The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Evidence for Leptin Binding to Proteins in Serum of Rodents and Humans: Modulation With Obesity
Abstract
Many hormones circulate bound to serum proteins that modulate ligand bioactivity and bioavailability. To understand the biology of leptin action, we investigated the presence of leptin binding proteins in serum. 125I-labeled leptin binds competitively to at least three serum macromolecules with molecular masses of ∼ 85, ∼ 176, and ∼240 kDa in rodents and ∼176 and ∼240 kDa in humans. The ability to bind appears to involve sulfhydryl/disulfide interactions because it is inhibited under reducing conditions. When serum is added to recombinant 125I-leptin, there is a shift in sedimentation of 125I-leptin as analyzed by sucrose gradient centrifugation from ∼ S1.9 to ∼ S4.3. This shift is markedly attenuated in serum from obese mice (ob/ob, db/db, brown-fat ablated, gold-thioglucose treated, high-fat fed) compared with that from nonobese controls. The size distribution of endogenous serum leptin as determined by radioimmunoassay (RIA) after sucrose gradient centrifugation is also consistent with saturation of binding in hyperleptinemic obesity. In humans, free leptin increases with BMI. Thus, in lean rodents and humans a large proportion of leptin circulates bound to several serum proteins. Free leptin is increased in serum of obese subjects, which may alter leptin bioactivity, transport, and/or clearance.
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Cites
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Effects of the obese Gene Product on Body Weight Regulation in ob / ob Mice
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Abnormal splicing of the leptin receptor in diabetic mice
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Self-citationThe role of neuropeptide Y in the antiobesity action of the obese gene product
Stephens, T. W., Basinski, M., Bristow, P. K., Bue-Valleskey, J. M., Burgett, S. G., Craft, L., Hale, J., Hoffmann, J., Hsiung, H. M., Kriauciunas, A., et al. (1995) · Nature · doi:10.1038/377530a0 · Cited by 1,498
Development of obesity in transgenic mice after genetic ablation of brown adipose tissue
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Self-citationSchwartz, M. W., Baskin, D. G., Bukowski, T. R., Kuijper, J. L., Foster, D., Lasser, G., Prunkard, D. E., Porte, D., Woods, S. C., Seeley, R. J., et al. (1996) · Diabetes · doi:10.2337/diab.45.4.531 · Cited by 904 · cited 1×
“The expression of leptin and neuropeptide Y appear to be reciprocally regulated (6-8).”
Background
Cited by
Subcutaneous and visceral adipose tissue: their relation to the metabolic syndrome
Wajchenberg, B. L. (2000) · Endocrine Reviews · doi:10.1210/edrv.21.6.0415 · Cited by 2,961
Thyroid Hormone Regulation of Metabolism
Mullur, R., Liu, Y.-Y., & Brent, G. A. (2014) · Physiological Reviews · doi:10.1152/physrev.00030.2013 · Cited by 2,421
A mutation in the human leptin receptor gene causes obesity and pituitary dysfunction
Clément, K., Vaisse, C., Lahlou, N., Cabrol, S., Pelloux, V., Cassuto, D., Gourmelen, M., Dina, C., Chambaz, J., Lacorte, J.-M., et al. (1998) · Nature · doi:10.1038/32911 · Cited by 2,346
Ahima, R. S., & Flier, J. S. (2000) · Annual Review of Physiology · doi:10.1146/annurev.physiol.62.1.413 · Cited by 1,428
Self-citationLeptin Regulation of Neuroendocrine Systems
Ahima, R. S., Saper, C. B., Flier, J. S., & Elmquist, J. K. (2000) · Frontiers in Neuroendocrinology · doi:10.1006/frne.2000.0197 · Cited by 767
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Metadata
- Authors
- Houseknecht, K. L., Mantzoros, C. S., Kuliawat, R., Hadro, E., Flier, J. S., & Kahn, B. B.
- Venue
- Diabetes
- Publisher
- American Diabetes Association
- Published
- 1996-11-01
- Volume
- 45
- Issue
- 11
- Pages
- 1638-1643
- ISSN
- 0012-1797 (ISSN-L)
- 1939-327X
- OpenAlex
- W2140925859
- Cited by
- 245
- References
- 8
- FWCI
- 7.20
- Type
- journal article
- Language
- en
- Open access
- closed
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- Houseknecht, K. L., Mantzoros, C. S., Kuliawat, R., Hadro, E., Flier, J. S., & Kahn, B. B. (1996). Evidence for Leptin Binding to Proteins in Serum of Rodents and Humans: Modulation With Obesity. Diabetes. https://doi.org/10.2337/diab.45.11.1638
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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