The Repair of DNA Damages Induced in Normal Human Skin Fibroblasts Exposed to Simulated Sunlight
Abstract
The induction and repair of DNA damages produced by exposure of normal human skin fibroblasts to the simulated sunlight produced by a solar simulator were examined. The photoproducts measured were pyrimidine dimers, E. coli endonuclease III-sensitive sites, 6-4 photoproducts, Dewar isomers, DNA-protein crosslinks, and DNA single-strand breaks. The results of these experiments serve to form a basis for the quantitation of damages induced by exposure to sunlight.
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Calibrated immunoassays, electrophoretic analysis and chromatographic measurements give absolute lesion yields of 0.2 to 0.5 per 10^6 bases per J m^-2 upon UVB exposure of cultured cells.
“In terms of absolute yields of lesions, calibrated immunoassays, electrophoretic analysis and chromatographic measurements lead to values ranging between 0.2 and 0.5 per 10 6 bases per J m )2 upon exposure of cultured cells to UVB (55,61,62).”
From Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup> (2012) · cites this paper as [62]
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Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup>
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- Authors
- Rosenstein, B. S., & Mitchell, D. L.
- Affiliations
- Brown University (US)
- ORCID
- Mitchell, David L.
- Venue
- Radiation Research
- Publisher
- JSTOR
- Published
- 1991-06
- Volume
- 126
- Issue
- 3
- Pages
- 338
- ISSN
- 0033-7587 (ISSN-L)
- OpenAlex
- W2074224914
- Cited by
- 38
- References
- 31
- Citations by year
- 2016: 3 · 2015: 2 · 2014: 1 · 2012: 1
- FWCI
- 0.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Radiation Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01es004355-03, 5r01ca045078-08)
- National Cancer Institute (CA 45078)
- National Institute of Environmental Health Sciences (ES04355)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Keywords
- DNA repair, dermal fibroblasts, simulated sunlight, solar simulator, pyrimidine dimers, 6-4 photoproducts, Dewar isomers, DNA-protein crosslinks, single-strand breaks, ultraviolet radiation, sunburn
- MeSH terms
- Adult, Cells, Cultured, DNA Damage, DNA Repair, DNA-Binding Proteins, DNA, Single-Stranded, Endodeoxyribonucleases, Female, Fibroblasts, Humans, Pyrimidine Dimers, Skin, Ultraviolet Rays, Deoxyribonuclease (Pyrimidine Dimer)
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