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Immobilization of Cholesterol Oxidase: An Overview

Ghosh, Shubhrima, Ahmad, Razi, Khare, Sunil Kumar·2018·The Open Biotechnology Journal
DOI 10.2174/1874070701812010176
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Abstract

Background: Cholesterol oxidases are bacterial oxidases widely used commercially for their application in the detection of cholesterol in blood serum, clinical or food samples. Additionally, these enzymes find potential applications as an insecticide, synthesis of anti-fungal antibiotics and a biocatalyst to transform a number of sterol and non-sterol compounds. However, the soluble form of cholesterol oxidases are found to be less stable when applied at higher temperatures, broader pH range, and incur higher costs. These disadvantages can be overcome by immobilization on carrier matrices. Methods: This review focuses on the immobilization of cholesterol oxidases on various macro/micro matrices as well as nanoparticles and their potential applications. Selection of appropriate support matrix in enzyme immobilization is of extreme importance. Recently, nanomaterials have been used as a matrix for immobilization of enzyme due to their large surface area and small size. The bio-compatible length scales and surface chemistry of nanoparticles provide reusability, stability and enhanced performance characteristics for the enzyme-nanoconjugates. Conclusion: In this review, immobilization of cholesterol oxidase on nanomaterials and other matrices are discussed. Immobilization on nanomatrices has been observed to increase the stability and activity of enzymes. This enhances the applicability of cholesterol oxidases for various industrial and clinical applications such as in biosensors.

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Cited by 26

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Metadata

Authors
Ghosh, S., Ahmad, R., & Khare, S. K.
Venue
The Open Biotechnology Journal
Publisher
Bentham Science Publishers Ltd.
Published
2018-08-30
Volume
12
Issue
1
Pages
176-188
ISSN
1874-0707
DOI
10.2174/1874070701812010176
Cited by
26
Language
en
Licence
creativecommons.org/licenses/by/4.0/legalcode (unspecified)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Immobilization of Cholesterol Oxidase: An Overview. https://app.cochat.ai/papers/10.2174/1874070701812010176
The paper
Ghosh, S., Ahmad, R., & Khare, S. K. (2018). Immobilization of Cholesterol Oxidase: An Overview. The Open Biotechnology Journal. https://doi.org/10.2174/1874070701812010176

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.