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Lipopolysaccharides-Mediated Increase in Glucose-Stimulated Insulin Secretion: Involvement of the GLP-1 Pathway
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Lipopolysaccharides (LPS) of the cell wall of gram-negative bacteria trigger inflammation, which is associated with marked changes in glucose metabolism. Hyperglycemia is frequently observed during bacterial infection and it is a marker of a poor clinical outcome in critically ill patients. The aim of the current study was to investigate the effect of an acute injection or continuous infusion of LPS on experimentally induced hyperglycemia in wild-type and genetically engineered mice. The acute injection of a single dose of LPS produced an increase in glucose disposal and glucose-stimulated insulin secretion (GSIS). Continuous infusion of LPS through mini-osmotic pumps was also associated with increased GSIS. Finally, manipulation of LPS detoxification by knocking out the plasma phospholipid transfer protein (PLTP) led to increased glucose disposal and GSIS. Overall, glucose tolerance and GSIS tests supported the hypothesis that mice treated with LPS develop glucose-induced hyperinsulinemia. The effects of LPS on glucose metabolism were significantly altered as a result of either the accumulation or antagonism of glucagon-like peptide 1 (GLP-1). Complementary studies in wild-type and GLP-1 receptor knockout mice further implicated the GLP-1 receptor-dependent pathway in mediating the LPS-mediated changes in glucose metabolism. Hence, enhanced GLP-1 secretion and action underlies the development of glucose-mediated hyperinsulinemia associated with endotoxemia.
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“bacterial LPS were recently shown to enhance GLP-1 secretion, suggesting the involvement of TLR's in L cell activity (Larraufie et al., 2017;Nguyen et al., 2014).”
From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Nguyen et al., 2014
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Metadata
- Authors
- Nguyen, A. T., Mandard, S., Dray, C., Deckert, V., Valet, P., Besnard, P., Drucker, D. J., Lagrost, L., & Grober, J.
- Affiliations
- Inserm (FR)
- Université Toulouse III - Paul Sabatier (FR)
- Mount Sinai Hospital (CA)
- ORCID
- Venue
- Diabetes
- Publisher
- American Diabetes Association
- Published
- 2014-01-16
- Volume
- 63
- Issue
- 2
- Pages
- 471-482
- ISSN
- 0012-1797 (ISSN-L)
- 1939-327X
- OpenAlex
- W2129911161
- Cited by
- 161
- References
- 65
- Citations by year
- 2026: 11 · 2025: 16 · 2024: 12 · 2023: 13 · 2022: 19 · 2021: 12 · 2020: 13 · 2019: 19 · 2018: 9 · 2017: 11 · 2016: 12 · 2015: 8 · 2014: 6
- FWCI
- 8.53
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Diabetes (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- Funders
- Agence Nationale de la Recherche (ANR-11-LABX-0021)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pancreatic function and diabetes
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Treatment and Management
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Keywords
- lipopolysaccharide, endotoxemia, glucose-stimulated insulin secretion, glucagon-like peptide-1, GLP-1 receptor, hyperinsulinemia, glucose metabolism, hyperglycemia, phospholipid transfer protein, glucose tolerance, bacterial infection
- MeSH terms
- Glucagon-Like Peptide-1 Receptor, Animals, Blood Glucose, Glucose, Insulin, Lipopolysaccharides, Receptors, Glucagon, Mice, Knockout, Phospholipid Transfer Proteins, Mice, Glucagon-Like Peptide 1
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