Inhibitory effect of Lactococcus lactis on the bioactivity of periodontopathogens
Open accessAbstract
Lactococcus lactis is a probiotic bacterium that produces various bacteriocins. Periodontopathogens induce inflammation and halitosis through the actions of lipopolysaccharide (LPS) and trypsin-like enzymes. The purpose of this study was to investigate the inhibitory effects of L. lactis on the bioactivity of periodontopathogens. To investigate the antimicrobial peptide of L. lactis, the spent culture medium (SCM) of L. lactis was treated with or without proteinase K after collection by centrifugation, and the antibacterial activity of SCM against periodontopathogens was assessed. To evaluate the neutralizing effect of L. lactis on halitosis, SCM of periodontopathogens was mixed with an L. lactis suspension, and the levels of volatile sulfur compounds (VSCs) were measured by gas chromatography. LPS from the periodontopathogens was extracted by an LPS extraction kit with little modification, and THP-1 cells as a monocytic cell line were treated with the extracted LPS in the presence or absence of UV-killed L. lactis. The production of inflammatory cytokines was analyzed by ELISA. The SCM of L. lactis exhibited antimicrobial activity against the periodontopathogens, whereas the proteinase K-treated SCM showed little antimicrobial activity. In addition, the L. lactis suspension had a neutralizing effect on the VSCs produced by periodontopathogens, and UV-killed L. lactis inhibited the production of IL-6 and TNF-α induced by the LPS. These results suggest that L. lactis may be a useful probiotic to prevent and treat periodontitis and halitosis.
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Postbiotics of Lactobacillus fermentum, Lactobacillus salivarius, and Lactococcus lactis can suppress the growth of opportunistic or pathogenic bacteria such as Streptococcus sanguinis, Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, Tannerella forsythia, and Treponema denticola.
“The postbiotics of Lactobacillus fermentum, Lactobacillus salivarius, and Lactococcus lactis can suppress the growth of opportunistic or pathogenic bacteria such as Streptococcus sanguinis, Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, Tannerella forsythia, and Treponema denticola [11,12].”
From Analysis of Chemical Structure and Antibiofilm Properties of Exopolysaccharides from Lactiplantibacillus plantarum EIR/IF-1 Postbiotics (2022) · cites this paper as [12]
The Discussion reports that spent culture medium from Lactococcus lactis inhibited growth of F. nucleatum, P. gingivalis, and T. forsythia; T. denticola was more resistant. The paper does not establish the claimed effects for Lactobacillus fermentum, Lactobacillus salivarius, Streptococcus sanguinis, or Streptococcus mutans. Page numbers were not available in our copy. (LLM, conf=0.88)
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“The postbiotics of Lactobacillus fermentum, Lactobacillus salivarius, and Lactococcus lactis can suppress the growth of opportunistic or pathogenic bacteria such as Streptococcus sanguinis, Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, Tannerella forsythia, and Treponema denticola [11,12].”
Background
- Authors
- Shin, H.-S., Baek, D.-H., & Lee, S.-H.
- Affiliations
- Dankook University (KR)
- Venue
- The Journal of General and Applied Microbiology
- Publisher
- Microbiology Research Foundation
- Published
- 2018
- Volume
- 64
- Issue
- 2
- Pages
- 55-61
- ISSN
- 0022-1260 (ISSN-L)
- 1349-8037
- OpenAlex
- W2791070863
- Cited by
- 23
- References
- 37
- Citations by year
- 2025: 4 · 2024: 1 · 2023: 4 · 2022: 6 · 2021: 3 · 2020: 1 · 2019: 4
- FWCI
- 2.29
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- The Journal of General and Applied Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Research Foundation
- National Research Foundation of Korea
- Ministry of Education, Science and Technology (NRF-2016R1C1B2007335)
- Topics
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Keywords
- Lactococcus lactis, periodontal pathogens, probiotics, bacteriocins, antimicrobial peptides, spent culture medium, volatile sulfur compounds, halitosis, lipopolysaccharide, inflammatory cytokines, interleukin-6, TNF-α, periodontitis, trypsin-like enzymes
- MeSH terms
- THP-1 Cells, Antibiosis, Bacteria, Humans, Lipopolysaccharides, Periodontitis, Lactococcus lactis, Sulfur Compounds, Cytokines, Culture Media, Conditioned, Bacterial Physiological Phenomena, Probiotics, Volatile Organic Compounds
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