The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
High-Resolution DNA Melting Analysis for Simple and Efficient Molecular Diagnostics
Abstract
High-resolution melting of DNA is a simple solution for genotyping, mutation scanning and sequence matching. The melting profile of a PCR product depends on its GC content, length, sequence and heterozygosity and is best monitored with saturating dyes that fluoresce in the presence of double-stranded DNA. Genotyping of most variants is possible by the melting temperature of the PCR products, while all variants can be genotyped with unlabeled probes. Mutation scanning and sequence matching depend on sequence differences that result in heteroduplexes that change the shape of the melting curve. High-resolution DNA melting has several advantages over other genotyping and scanning methods, including an inexpensive closed tube format that is homogenous, accurate and rapid. Owing to its simplicity and speed, the method is a good fit for personalized medicine as a rapid, inexpensive method to predict therapeutic response.
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Product Differentiation by Analysis of DNA Melting Curves during the Polymerase Chain Reaction
Ririe, K. M., Rasmussen, R. P., & Wittwer, C. T. (1997) · Analytical Biochemistry · doi:10.1006/abio.1996.9916 · Cited by 1,654
Self-citationContinuous Fluorescence Monitoring of Rapid Cycle DNA Amplification
Wittwer, C. T., Herrmann, M. G., Moss, A. A., & Rasmussen, R. P. (2013) · BioTechniques · doi:10.2144/000114043 · Cited by 1,274
Self-citationHigh-Resolution Genotyping by Amplicon Melting Analysis Using LCGreen
Wittwer, C. T., Reed, G. H., Gundry, C. N., Vandersteen, J. G., & Pryor, R. J. (2003) · Clinical Chemistry · doi:10.1373/49.6.853 · Cited by 1,257
Self-citationContinuous Fluorescence Monitoring of Rapid Cycle DNA Amplification
Wittwer, C. T., Herrmann, M. G., Moss, A. A., & Rasmussen, R. P. (1997) · BioTechniques · doi:10.2144/97221bi01 · Cited by 1,143
Self-citationThe LightCycler TM : A Microvolume Multisample Fluorimeter with Rapid Temperature Control
Wittwer, C. T., Ririe, K. M., Andrew, R. V., David, D. A., Gundry, R. A., & Balis, U. J. (1997) · BioTechniques · doi:10.2144/97221pf02 · Cited by 893
Self-citationDenaturing high-performance liquid chromatography: A review
Xiao, W., & Oefner, P. J. (2001) · Human Mutation · doi:10.1002/humu.1130 · Cited by 725
Genetics and Genomics of Core Short Tandem Repeat Loci Used in Human Identity Testing
Butler, J. M. (2006) · Journal of Forensic Sciences · doi:10.1111/j.1556-4029.2006.00046.x · Cited by 686
Genotyping of Single-Nucleotide Polymorphisms by High-Resolution Melting of Small Amplicons
Liew, M., Pryor, R., Palais, R., Meadows, C., Erali, M., Lyon, E., & Wittwer, C. (2004) · Clinical Chemistry · doi:10.1373/clinchem.2004.032136 · Cited by 660
Self-citationWojdacz, T. K., & Dobrovic, A. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkm013 · Cited by 551
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The current understanding on the impact of KRAS on colorectal cancer
Meng, M., Zhong, K., Jiang, T., Liu, Z., Kwan, H. Y., & Su, T. (2021) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2021.111717 · Cited by 171
Schreiber, L., Nader-Nieto, A. C., Schönhals, E. M., Walkemeier, B., & Gebhardt, C. (2014) · G3 Genes|Genomes|Genetics · doi:10.1534/g3.114.012377 · Cited by 81
Point mutation frequency in the FMR1 gene as revealed by fragile X syndrome screening
Handt, M., Epplen, A., Hoffjan, S., Mese, K., Epplen, J. T., & Dekomien, G. (2014) · Molecular and Cellular Probes · doi:10.1016/j.mcp.2014.08.003 · Cited by 48
Metadata
- Authors
- Reed, G. H., Kent, J. O., & Wittwer, C. T.
- Venue
- Pharmacogenomics
- Publisher
- Informa UK Limited
- Published
- 2007-06
- Volume
- 8
- Issue
- 6
- Pages
- 597-608
- ISSN
- 1462-2416 (ISSN-L)
- 1744-8042
- OpenAlex
- W2112444375
- Cited by
- 678
- References
- 47
- FWCI
- 12.21
- Type
- journal article
- Language
- en
- Open access
- closed
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
