Administration of CD4+CD25highCD127-regulatory T cells preserves β-cell function in type 1 diabetes in children
Open accessAbstract
OBJECTIVE: Type 1 diabetes is a condition in which pancreatic islets are destroyed by self-reactive T cells. The process is facilitated by deficits in the number and suppressive activity of regulatory T cells (Tregs). Here, we show for the first time that the infusion of autologous Tregs prolongs remission in recently diagnosed type 1 diabetes in children. RESEARCH DESIGN AND METHODS: We have administered Tregs in 10 type 1 diabetic children (aged 8–16 years) within 2 months since diagnosis. In total, 4 patients received 10 × 106 Tregs/kg body wt, and the remaining 6 patients received 20 × 106 Tregs/kg body wt. The preparation consisted of sorted autologous CD3+CD4+CD25highCD127− Tregs expanded under good manufacturing practice conditions. RESULTS: No toxicity of the therapy was noted. A significant increase in the percentage of Tregs in the peripheral blood has been observed since the day of infusion. These patients were followed along with matched type 1 diabetic patients not treated with Tregs. Half a year after type 1 diabetes onset (4–5 months after Tregs infusion), 8 patients treated with Tregs still required <0.5 UI/kg body wt of insulin daily, with 2 patients out of insulin completely, whereas the remission was over in the nontreated group. In addition, plasma C-peptide levels were significantly higher in the treated group as compared with those not treated. CONCLUSIONS: This study shows that the administration of Tregs is safe and tolerable in children with recent-onset type 1 diabetes.
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Autologous haemopoietic stem-cell transplantation and autologous T-regulatory cell administration are other novel approaches.
“Other novel approaches include autologous haemopoietic stem-cell transplantation165,166 and autologous T-regulatory cell administration.167–169”
From Type 1 diabetes (2018) · cites this paper as [169]
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- Authors
- Marek-Trzonkowska, N., Myśliwiec, M., Dobyszuk, A., Grabowska, M., Techmańska, I., Juścińska, J., Wujtewicz, M. A., Witkowski, P., Młynarski, W., Balcerska, A., Myśliwska, J., & Trzonkowski, P.
- Affiliations
- Gdańsk Medical University (PL)
- Blood Bank of the Pomerania Region, Gdańsk Unit, Gdańsk, Poland
- University of Chicago (US)
- Department of Pediatrics, Oncology, Hematology, and Diabetology, Medical University of Lodz, Lodz, Poland
- Corresponding author
- Trzonkowski, Piotr
- ORCID
- Venue
- Diabetes Care
- Publisher
- American Diabetes Association
- Published
- 2012-08-14
- Volume
- 35
- Issue
- 9
- Pages
- 1817-1820
- ISSN
- 0149-5992 (ISSN-L)
- 1935-5548
- PMC
- PMC3425004
- OpenAlex
- W2144147645
- Cited by
- 431
- References
- 22
- Citations by year
- 2026: 21 · 2025: 29 · 2024: 29 · 2023: 25 · 2022: 26 · 2021: 38 · 2020: 36 · 2019: 37 · 2018: 31 · 2017: 31 · 2016: 36 · 2015: 36 · 2014: 27 · 2013: 22 · 2012: 6
- FWCI
- 17.54
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- Diabetes Care (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Keywords
- type 1 diabetes, regulatory T cells, beta-cell function, remission, children, C-peptide, Treg expansion, immunotherapy safety
- MeSH terms
- Adolescent, Child, Diabetes Mellitus, Type 1, Female, Humans, Male, CD4-Positive T-Lymphocytes, T-Lymphocytes, Regulatory, Insulin-Secreting Cells, Interleukin-2 Receptor alpha Subunit, Interleukin-7 Receptor alpha Subunit
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