Development of a Screening Assay for Ligands to the Estrogen Receptor Based on Magnetic Microparticles and LC-MS

van Breemen, Richard; Choi, Yongsoo

doi:10.2174/138620708783398340

Cited by 49Estrogen and related hormone effectsAnalytical chemistry methods developmentPhytoestrogen effects and research

Development of a Screening Assay for Ligands to the Estrogen Receptor Based on Magnetic Microparticles and LC-MS

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van Breemen, Richard, Choi, Yongsoo·2008·Combinatorial Chemistry & High Throughput Screening

DOI 10.2174/138620708783398340PMC PMC2736128

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Abstract

A high throughput screening assay for the identification of ligands to pharmacologically significant receptors was developed based on magnetic particles containing immobilized receptors followed by liquid chromatography-mass spectrometry (LC-MS). This assay is suitable for the screening of complex mixtures such as botanical extracts. For proof-of-principle, estrogen receptor-alpha (ER-alpha) and ER-beta were immobilized on magnetic particles functionalized with aldehyde or carboxylic acid groups. Alternatively, biotinylated ER was immobilized onto streptavidin-derivatized magnetic particles. The ER that was immobilized using the streptavidin-biotin chemistry showed higher activity than that immobilized on aldehyde or carboxylic acid functionalized magnetic particles. Immobilized ER was incubated with extracts of Trifolium pratense L. (red clover) or Humulus lupulus L. (hops). As a control for non-specific binding, each botanical extract was incubated with magnetic particles containing no ER. After magnetic separation of the particles containing bound ligands from the unbound components in the extract, the particles were washed, ligands were released using methanol, and then the ligands were identified using LC-MS. The estrogens genistein and daidzein were identified in the red clover extract, and the estrogen 8-prenylnaringenin was identified in the hop extract. These screening results are consistent with those obtained using previous screening approaches.

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Native MS detects ligands directly bound to a protein, whereas affinity MS approaches typically measure ligand binding indirectly as free compounds.
“An important difference between native MS and affinity MS is that native MS detects ligands directly bound to a protein, whereas affinity MS approaches typically measure ligand binding indirectly as free compounds.”
From Native metabolomics identifies the rivulariapeptolide family of protease inhibitors (2022) · cites this paper as [22]
The Introduction and Results and Discussion describe isolating estrogen receptor–ligand complexes, releasing the ligands, and identifying those released compounds by LC-MS. This supports the indirect-detection part for the paper’s assay, but the paper does not discuss native MS or establish that affinity MS approaches typically work this way. (LLM, conf=0.86)
Experimental — accuracy not yet measured
MagMASS is sterically restricted.
“However, a key limitation of the MagMASS method is the fact that it is sterically restricted.”
From Connecting metabolome and phenotype: recent advances in functional metabolomics tools for the identification of bioactive natural products (2024) · cites this paper as [78]
The Results and Discussion (page unavailable) report that high protein coverage on the magnetic particles caused loss of estrogen-receptor activity, supporting a limitation of the immobilization method. The paper does not identify steric restriction as the cause or describe MagMASS as sterically restricted. (LLM, conf=0.72)
Experimental — accuracy not yet measured

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Authors: van Breemen, R., & Choi, Y.
Affiliations: University of Illinois Chicago (US)
Venue: Combinatorial Chemistry & High Throughput Screening
Publisher: Bentham Science Publishers Ltd.
Published: 2008-01-01
Volume: 11
Issue: 1
Pages: 1-6
ISSN: 1386-2073 (ISSN-L)
DOI: 10.2174/138620708783398340
PMC: PMC2736128
OpenAlex: W2028396792
Cited by: 49
References: 0
Citations by year: 2025: 1 · 2024: 1 · 2023: 1 · 2022: 4 · 2021: 5 · 2020: 4 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 4 · 2015: 4 · 2014: 4 · 2013: 6 · 2012: 1
FWCI: 1.71
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Combinatorial Chemistry & High Throughput Screening (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Funders: Office of Dietary Supplements
National Institutes of Health
National Center for Complementary and Alternative Medicine
National Center for Complementary and Integrative Health (P50-AT00155, P50AT000155)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
Physical Sciences › Chemistry › Analytical Chemistry › Analytical chemistry methods development
Health Sciences › Medicine › Pathology and Forensic Medicine › Phytoestrogen effects and research
Keywords: estrogen receptor, estrogen receptor alpha, estrogen receptor beta, magnetic microparticles, immobilized receptors, high-throughput screening, ligand screening, plant extracts, red clover, Humulus lupulus, genistein, daidzein, 8-prenylnaringenin, streptavidin-biotin immobilization, magnetic separation
MeSH terms: Methanol, Binding Sites, Biological Assay, Chromatography, Liquid, Isoflavones, Ligands, Magnetics, Plant Extracts, Receptors, Estrogen, Mass Spectrometry, Time Factors, Genistein, Humulus, Trifolium, Flavanones, Estrogen Receptor alpha, Estrogen Receptor beta, Nanoparticles

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Development of a Screening Assay for Ligands to the Estrogen Receptor Based on Magnetic Microparticles and LC-MS

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