Cited by 16Muscle Physiology and DisordersFOXO transcription factor regulationExercise and Physiological Responses

Changes in FOXO and proinflammatory cytokines in the late stage of immobilized fast and slow muscle atrophy

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OKAMOTO, Takeshi, MACHIDA, Shuichi·2017·Biomedical Research

Abstract

The present study aimed to examine the changes in the expression of Forkhead box protein O (FOXO) and proinflammatory cytokines in both slow-twitch soleus and fast-twitch plantaris muscles following cast immobilization. Male C57BL/6 mice were subjected to cast immobilization for 7 and 21 days. Cast immobilization increased FOXO3a mRNA and total protein expression in both the soleus and plantaris muscles. Although FOXO3a phosphorylation tended to increase in response to cast immobilization in both muscles, a significant increase was evident after 21 days of immobilization only in the soleus muscle. The degree of the response of FOXO3a was very different between the soleus and plantaris muscles; however, the kinetics of FOXO3a in both muscles were similar. Thus, the regulation of muscle atrophy by FOXO might act via a common mechanism in both slow-twitch soleus and fast-twitch plantaris muscles. Gene expression of proinflammatory cytokines tended to increase in response to cast immobilization, and a significant increase was evident after 21 days of immobilization in the soleus muscle. However, in the plantaris muscle, proinflammatory cytokine gene expression remained unchanged throughout the immobilization period; nevertheless, immobilization induced greater reduction in muscle fiber cross-sectional area in the plantaris than in the soleus muscle. Thus, these observations indicate that regulation of muscle atrophy by proinflammatory cytokines might contribute to muscle fiber type-specific mechanisms.

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Crossref · 10.2220/biomedres.38.331

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  • Proinflammatory cytokines in muscle trigger NF-κB signaling pathways to mediate muscle degradation.

    “Moreover, proinflammatory cytokines present in the muscle trigger the nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) signaling pathways to mediate muscle degradation [8,9].”

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Authors
OKAMOTO, T., & MACHIDA, S.
Affiliations
  • Tokai University (JP)
  • Juntendo University (JP)
Venue
Biomedical Research
Published
2017
Volume
38
Issue
6
Pages
331-342
ISSN
  • 0388-6107 (ISSN-L)
  • 1880-313X
OpenAlex
W2771261915
Cited by
16
References
48
Citations by year
2023: 2 · 2021: 5 · 2020: 2 · 2019: 4 · 2018: 3
FWCI
1.00
Type
journal article
Language
en
Open access
Locations
  • Biomedical Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Ministry of Education, Culture, Sports, Science and Technology
  • Japan Society for the Promotion of Science (16K12940, 24300224, 16H03205, 18680048, 18200041)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › FOXO transcription factor regulation
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
Keywords
FOXO3, inflammatory cytokines, muscle atrophy, cast immobilization, soleus muscle, plantaris muscle, fast-twitch muscle, slow-twitch muscle, FOXO3a phosphorylation, muscle fiber cross-sectional area, skeletal muscle
MeSH terms
Animals, Immobilization, Immunohistochemistry, Male, Muscular Atrophy, Organ Size, RNA, Messenger, Time Factors, Biomarkers, Gene Expression, Cytokines, Muscle, Skeletal, Inflammation Mediators, Ubiquitin-Protein Ligases, Mice, Forkhead Transcription Factors

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