic resonance imaging with ultrashort TE (UTE) PULSE sequences: Technical considerations
Tyler, D. J., Robson, M. D., Henkelman, R. M., YOUNG, I. R., & Bydder, G. M. (2007) · Journal of Magnetic Resonance Imaging · doi:10.1002/jmri.20851 · Cited by 223 · see citing works
Chitosan nanoparticles are compatible with respiratory epithelial cells in vitro
Grenha, A., Grainger, C. I., Dailey, L. A., Seijo, B., Martin, G. P., Remuñán‐López, C., & Forbes, B. (2007) · European Journal of Pharmaceutical Sciences · doi:10.1016/j.ejps.2007.02.008 · Cited by 222 · see citing works
Huang, J., Zhong, X., Wang, L., Yang, L. P. H., & Mao, H. Z. (2012) · Theranostics · doi:10.7150/thno.4006 · Cited by 217 · see citing works
Shapiro, S. D. (2000) · CHEST Journal · doi:10.1378/chest.117.5_suppl_1.223s · Cited by 153 · see citing works
Molecular and Cellular MR Imaging
Modo, M. (2007) · CRC Press eBooks · doi:10.1201/9781420004090 · Cited by 135 · see citing works
Duan, M., Li, W. C., Vlahos, R., Maxwell, M. J., Anderson, G. P., & Hibbs, M. L. (2012) · The Journal of Immunology · doi:10.4049/jimmunol.1200660 · Cited by 133 · see citing works
In Vivo Mononuclear Cell Tracking Using Superparamagnetic Particles of Iron Oxide
Richards, J. M. J., Shaw, C. A., Lang, N. N., Williams, M. C., Semple, S. I. K., MacGillivray, T., Gray, C. D., Crawford, J. H., Alam, S. R., Atkinson, A. P. M., et al. (2012) · Circulation Cardiovascular Imaging · doi:10.1161/circimaging.112.972596 · Cited by 114 · see citing works
Ultrashort echo time (UTE) MRI of the lung: Assessment of tissue density in the lung parenchyma
Togao, O., Tsuji, R., Ohno, Y., Dimitrov, I. E., & Takahashi, M. (2010) · Magnetic Resonance in Medicine · doi:10.1002/mrm.22521 · Cited by 108 · see citing works
Pusic, K. M., Aguilar, Z. P., McLoughlin, J., Kobuch, S., Xu, H. T., Tsang, M., Wang, A., & Hui, G. S. N. (2012) · The FASEB Journal · doi:10.1096/fj.12-218362 · Cited by 93 · see citing works
The role of macrophages in healing the wounded lung
Alber, A., Howie, S., Wallace, W. A., & Hirani, N. (2012) · International Journal of Experimental Pathology · doi:10.1111/j.1365-2613.2012.00833.x · Cited by 76 · see citing works
Beckmann, N., Cannet, C., Babin, A. L., Blé, F., Zurbruegg, S., Kneuer, R., & Dousset, V. (2009) · Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology · doi:10.1002/wnan.16 · Cited by 73 · see citing works
In Vivo Cellular Imaging for Translational Medical Research
Arbab, A. S., Janic, B., Haller, J., Pawelczyk, E., Liu, W. X., & Frank, J. A. (2009) · Current Medical Imaging Formerly Current Medical Imaging Reviews · doi:10.2174/157340509787354697 · Cited by 71 · see citing works
Altered lung function relates to inflammation in an acute LPS mouse model
Håkansson, H. F., Smailagic, A., Brunmark, C., Miller‐Larsson, A., & Lal, H. (2012) · Pulmonary Pharmacology & Therapeutics · doi:10.1016/j.pupt.2012.08.001 · Cited by 68 · see citing works
MRI of lung parenchyma in rats and mice using a gradient‐echo sequence
Beckmann, N., Tigani, B., Mazzoni, L., et al. (2001) · NMR in Biomedicine · doi:10.1002/nbm.706 · Cited by 64 · see citing works
Lipopolysaccharide Challenge of Humans as a Model for Chronic Obstructive Lung Disease Exacerbations
Kharitonov, S. A., & bring, U. S. O. (2007) · KARGER eBooks · doi:10.1159/000107056 · Cited by 56 · see citing works
Noninvasive detection of endotoxin-induced mucus hypersecretion in rat lung by MRI
Beckmann, N., Tigani, B., Sugar, R., et al. (2002) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00373.2001 · Cited by 46 · see citing works
Potential Use of Nanoparticle Based Contrast Agents in MRI: A Molecular Imaging Perspective
Oghabian, M. A., & Farahbakhsh, N. M. (2010) · Journal of Biomedical Nanotechnology · doi:10.1166/jbn.2010.1119 · Cited by 45 · see citing works
IL-17A and IL-17F Expression in B Lymphocytes
Vazquez‐Tello, A., Halwani, R., Li, R., Nadigel, J., Bar-Or, A., Mazer, B. D., Eidelman, D. H., Al‐Muhsen, S., & Hamid, Q. A. (2011) · International Archives of Allergy and Immunology · doi:10.1159/000329527 · Cited by 41 · see citing works
Self-citationConti, G., Tambalo, S., Villetti, G., et al. (2010) · Magnetic Resonance Materials in Physics Biology and Medicine · doi:10.1007/s10334-010-0203-1 · Cited by 39 · see citing works
Variation of lipopolysaccharide-induced acute lung injury in eight strains of mice
Alm, A.-S., Li, K., Chen, H., et al. (2010) · Respiratory Physiology & Neurobiology · doi:10.1016/j.resp.2010.02.009 · Cited by 37 · see citing works
Korsgren, M., Linden, M., Entwistle, N., et al. (2011) · Clinical Physiology and Functional Imaging · doi:10.1111/j.1475-097x.2011.01058.x · Cited by 34 · see citing works
Smith, I. W. G., & Schallenberg, M. (2012) · Agriculture Ecosystems & Environment · doi:10.1016/j.agee.2012.09.002 · Cited by 34 · see citing works
Faraj, A. A., Bessaad, A., Cieślar, K., et al. (2010) · Nanotechnology · doi:10.1088/0957-4484/21/17/175103 · Cited by 30 · see citing works
Longitudinal and noninvasive assessment of emphysema evolution in a murine model using proton MRI
Zurek, M., Boyer, L., Caramelle, P., et al. (2011) · Magnetic Resonance in Medicine · doi:10.1002/mrm.23281 · Cited by 30 · see citing works
Faraj, A. A., Luciani, N., Kolosnjaj‐Tabi, J., Mattar, E. H., Clémеnt, O., Wilhelm, C., & Gazeau, F. (2012) · Contrast Media & Molecular Imaging · doi:10.1002/cmmi.1516 · Cited by 29 · see citing works
Shahbazi‐Gahrouei, D., Abdolahi, M., Zarkesh‐Esfahani, S. H., et al. (2012) · BioMed Research International · doi:10.1155/2013/349408 · Cited by 28 · see citing works
1H and hyperpolarized 3He MR imaging of mouse with LPS‐induced inflammation
Olsson, L. E., Smailagic, A., Önnervik, P., et al. (2009) · Journal of Magnetic Resonance Imaging · doi:10.1002/jmri.21728 · Cited by 23 · see citing works
Rimkus, G., Bremer-Streck, S., Grüttner, C., et al. (2011) · Contrast Media & Molecular Imaging · doi:10.1002/cmmi.405 · Cited by 21 · see citing works
Molecular and Cellular MR Imaging
Modo, M. M. J., Bulte, J. W. M., & Kim, E. E. (2007) · Journal of Nuclear Medicine · doi:10.2967/jnumed.107.045369 · Cited by 19 · see citing works
Faraj, A. A. (2013) · Journal of Nanoparticle Research · doi:10.1007/s11051-013-1797-9 · Cited by 18 · see citing works
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Targeted therapy in chronic diseases using nanomaterial-based drug delivery vehicles
Singh, A. P., Biswas, A., Shukla, A., & Maiti, P. (2019) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-019-0068-3 · Cited by 645 · see citing works
Jin, R., Lin, B., Li, D., & Ai, H. (2014) · Current Opinion in Pharmacology · doi:10.1016/j.coph.2014.08.002 · Cited by 305 · see citing works
- Authors
- Al Faraj, A., Sultana Shaik, A., Afzal, S., Al Sayed, B., & Halwani, R.
- Affiliations
- King Saud University (SA)
- Venue
- International Journal of Nanomedicine
- Publisher
- Informa UK Limited
- Published
- 2014-03
- Volume
- 9
- Pages
- 1491
- ISSN
- 1176-9114 (ISSN-L)
- 1178-2013
- PMC
- PMC3969341
- OpenAlex
- W2088047310
- Cited by
- 83
- References
- 43
- Citations by year
- 2026: 6 · 2025: 8 · 2024: 4 · 2023: 6 · 2022: 8 · 2021: 8 · 2020: 7 · 2019: 6 · 2018: 3 · 2017: 6 · 2016: 5 · 2015: 10 · 2014: 6
- FWCI
- 4.67
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- International Journal of Nanomedicine (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Dove Medical Press (Taylor and Francis Group) (submittedVersion, cc-by-nc)
- Funders
- King Saud University
- Université de Lyon
- National Plan for Science, Technology and Innovation
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Characterization and Applications of Magnetic Nanoparticles
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Chronic Obstructive Pulmonary Disease (COPD) Research
- Keywords
- chronic obstructive pulmonary disease, alveolar macrophages, macrophage polarization, M1/M2 macrophages, superparamagnetic iron oxide nanoparticles, antibody-conjugated nanoparticles, lipopolysaccharide-induced lung inflammation, CD86, CD206, nanoparticle biocompatibility, ultrashort echo time MRI
- MeSH terms
- Animals, Antibodies, Contrast Media, Dextrans, Female, Lipopolysaccharides, Macrophages, Magnetic Resonance Imaging, Mice, Inbred BALB C, Pulmonary Alveoli, Sensitivity and Specificity, Reproducibility of Results, Pulmonary Disease, Chronic Obstructive, Mice, Magnetite Nanoparticles, Cell Tracking
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