The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Exercise Increases Nuclear AMPK α2 in Human Skeletal Muscle
Open accessAbstract
An acute bout of exercise increases skeletal muscle glucose uptake, improves glucose homeostasis and insulin sensitivity, and enhances muscle oxidative capacity. Recent studies have shown an association between these adaptations and the energy-sensing 5' AMP-activated protein kinase (AMPK), the activity of which is increased in response to exercise. Activation of AMPK has been associated with enhanced expression of key metabolic proteins such as GLUT-4, hexokinase II (HKII), and mitochondrial enzymes, similar to exercise. It has been hypothesized that AMPK might regulate gene and protein expression through direct interaction with the nucleus. The purpose of this study was to determine if nuclear AMPK alpha(2) content in human skeletal muscle was increased by exercise. Following 60 min of cycling at 72 +/- 1% of VO(2peak) in six male volunteers (20.6 +/- 2.1 years; 72.9 +/- 2.1 kg; VO(2peak) = 3.62 +/- 0.18 l/min), nuclear AMPK alpha(2) content was increased 1.9 +/- 0.4-fold (P = 0.024). There was no change in whole-cell AMPK alpha(2) content or AMPK alpha(2) mRNA abundance. These results suggest that nuclear translocation of AMPK might mediate the effects of exercise on skeletal muscle gene and protein expression.
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“In a feed forward fashion AMPK can translocate to the nucleus [24,27] where it regulates Histone de-aceytlases, transcription factors and transcriptional co-activators such as histone deacetylase 5 (HDAC5), myocyte enhancing factor 2 (MEF2) and peroxisome proliferator activated receptor-γ co-activator-1α (PGC-1α) respectively (Figure 1) [28][29][30].”
From New strategies in sport nutrition to increase exercise performance (2016) · cites this paper as [27]
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Cites
Activation of AMP-activated protein kinase increases mitochondrial enzymes in skeletal muscle
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Chronic activation of AMP kinase results in NRF-1 activation and mitochondrial biogenesis
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Dealing with energy demand: the AMP-activated protein kinase
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Mechanism for Fatty Acid “Sparing” Effect on Glucose-induced Transcription
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“AMPK complexes containing the catalytic 2 subunit are preferentially activated during exercise at intensities 70% VO2peak, in both normal (3) and type 2 diabetic subjects (4).”
BackgroundLo, W., Duggan, L. J., Tolga, N. C., Emre, N. C. T., Belotserkovskya, R., Lane, W. S., Shiekhattar, R., & Berger, S. L. (2001) · Science · doi:10.1126/science.1062322 · Cited by 369
Cited by
Sorted by citations
AMPK: guardian of metabolism and mitochondrial homeostasis
Herzig, S., & Shaw, R. J. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.95 · Cited by 4,192
Steinberg, G. R., & Kemp, B. E. (2009) · Physiological Reviews · doi:10.1152/physrev.00011.2008 · Cited by 1,672
Self-citationExercise, GLUT4, and skeletal muscle glucose uptake
Richter, E. A., & Hargreaves, M. (2013) · Physiological Reviews · doi:10.1152/physrev.00038.2012 · Cited by 1,407
Self-citationAMPK and PPARδ Agonists Are Exercise Mimetics
Narkar, V. A., Downes, M., Yu, R. T., Embler, E., Wang, Y.-X., Banayo, E., Mihaylova, M. M., Nelson, M. C., Zou, Y., Juguilon, H., et al. (2008) · Cell · doi:10.1016/j.cell.2008.06.051 · Cited by 1,222
AMPK and SIRT1: a long-standing partnership?
Ruderman, N. B., Julia Xu, X., Nelson, L., Cacicedo, J. M., Saha, A. K., Lan, F., & Ido, Y. (2010) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00745.2009 · Cited by 903
Metadata
- Authors
- McGee, S. L., Howlett, K. F., Starkie, R. L., Cameron-Smith, D., Kemp, B. E., & Hargreaves, M.
- Affiliations
- Deakin University (AU)
- Exercise, Muscle and Metabolism Unit, School of Health Sciences, Deakin University, Burwood, Australia
- St Vincent's Hospital Melbourne (AU)
- Venue
- Diabetes
- Publisher
- American Diabetes Association
- Published
- 2003-04-01
- Volume
- 52
- Issue
- 4
- Pages
- 926-928
- ISSN
- 0012-1797 (ISSN-L)
- 1939-327X
- OpenAlex
- W2124798116
- Cited by
- 160
- References
- 25
- Citations by year
- 2026: 6 · 2025: 3 · 2024: 6 · 2023: 3 · 2022: 4 · 2021: 6 · 2020: 12 · 2019: 2 · 2018: 3 · 2017: 7 · 2016: 10 · 2015: 10 · 2014: 7 · 2013: 8 · 2012: 9
- FWCI
- 2.77
- Type
- journal article
- Language
- en
- Open access
- green
- figshare.com
- Licence
- cc-by
- Locations
- Diabetes (publishedVersion)
- PubMed (publishedVersion)
- Figshare (submittedVersion, cc-by)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Health Sciences › Medicine › Physiology (medical) › Cardiovascular and exercise physiology
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Keywords
- AMPK, AMPKα2, nuclear translocation, physical activity, skeletal muscle, GLUT4, hexokinase 2, insulin sensitivity, glucose uptake, mitochondrial enzymes, gene expression regulation, cycling exercise
- MeSH terms
- Adult, Cell Nucleus, Humans, Male, Multienzyme Complexes, Oxygen Consumption, RNA, Messenger, Time Factors, Exercise, Protein Serine-Threonine Kinases, Muscle, Skeletal, AMP-Activated Protein Kinases
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