CoChat
Sign Up
Cited by 156DNA Repair MechanismsCarcinogens and Genotoxicity AssessmentSkin Protection and Aging

DNA lesions induced by UV A1 and B radiation in human cells: Comparative analyses in the overall genome and in the p53 tumor suppressor gene

Open access
Besaratinia, Ahmad, Synold, Timothy W., Chen, Hsiu-Hua, Chang, Cheng, Xi, Bixin, Riggs, Arthur D., Pfeifer, Gerd P.·2005·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.0502311102PMC PMC1174921
Open this paper in CoChat — free account

Abstract

The UV components of sunlight (UVA and UVB) are implicated in the etiology of human skin cancer. The underlying mechanism of action for UVB carcinogenicity is well defined; however, the mechanistic involvement of UVA in carcinogenesis is not fully delineated. We investigated the genotoxicity of UVA1 versus UVB in the overall genome and in the p53 tumor suppressor gene in normal human skin fibroblasts. Immuno-dot blot analysis identified the cis - syn cyclobutane pyrimidine-dimer (CPD) as a distinctive UVB-induced lesion and confirmed its formation in the genomic DNA of UVA1-irradiated cells dependent on radiation dose. HPLC/tandem MS analysis showed an induction of 8-oxo-7,8-dihydro-2′-deoxyguanosine in the genomic DNA of UVA1-irradiated cells only. Mapping of DNA damages by terminal transferase-dependent PCR revealed preferential, but not identical, formation of polymerase-blocking lesions and/or strand breaks along exons 5-8 of the p53 gene in UVB- and UVA1-irradiated cells. The UVB-induced lesions detected by terminal transferase-PCR were almost exclusively mapped to pyrimidine-rich sequences; however, the UVA1-induced lesions were mapped to purine- and pyrimidine-containing sequences along the p53 gene. Cleavage assays with lesion-specific DNA repair enzymes coupled to ligation-mediated PCR showed preferential, but not identical, formation of CPDs along the p53 gene in UVB- and UVA1-irradiated cells. Additionally, dose-dependent formation of oxidized and ring-opened purines and abasic sites was established in the p53 gene in only UVA1-irradiated cells. We conclude that UVA1 induces promutagenic CPDs and oxidative DNA damage at both the genomic and nucleotide resolution level in normal human skin fibroblasts.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1073/pnas.0502311102

Cites

64 of 64 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • UVA radiation has been shown since the 1970s to induce CPDs in bacteria, mammalian cells and human skin.

    “In fact, UVA radiation has been shown since the 1970s, first in bacteria (29) and then in mammalian cells (32,55,(80)(81)(82)(83)(84) and human skin (61,65,(85)(86)(87), to be also able to induce CPDs.”

    From Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup> (2012) · cites this paper as [80]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 61

  1. [1]Woodhead, A. D., Setlow, R. B. & Tanaka, M. (1999) J. Epidemiol. 9, S102-S114. [DOI] [PubMed] [Google Scholar]
  2. [2]de Gruijl, F. R. (1999) Eur. J. Cancer 35, 2003-2009. [DOI] [PubMed] [Google Scholar]
  3. [3]Jhappan, C., Noonan, F. P. & Merlino, G. (2003) Oncogene 22, 3099-3112. [DOI] [PubMed] [Google Scholar]
  4. [4]Pfeifer, G. P., You, Y. H. & Besaratinia, A. (2005) Mutat. Res. 571, 19-31. [DOI] [PubMed] [Google Scholar]
  5. [5]Cadet, J., Sage, E. & Douki, T. (2005) Mutat. Res. 571, 3-17. [DOI] [PubMed] [Google Scholar]
  6. [6]Setlow, R. B. (1974) Proc. Natl. Acad. Sci. USA 71, 3363-3366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. [7]de Gruijl, F. R. (2002) Skin Pharmacol. Appl. Skin Physiol. 15, 316-320. [DOI] [PubMed] [Google Scholar]
  8. [8]de Gruijl, F. R., Sterenborg, H. J., Forbes, P. D., Davies, R. E., Cole, C., Kelfkens, G., van Weelden, H., Slaper, H. & van der Leun, J. C. (1993) Cancer Res. 53, 53-60. [PubMed] [Google Scholar]
  9. [9]Besaratinia, A., Synold, T. W., Xi, B. & Pfeifer, G. P. (2004) Biochemistry 43, 8169-8177. [DOI] [PubMed] [Google Scholar]
  10. [10]Besaratinia, A., Bates, S. E., Synold, T. & Pfeifer, G. P. (2004) Biochemistry 43, 15557-15566. [DOI] [PubMed] [Google Scholar]
  11. [11]Sutherland, J. C. & Griffin, K. P. (1981) Radiat. Res. 86, 399-409. [PubMed] [Google Scholar]
  12. [12]Kuluncsics, Z., Perdiz, D., Brulay, E., Muel, B. & Sage, E. %(1999%) J. Photochem. Photobiol. B 49, 71-80. [DOI] [PubMed] [Google Scholar]
  13. [13]Douki, T., Reynaud-Angelin, A., Cadet, J. & Sage, E. (2003) Biochemistry 42, 9221-9226. [DOI] [PubMed] [Google Scholar]
  14. [14]Rochette, P. J., Therrien, J. P., Drouin, R., Perdiz, D., Bastien, N., Drobetsky, E. A. & Sage, E. (2003) Nucleic Acids Res. 31, 2786-2794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. [15]Perdiz, D., Grof, P., Mezzina, M., Nikaido, O., Moustacchi, E. & Sage, E. (2000) J. Biol. Chem. 275, 26732-26742. [DOI] [PubMed] [Google Scholar]
  16. [16]Gray, B. A. & Gollin, S. M. (1987) Am. J. Med. Genet. 28, 521-526. [DOI] [PubMed] [Google Scholar]
  17. [17]Pfeifer, G. P., Chen, H. H., Komura, J. & Riggs, A. D. (1999) Methods Enzymol. 304, 548-571. [DOI] [PubMed] [Google Scholar]
  18. [18]Singh, R., McEwan, M., Lamb, J. H., Santella, R. M. & Farmer, P. B. (2003) Rapid Commun. Mass Spectrom. 17, 126-134. [DOI] [PubMed] [Google Scholar]
  19. [19]Mori, T., Nakane, M., Hattori, T., Matsunaga, T., Ihara, M. & Nikaido, O. (1991) Photochem. Photobiol. 54, 225-232. [DOI] [PubMed] [Google Scholar]
  20. [20]Chadwick, C. A., Potten, C. S., Nikaido, O., Matsunaga, T., Proby, C. & Young, A. R. (1995) J. Photochem. Photobiol. B 28, 163-170. [DOI] [PubMed] [Google Scholar]
  21. [21]You, Y. H., Lee, D. H., Yoon, J. H., Nakajima, S., Yasui, A. & Pfeifer, G. P. (2001) J. Biol. Chem. 276, 44688-44694. [DOI] [PubMed] [Google Scholar]
  22. [22]Yoon, J. H., Lee, C. S., O'Connor, T. R., Yasui, A. & Pfeifer, G. P. (2000) J. Mol. Biol. 299, 681-693. [DOI] [PubMed] [Google Scholar]
  23. [23]Freyer, G. A., Davey, S., Ferrer, J. V., Martin, A. M., Beach, D. & Doetsch, P. W. (1995) Mol. Cell. Biol. 15, 4572-4577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. [24]Yajima, H., Takao, M., Yasuhira, S., Zhao, J. H., Ishii, C., Inoue, H. & Yasui, A. (1995) EMBO J. 14, 2393-2399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. [25]Kaur, B., Avery, A. M. & Doetsch, P. W. (1998) Biochemistry 37, 11599-11604. [DOI] [PubMed] [Google Scholar]
  26. [26]Komura, J. & Riggs, A. D. (1998) Nucleic Acids Res. 26, 1807-1811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. [27]Chen, H. H., Kontaraki, J., Bonifer, C. & Riggs, A. D. (2001) Sci. STKE, PL1 (1-17). [DOI] [PubMed]
  28. [28]Pfeifer, G. P., Steigerwald, S. D., Mueller, P. R., Wold, B. & Riggs, A. D. (1989) Science 246, 810-813. [DOI] [PubMed] [Google Scholar]
  29. [29]Parrish, J. A., Jaenicke, K. F. & Anderson, R. R. (1982) Photochem. Photobiol. 36, 187-191. [DOI] [PubMed] [Google Scholar]
  30. [30]Robert, C., Muel, B., Benoit, A., Dubertret, L., Sarasin, A. & Stary, A. (1996) J. Invest. Dermatol. 106, 721-728. [DOI] [PubMed] [Google Scholar]
  31. [31]Daya-Grosjean, L. & Sarasin, A. (2005) Mutat. Res. 571, 43-56. [DOI] [PubMed] [Google Scholar]
  32. [32]Melnikova, V. O. & Ananthaswamy, H. N. (2005) Mutat. Res. 571, 91-106. [DOI] [PubMed] [Google Scholar]
  33. [33]Kosmoski, J. V. & Smerdon, M. J. (1999) Biochemistry 38, 9485-9494. [DOI] [PubMed] [Google Scholar]
  34. [34]Rosenstein, B. S. & Mitchell, D. L. (1987) Photochem. Photobiol. 45, 775-780. [DOI] [PubMed] [Google Scholar]
  35. [35]Taylor, J. S., Lu, H. F. & Kotyk, J. J. (1990) Photochem. Photobiol. 51, 161-167. [DOI] [PubMed] [Google Scholar]
  36. [36]Cadet, J., Douki, T., Pouget, J. P. & Ravanat, J. L. (2000) Methods Enzymol. 319, 143-153. [DOI] [PubMed] [Google Scholar]
  37. [37]Ravanat, J. L., Di Mascio, P., Martinez, G. R., Medeiros, M. H. & Cadet, J. (2000) J. Biol. Chem. 275, 40601-40604. [DOI] [PubMed] [Google Scholar]
  38. [38]Stary, A. & Sarasin, A. (2000) Methods Enzymol. 319, 153-165. [DOI] [PubMed] [Google Scholar]
  39. [39]Ravanat, J. L. & Cadet, J. (1995) Chem. Res. Toxicol. 8, 379-388. [DOI] [PubMed] [Google Scholar]
  40. [40]Randerath, K., Zhou, G. D., Somers, R. L., Robbins, J. H. & Brooks, P. J. (2001) J. Biol. Chem. 276, 36051-36057. [DOI] [PubMed] [Google Scholar]
  41. [41]Niles, J. C., Wishnok, J. S. & Tannenbaum, S. R. (2001) Org. Lett. 3, 963-966. [PubMed] [Google Scholar]
  42. [42]Bellon, S., Ravanat, J. L., Gasparutto, D. & Cadet, J. (2002) Chem. Res. Toxicol. 15, 598-606. [DOI] [PubMed] [Google Scholar]
  43. [43]Gu, C. & Wang, Y. (2004) Biochemistry 43, 6745-6750. [DOI] [PubMed] [Google Scholar]
  44. [44]Zhang, Q. & Wang, Y. (2005) Nucleic Acids Res. 33, 1593-1603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. [45]Kielbassa, C., Roza, L. & Epe, B. (1997) Carcinogenesis 18, 811-816. [DOI] [PubMed] [Google Scholar]
  46. [46]Zhang, X., Rosenstein, B. S., Wang, Y., Lebwohl, M., Mitchell, D. M. & Wei, H. (1997) Photochem. Photobiol. 65, 119-124. [DOI] [PubMed] [Google Scholar]
  47. [47]Cadet, J., Bellon, S., Berger, M., Bourdat, A. G., Douki, T., Duarte, V., Frelon, S., Gasparutto, D., Muller, E., Ravanat, J. L. & Sauvaigo, S. (2002) Biol. Chem. 383, 933-943. [DOI] [PubMed] [Google Scholar]
  48. [48]Lamola, A. A. (1968) Photochem. Photobiol. 8, 601-616. [DOI] [PubMed] [Google Scholar]
  49. [49]Sauvaigo, S., Douki, T., Odin, F., Caillat, S., Ravanat, J. L. & Cadet, J. (2001) Photochem. Photobiol. 73, 230-237. [DOI] [PubMed] [Google Scholar]
  50. [50]Agar, N. S., Halliday, G. M., Barnetson, R. S., Ananthaswamy, H. N., Wheeler, M. & Jones, A. M. (2004) Proc. Natl. Acad. Sci. USA 101, 4954-4959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  51. [51]Epe, B. (1991) Chem. Biol. Interact. 80, 239-260. [DOI] [PubMed] [Google Scholar]
  52. [52]Epe, B., Pflaum, M. & Boiteux, S. (1993) Mutat. Res. 299, 135-145. [DOI] [PubMed] [Google Scholar]
  53. [53]Darr, D. & Fridovich, I. (1994) J. Invest. Dermatol. 102, 671-675. [DOI] [PubMed] [Google Scholar]
  54. [54]Svoboda, D. L., Smith, C. A., Taylor, J. S. & Sancar, A. (1993) J. Biol. Chem. 268, 10694-10700. [PubMed] [Google Scholar]
  55. [55]Fritz, L. K. & Smerdon, M. J. (1995) Biochemistry 34, 13117-13124. [DOI] [PubMed] [Google Scholar]
  56. [56]Cleaver, J. E., Cortes, F., Karentz, D., Lutze, L. H., Morgan, W. F., Player, A. N., Vuksanovic, L. & Mitchell, D. L. (1988) Photochem. Photobiol. 48, 41-49. [DOI] [PubMed] [Google Scholar]
  57. [57]Cleaver, J. E., Jen, J., Charles, W. C. & Mitchell, D. L. (1991) Photochem. Photobiol. 54, 393-402. [DOI] [PubMed] [Google Scholar]
  58. [58]Nakajima, S., Lan, L., Kanno, S., Takao, M., Yamamoto, K., Eker, A. P. & Yasui, A. (2004) J. Biol. Chem. 279, 46674-46677. [DOI] [PubMed] [Google Scholar]
  59. [59]Dizdaroglu, M. (2003) Mutat. Res. 531, 109-126. [DOI] [PubMed] [Google Scholar]
  60. [60]Rosenstein, B. S. & Mitchell, D. L. (1991) Radiat. Res. 126, 338-342. [PubMed] [Google Scholar]
  61. [61]Maxam, A. M. & Gilbert, W. (1980) Methods Enzymol. 65, 499-560. [DOI] [PubMed] [Google Scholar]

Cited by

Sorted by citations

Related

Authors
Besaratinia, A., Synold, T. W., Chen, H.-H., Chang, C., Xi, B., Riggs, A. D., & Pfeifer, G. P.
Affiliations
  • Division of Biology, Beckman Research Institute, and Department of Medical Oncology, The City of Hope National Medical Center, 1450 East Duarte Road, Duarte, CA 91010; and Biotechnology Center, National Chung Hsing University, Taichung 40227, Taiwan
  • City Of Hope National Medical Center (US)
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2005-07-11
Volume
102
Issue
29
Pages
10058-10063
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.0502311102
PMC
PMC1174921
OpenAlex
W2150956753
Cited by
156
References
64
Citations by year
2026: 2 · 2025: 4 · 2024: 5 · 2023: 4 · 2022: 4 · 2021: 9 · 2020: 3 · 2019: 5 · 2018: 3 · 2017: 8 · 2016: 15 · 2015: 3 · 2014: 7 · 2013: 11 · 2012: 8
FWCI
4.66
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of Environmental Health Sciences (R37ES006070, R01ES006070, ES-06070)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
Keywords
UV-B radiation, DNA damage, p53 gene, cyclobutane pyrimidine dimers, 8-oxo-7,8-dihydro-2'-deoxyguanosine, oxidative DNA damage, abasic sites, dermal fibroblasts, skin cancer, DNA repair enzymes, strand breaks, genotoxicity
MeSH terms
Cells, Cultured, Chromatography, High Pressure Liquid, DNA Damage, Dose-Response Relationship, Radiation, Humans, Pyrimidine Dimers, Mass Spectrometry, Ultraviolet Rays, Immunoblotting, Genome, Human, Polymerase Chain Reaction, Genes, p53, DNA Repair Enzymes

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review