The DOI matches its Crossref record; 75 of the 75 references it cites were found in a registry or an index.
HTRF: A Technology Tailored for Drug Discovery - A Review of Theoretical Aspects and Recent Applications
Open accessAbstract
HTRF (Homogeneous Time Resolved Fluorescence) is the most frequently used generic assay technology to measure analytes in a homogenous format, which is the ideal platform used for drug target studies in high-throughput screening (HTS). This technology combines fluorescence resonance energy transfer technology (FRET) with time-resolved measurement (TR). In TR-FRET assays, a signal is generated through fluorescent resonance energy transfer between a donor and an acceptor molecule when in close proximity to each other. Buffer and media interference is dramatically reduced by dual-wavelength detection, and the final signal is proportional to the extent of product formation. The HTRF assay is usually sensitive and robust that can be miniaturized into the 384 and 1536-well plate formats. This assay technology has been applied to many antibody-based assays including GPCR signaling (cAMP and IP-One), kinases, cytokines and biomarkers, bioprocess (antibody and protein production), as well as the assays for protein-protein, proteinpeptide, and protein-DNA/RNA interactions.Since its introduction to the drug-screening world over ten years ago, researchers have used HTRF to expedite the study of GPCRs, kinases, new biomarkers, protein-protein interactions, and other targets of interest. HTRF has also been utilized as an alternative method for bioprocess monitoring. The first-generation HTRF technology, which uses Europium cryptate as a fluorescence donor to monitor reactions between biomolecules, was extended in 2008 through the introduction of a second-generation donor, Terbium cryptate (Tb), enhancing screening performance. Terbium cryptate possesses different photophysical properties compared to Europium, including increased quantum yield and a higher molar extinction coefficient. In addition to being compatible with the same acceptor fluorophors used with Europium, it can serve as a donor fluorophore to green-emitting fluors because it has multiple emission peaks including one at 490 nm. Moreover, all Terbium HTRF assays can be read on the same HTRF-compatible instruments as Europium HTRF assays.Overall, HTRF is a highly sensitive, robust technology for the detection of molecular interactions in vitro and is widely used for primary and secondary screening phases of drug development. This review addresses the general principles of HTRF and its current applications in drug discovery.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Blume-Jensen, P., & Hunter, T. (2001) · Nature · doi:10.1038/35077225 · Cited by 3,738
PROTEIN SERINE/THREONINE KINASES
Edelman, A. M., Blumenthal, D., & Krebs, E. G. (1987) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.56.070187.003031 · Cited by 1,435
SIGNALLING BY RECEPTOR TYROSINE KINASES
Fantl, W. J., Johnson, D. E., & Williams, L. T. (1993) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.62.1.453 · Cited by 1,118
Ciruela, F., Casadó, V., Rodrigues, R. J., Luján, R., Burgueño, J., Canals, M., Borycz, J., Rebola, N., Goldberg, S. R., Mallol, J., et al. (2006) · Journal of Neuroscience · doi:10.1523/jneurosci.3574-05.2006 · Cited by 591
Principles and Biophysical Applications of Lanthanide-Based Probes
Selvin, P. R. (2002) · Annual Review of Biophysics and Biomolecular Structure · doi:10.1146/annurev.biophys.31.101101.140927 · Cited by 549
Maurel, D., Comps‐Agrar, L., Brock, C., Rives, M., Bourrier, E., Ayoub, M. A., Bazin, H., Tinel, N., Durroux, T., Prézeau, L., et al. (2008) · Nature Methods · doi:10.1038/nmeth.1213 · Cited by 500
Rare earth cryptates and homogeneous fluoroimmunoassays with human sera
Mathis, G. (1993) · Clinical Chemistry · doi:10.1093/clinchem/39.9.1953 · Cited by 393
Mathis, G. (1995) · Clinical Chemistry · doi:10.1093/clinchem/41.9.1391 · Cited by 339
Levoye, A., Dam, J., Ayoub, M. A., Guillaume, J., Couturier, C., Delagrange, P., & Jockers, R. (2006) · The EMBO Journal · doi:10.1038/sj.emboj.7601193 · Cited by 335
Dopamine D2 receptors form higher order oligomers at physiological expression levels
Guo, W. X., Urizar, E., Králíková, M., Mobarec, J. C., Shi, L., Filizola, M., & Javitch, J. A. (2008) · The EMBO Journal · doi:10.1038/emboj.2008.153 · Cited by 334
ANTENNA EFFECT IN LUMINESCENT LANTHANIDE CRYPTATES: A PHOTOPHYSICAL STUDY
Alpha‐Bazin, B., Ballardini, R., Balzani, V., Lehn, J., Perathoner, S., & Sabbatini, N. (1990) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1990.tb04185.x · Cited by 278
GPR30 Does Not Mediate Estrogenic Responses in Reproductive Organs in Mice
Otto, C., Fuchs, I., Kauselmann, G., Kern, H., Zevnik, B., Andreasen, P., Schwarz, G., Altmann, H., Klewer, M., Schoor, M., et al. (2009) · Biology of Reproduction · doi:10.1095/biolreprod.108.071175 · Cited by 278
Jean, A., Conductier, G., Manrique, C., Bouras, C., Berta, P., Hen, R., Charnay, Y., Bockaert, J., & Compan, V. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0701471104 · Cited by 196
Chicoric acid, a new compound able to enhance insulin release and glucose uptake
Tousch, D., Lajoix, A., Hosy, E., Azay, J., Ferrare, K., Jahannault-Talignani, C., Cros, G. H., & Petit, P. (2008) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2008.09.088 · Cited by 177
Bazin, H., Trinquet, E., & Mathis, G. (2002) · Reviews in Molecular Biotechnology · doi:10.1016/s1389-0352(01)00040-x · Cited by 158
Lewis, H. D., Beher, D., Cookson, N., Oakley, A. E., Piggott, M. A., Morris, C. M., Jaros, E., Perry, R., Ince, P. G., Kenny, R. A., et al. (2006) · Neuropathology and Applied Neurobiology · doi:10.1111/j.1365-2990.2006.00696.x · Cited by 143
Bellon, S. F., Kaplan‐Lefko, P. J., Yang, Y., Zhang, Y., Moriguchi, J., Rex, K. L., Johnson, C. W., Rose, P., Long, A. M., O'Connor, A. B., et al. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m705774200 · Cited by 140
Trinquet, E., Fink, M., Bazin, H., Grillet, F., Maurin, F., Bourrier, E., Ansanay, H., Leroy, C., Michaud, A., Durroux, T., et al. (2006) · Analytical Biochemistry · doi:10.1016/j.ab.2006.08.002 · Cited by 135
Maurel, D., Kniazeff, J., Mathis, G., Trinquet, E., Pin, J., & Ansanay, H. (2004) · Analytical Biochemistry · doi:10.1016/j.ab.2004.02.013 · Cited by 126
Mathis, G. (1999) · SLAS DISCOVERY · doi:10.1177/108705719900400605 · Cited by 121
Activation of a Dimeric Metabotropic Glutamate Receptor by Intersubunit Rearrangement
Brock, C., Oueslati, N., Soler, S., Boudier, L., Rondard, P., & Pin, J. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m702542200 · Cited by 114
Osteoblast expression of an engineered G s -coupled receptor dramatically increases bone mass
Hsiao, E. C., Boudignon, B. M., Chang, W. C., Bencsik, M., Peng, J. C., Nguyen, T., Manalac, C., Halloran, B. P., Conklin, B. R., & Nissenson, R. A. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0707457105 · Cited by 111
Albizu, L., Balestre, M., Breton, C., Pin, J., Manning, M., Mouillac, B., Barberis, C., & Durroux, T. (2006) · Molecular Pharmacology · doi:10.1124/mol.106.025684 · Cited by 108
Homogeneous time-resolved fluorescence and its applications for kinase assays in drug discovery
Jia, Y., Quinn, C. M., Gagnon, A. I., & Talanian, R. V. (2006) · Analytical Biochemistry · doi:10.1016/j.ab.2006.05.006 · Cited by 102
Coupling of Agonist Binding to Effector Domain Activation in Metabotropic Glutamate-like Receptors
Rondard, P., Liu, J., Huang, S., Malhaire, F., Vol, C., Pinault, A., Labesse, G., & Pin, J. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m602277200 · Cited by 98
Ayoub, M. A., Maurel, D., Binet, V., Fink, M., Prézeau, L., Ansanay, H., & Pin, J. (2007) · Molecular Pharmacology · doi:10.1124/mol.106.030304 · Cited by 90
Yamamoto, W., Mikami, T., & Iwamura, H. (2008) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2008.01.010 · Cited by 89 · cited 1×
“The application of HTRF to functional detection of GPCRs has been illustrated for many receptors including 5HT4 and 5HT6 [9, 10], BLT1 and cysLT1 leukotriene receptors [11], canabinoid receptors [12, 13], H1, 2 and 3 receptors [14, 15], PTH receptor [16], PACAP and PAR1 [17, 18] and a number of orphan receptors such a…”
MethodFunctioning of the dimeric GABAB receptor extracellular domain revealed by glycan wedge scanning
Rondard, P., Huang, S., Monnier, C., Tu, H., Blanchard, B., Oueslati, N., Malhaire, F., Li, Y., Trinquet, E., Labesse, G., et al. (2008) · The EMBO Journal · doi:10.1038/emboj.2008.64 · Cited by 84
Peres, C. M., Aronoff, D. M., Serezani, C. H., Flamand, N., Faccioli, L. H., & Peters-Golden, M. L. (2007) · The Journal of Immunology · doi:10.4049/jimmunol.179.8.5454 · Cited by 75
Delcourt, N., Thouvenot, É., Chanrion, B., Galéotti, N., Jouin, P., Bockaert, J., & Marin, P. (2007) · The EMBO Journal · doi:10.1038/sj.emboj.7601608 · Cited by 69
Ling, S., Pi, X., & Holoshitz, J. (2007) · The Journal of Immunology · doi:10.4049/jimmunol.179.9.6359 · Cited by 69
Oligomerization of Recombinant and Endogenously Expressed Human Histamine H4 Receptors
van Rijn, R. M., Chazot, P. L., Shenton, F. C., Sansuk, K., Bakker, R. A., & Leurs, R. (2006) · Molecular Pharmacology · doi:10.1124/mol.105.020818 · Cited by 67
Dzyakanchuk, A. A., Balázs, Z., Nashev, L. G., Amrein, K. E., & Odermatt, A. (2008) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2008.08.009 · Cited by 62
Protein Serine Threonine Kinases
Edelman, A. (1987) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.56.1.567 · Cited by 60
The Lysosomal Transmembrane Protein 9B Regulates the Activity of Inflammatory Signaling Pathways
Dodeller, F., Gottar, M., Huesken, D., Iourgenko, V., & Cenni, B. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m801908200 · Cited by 59
Romero, G., Sánchez, E., Pujol, M., Pérez, P., Codony, X., Holenz, J., Buschmann, H., & Pauwels, P. J. (2006) · British Journal of Pharmacology · doi:10.1038/sj.bjp.0706827 · Cited by 57
Viral hijacking of human receptors through heterodimerization
Vischer, H. F., Nijmeijer, S., Smit, M. J., & Leurs, R. (2008) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2008.09.082 · Cited by 57
Functional Adhesiveness of the CX3CL1 Chemokine Requires Its Aggregation
Hermand, P., Pincet, F., Carvalho, S., Ansanay, H., Trinquet, E., Daoudi, M., Combadière, C., & Déterre, P. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m802638200 · Cited by 56
Inhibition of Early Steps of HIV-1 Replication by SNF5/Ini1
Maroun, M., Delelis, O., Coadou, G., Bader, T., Ségéral, E., Mbemba, G., Petit, C., Sonigo, P., Rain, J., Mouscadet, J. F., et al. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m604849200 · Cited by 52
Adams, J. W., Wang, J., Davis, J. R., Liaw, C. W., Gaidarov, I., Gatlin, J., Dalton, N. D., Gu, Y., Ross, J., Behan, D. P., et al. (2008) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00368.2008 · Cited by 51
Lemay, J., Maidou-Peindara, P., Bader, T., Ennifar, E., Rain, J., Bénarous, R., & Liu, L. X. (2008) · Retrovirology · doi:10.1186/1742-4690-5-47 · Cited by 45
Newton, P., Harrison, P., & Clulow, S. (2008) · SLAS DISCOVERY · doi:10.1177/1087057108321086 · Cited by 45
The ubiquitin E3 ligase POSH regulates calcium homeostasis through spatial control of Herp
Tuvia, S., Taglicht, D., Erez, O., Alroy, I., Alchanati, I., Bicoviski, V., Dori-Bachash, M., Ben-Avraham, D., & Reiss, Y. (2007) · The Journal of Cell Biology · doi:10.1083/jcb.200611036 · Cited by 44
Wang, J., & Norcross, M. A. (2008) · Drug Discovery Today · doi:10.1016/j.drudis.2008.04.004 · Cited by 43
A simple and rapid assay for heparanase activity using homogeneous time-resolved fluorescence
Enomoto, K., Okamoto, H., Numata, Y., & Takemoto, H. (2006) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/j.jpba.2006.01.032 · Cited by 42
Liu, K., Titus, S., Southall, N. T., Zhu, P., Inglese, J., Austin, C. P., & Zheng, W. (2008) · Current Chemical Genomics · doi:10.2174/1875397300801010070 · Cited by 42
Schröter, T., Minond, D., Weiser, A. M., Dao, C. T., Habel, J. E., Spicer, T., Chase, P. S., Baillargeon, P., Scampavia, L. D., Schürer, S. C., et al. (2008) · SLAS DISCOVERY · doi:10.1177/1087057107310806 · Cited by 42
Fluorinated pyrazole acids are agonists of the high affinity niacin receptor GPR109a
Skinner, P. J., Cherrier, M. C., Webb, P. J., Shin, Y., Gharbaoui, T., Lindstrom, A., Hong, V. T. M., Tamura, S. Y., Dang, H. T., Pride, C. C., et al. (2007) · Bioorganic & Medicinal Chemistry Letters · doi:10.1016/j.bmcl.2007.07.101 · Cited by 37
Novel non-steroidal inhibitors of human 11β-hydroxysteroid dehydrogenase type 1
Vicker, N., Su, X., Ganeshapillai, D., Smith, A., Purohit, A., Reed, M. J., & Potter, B. V. L. (2007) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2007.03.023 · Cited by 31
Bergsdorf, C., Kropp-Goerkis, C., Kaehler, I., Ketscher, L., Boemer, U., Parczyk, K., & Bader, B. (2008) · Assay and Drug Development Technologies · doi:10.1089/adt.2007.108 · Cited by 29
Gracias, V. J., Ji, Z., Akritopoulou‐Zanze, I., Abad‐Zapatero, C., Huth, J. R., Song, D., Hajduk, P. J., Johnson, E. F., Glaser, K. B., Marcotte, P. A., et al. (2008) · Bioorganic & Medicinal Chemistry Letters · doi:10.1016/j.bmcl.2008.03.021 · Cited by 29
Discovery of Selective Small-Molecule CD80 Inhibitors
Uvebrant, K., Thrige, D. D. G., Rosén, A., Åkesson, M., Berg, H., Walse, B., & Björk, P. (2007) · SLAS DISCOVERY · doi:10.1177/1087057107300464 · Cited by 29
Novel inhibitor for fibroblast growth factor receptor tyrosine kinase
Kammasud, N., Boonyarat, C., Tsunoda, S., Sakurai, H., Saiki, I., Grierson, D. S., & Vajragupta, O. (2007) · Bioorganic & Medicinal Chemistry Letters · doi:10.1016/j.bmcl.2007.06.058 · Cited by 28
Yabuki, N., Watanabe, S., Kudoh, T., NIHIRA, S.-I., & Miyamoto, C. (1999) · Combinatorial Chemistry & High Throughput Screening · doi:10.2174/1386207302666220204200224 · Cited by 27
Su, X., Vicker, N., Lawrence, H. R., Smith, A., Purohit, A., Reed, M. J., & Potter, B. V. L. (2007) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2007.03.019 · Cited by 25
Histamine Suppresses Fibulin-5 and Insulin-like Growth Factor-II Receptor Expression in Melanoma
Pós, Z., Wiener, Z., Pócza, P., Rácz, M., Tóth, S., Darvas, Z., Molnár, V., Hegyesi, H., & Falus, A. (2008) · Cancer Research · doi:10.1158/0008-5472.can-07-2816 · Cited by 23
Yabuki, N., Watanabe, S., Kudoh, T., et al. (1999) · PubMed · Cited by 22
Detailed pharmacological characterization of GT-2331 for the rat histamine H3 receptor
Ito, S., Yoshimoto, R., Miyamoto, Y., Mitobe, Y., Nakamura, T., Ishihara, A., MacNeil, D. J., Kanatani, A., & Tokita, S. (2005) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2005.10.066 · Cited by 22
Discovery of thienopyridines as Src-family selective Lck inhibitors
Abbott, L., Betschmann, P., Burchat, A. F., Calderwood, D. J., Davis, H., Hrnčiar, P., Hirst, G. C., Li, B., Morytko, M. J., Mullen, K. D., et al. (2006) · Bioorganic & Medicinal Chemistry Letters · doi:10.1016/j.bmcl.2006.12.035 · Cited by 22
Cascio, M. G., Bisogno, T., Palazzo, E., Thomas, A., van der Stelt, M., Brizzi, A., de Novellis, V., Marabese, I., Ross, R. A., van de Doelen, T., et al. (2006) · British Journal of Pharmacology · doi:10.1038/sj.bjp.0706888 · Cited by 22
Jia, Y., Quinn, C. M., Clabbers, A., Talanian, R. V., Xu, Y., Wishart, N., & Allen, H. (2005) · Analytical Biochemistry · doi:10.1016/j.ab.2005.11.010 · Cited by 21
Kumpošt, J., Syrová, Z., Kulihova, L., Franková, D., Bologna, J., Hlaváčková, V., Prézeau, L., Králíková, M., Hruskova, B., Pin, J., et al. (2008) · Neuropharmacology · doi:10.1016/j.neuropharm.2008.06.073 · Cited by 21
Current status of HTRF®technology in kinase assays
Jia, Y. (2008) · Expert Opinion on Drug Discovery · doi:10.1517/17460440802518171 · Cited by 19
Discovery of N-phenyl nicotinamides as potent inhibitors of Kdr
Dominguez, C., Smith, L. M., Huang, Q. Z., Yuan, C., Ouyang, X., Cai, L., Chen, P. L., Kim, J. Y., Harvey, T. S., Syed, R. S., et al. (2007) · Bioorganic & Medicinal Chemistry Letters · doi:10.1016/j.bmcl.2007.07.077 · Cited by 17
Leblanc, V., Delaunay, V., Lelong, J., Gas, F., Mathis, G., Grassi, J. J., & May, E. (2002) · Analytical Biochemistry · doi:10.1016/s0003-2697(02)00229-4 · Cited by 17
A homogeneous resonance energy transfer-based assay to monitor MutS/DNA interactions
Lopez‐Crapez, E., Malinge, J., Gatchitch, F., Casano, L., Langlois, T., Pugnière, M., Roquet, F., Mathis, G., & Bazin, H. (2008) · Analytical Biochemistry · doi:10.1016/j.ab.2008.09.004 · Cited by 16
Development of a HTRF® Kinase Assay for Determination of Syk Activity
Harbert, C., Marshall, J., Soh, S. C., & Steger, K. K. (2008) · Current Chemical Genomics · doi:10.2174/1875397300801010020 · Cited by 15
HTRF-Based Assay for Microsomal Prostaglandin E2 Synthase-1 Activity
Goedken, E. R., Gagnon, A. I., Overmeyer, G. T., Liu, J., Petrillo, R. A., Burchat, A. F., & Tomlinson, M. J. (2008) · SLAS DISCOVERY · doi:10.1177/1087057108321145 · Cited by 13
Wang, Y., Malkowski, M. G., Hailey, J., Turek-Etienne, T. C., Tripodi, T., & Pachter, J. A. (2004) · PubMed · Cited by 12
HTRF® KinEASE™ TK: a new solution for tyrosine kinase screening
Tardieu, J.-L. (2007) · Nature Methods · doi:10.1038/nmeth1082 · Cited by 11
Identifying Nonselective Hits from a Homogeneous Calcium Assay Screen
Cassutt, K. J., Orsini, M. J., Abousleiman, M., Colone, D., & Tang, W. (2007) · SLAS DISCOVERY · doi:10.1177/1087057106298538 · Cited by 11
Wang, D.-Y., Lu, Q., Walsh, S. L., Payne, L. A., Modha, S. S., Scott, M. J., Sweitzer, T. D., Ames, R. S., Krosky, D. J., & Li, H. (2008) · Molecular Biotechnology · doi:10.1007/s12033-008-9050-y · Cited by 10
Identification and characterization of small‐molecule inhibitors of Tie2 kinase
Liu, J., Lin, T. H., Cole, A. G., Wen, R., Zhao, L., Brescia, M., Jacob, B., Hussain, Z., Appell, K. C., Henderson, I. M., et al. (2008) · FEBS Letters · doi:10.1016/j.febslet.2008.02.003 · Cited by 8
Maurel, D. (2004) · Analytical Biochemistry · doi:10.1016/s0003-2697(04)00162-9 · Cited by 6
Cellular Ser/Thr-Kinase Assays Using Generic Peptide Substrates
Adams, D. G., Wang, Y., Mak, P. A., Chyba, J., Shalizi, O., Matzen, J., Anderson, P., Smith, T. R., Garcia, M. E., Welch, G. L., et al. (2008) · Current Chemical Genomics · doi:10.2174/1875397300801010054 · Cited by 5
Flagged first, then by citations
Research works printed but not linked: 1
- [23]Amoravain M Lyotard S Jaga D Lebreton ML Servent F Bomer U Introduction of a new HTRF acceptor, d2: Development of a complete GPCR platform for a Gs, Gi and Gq screening SBS 2005 11th Annual Conference Geneva
Cited by
Sorted by citations
Lanthanide Luminescence for Biomedical Analyses and Imaging
Bünzli, J. G. (2010) · Chemical Reviews · doi:10.1021/cr900362e · Cited by 2,699
Lanthanide NIR luminescence for telecommunications, bioanalyses and solar energy conversion
Bünzli, J. G., & Eliseeva, S. V. (2010) · Journal of Rare Earths · doi:10.1016/s1002-0721(09)60208-8 · Cited by 642
A stable trimeric influenza hemagglutinin stem as a broadly protective immunogen
Impagliazzo, A., Milder, F. J., Kuipers, H., Wagner, M. V., Zhu, X., Hoffman, R. M. B., van Meersbergen, R., Huizingh, J., Wanningen, P., Verspuij, J. W. A., et al. (2015) · Science · doi:10.1126/science.aac7263 · Cited by 591
SMN2 splicing modifiers improve motor function and longevity in mice with spinal muscular atrophy
Naryshkin, N. A., Weetall, M., Dakka, A., Narasimhan, J., Zhao, X., Feng, Z., Ling, K. K. Y., Karp, G. M., Qi, H., Woll, M. G., et al. (2014) · Science · doi:10.1126/science.1250127 · Cited by 524
Modulators of Protein–Protein Interactions
Milroy, L.-G., Grossmann, T. N., Hennig, S., Brunsveld, L., & Ottmann, C. (2014) · Chemical Reviews · doi:10.1021/cr400698c · Cited by 497
Metadata
- Authors
- Degorce, F.
- Venue
- Current Chemical Genomics
- Publisher
- Bentham Science Publishers Ltd.
- Published
- 2009-03-17
- Volume
- 3
- Issue
- 1
- Pages
- 22-32
- ISSN
- 1875-3973 (ISSN-L)
- PMC
- PMC2802762
- OpenAlex
- W2073113196
- Cited by
- 501
- References
- 74
- Citations by year
- 2026: 29 · 2025: 31 · 2024: 35 · 2023: 37 · 2022: 31 · 2021: 53 · 2020: 35 · 2019: 29 · 2018: 34 · 2017: 28 · 2016: 23 · 2015: 25 · 2014: 39 · 2013: 28 · 2012: 26
- FWCI
- 5.90
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- cc-by
- Locations
- Current Chemical Genomics (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- homogeneous time-resolved fluorescence, time-resolved fluorescence resonance energy transfer, drug discovery, high-throughput screening, fluorescence resonance energy transfer, europium cryptate, G protein-coupled receptor signaling, kinase activity assay, protein-protein interaction, bioprocess monitoring, antibody-based assays, biomarkers
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
