Cell-bound exopolysaccharide extract from indigenous probiotic bacteria induce apoptosis in HT–29 cell-line
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Background and Objective: The aim of this study was to compare the cytotoxic effects of local probiotic bacteria, including Lactobacillus paracasei, Lactobacillus brevis, while isolated from "Tarkhine" food and the induction of apoptosis in the HT-29 human colon adenocarcinoma cell line and normal fibroblasts.Methods: HT-29 and L-929 cell lines were treated with cell-bound exopolysaccharide extract (cb-EPS) from L. paracasei and L. brevis.The MTT assay was used to analyze cell viability.Cellular apoptosis was examined by flow cytometry and DNA fragmentation assay.Results: The cb-EPS from both probiotic bacteria prevented the proliferation of HT-29 colon cancer cells.In addition, the cytotoxic and anti-proliferative effects of the exopolysaccharide extract from both bacteria in L-929 fibroblasts were much lower than HT-29 cells.The induction of apoptosis in HT-29 cells was observed at 48h compared with 72h.It seems that the exopolysaccharides extracted from both bacteria have a greater effect on the induction of apoptosis at 48h.The cb-EPS of L. brevis showed more potent anti-proliferative and apoptotic properties than the cb-EPS of L. paracasei.The ladder pattern of DNA fragmentation confirmed the induction of apoptosis in cancer cells. Conclusion:The results of the MTT assay and apoptosis indicate that the induction of apoptosis by the exopolysaccharide from bacteria depends on the dose, time, and strain of bacteria.Further studies may contribute toward the understanding of using these probiotic bacteria as biological products to treat and prevent cancers. Lactobacillus,Probiotic,
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The apoptotic induction of colon cancer cell death as characterized by fragmented DNA by potential probiotics has also been studied using other strains, such as L. acidophilus 606, L. brevis and L. paracasei.
“The apoptotic induction of colon cancer cell death as characterized by fragmented DNA by the potential probiotics has also been studied using other strains, such as L. acidophilus 606 [59], L. brevis [60] and L. paracasei [61].”
From Bioprophylactic potential of novel human colostrum probiotics via apoptotic induction of colon cancer cells and cell immune activation (2022) · cites this paper as [60]
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- Mojibi P, Tafvizi F, Bikhof Torbati M. Cell-bound Exopolysaccharide Extract from Indigenous Probiotic Bacteria Induce Apoptosis in HT-29 cell-line. Iranian Journal of Pathology. 2019; 14(1): 41-51. How to Cite This Article
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Srikham, K., & Thirabunyanon, M. (2022) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2022.112871 · Cited by 24 · see citing works
- Authors
- Mojibi, P.
- Affiliations
- Islamic Azad University of Parand (IR)
- Corresponding author
- Mojibi, Parisa
- Venue
- Iranian journal of pathology
- Publisher
- Farname, Inc.
- Published
- 2018-12-01
- Volume
- 14
- Issue
- 1
- Pages
- 41-51
- ISSN
- 1735-5303 (ISSN-L)
- PMC
- PMC6708565
- OpenAlex
- W2913549218
- Cited by
- 40
- References
- 24
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 8 · 2023: 8 · 2022: 7 · 2021: 4 · 2020: 3 · 2019: 3 · 2018: 1
- FWCI
- 2.58
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Iranian journal of pathology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Islamic Azad University
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Cancer Research and Treatments
- Keywords
- probiotic bacteria, Lactobacillus paracasei, Lactobacillus brevis, apoptosis, HT-29 cells, antiproliferative activity, DNA fragmentation, L929 fibroblasts, cancer prevention
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