Exposure of single-walled carbon nanotubes impairs the functions of primarily cultured murine peritoneal macrophages
Abstract
It is increasingly important to understand the single-walled carbon nanotubes' (SWCNTs) immune response as their increasingly biomedical researches and applications. Macrophages and T cells play important roles in scavenging foreign materials and pathogens and regulating immune response. In this work, primarily cultured murine peritoneal macrophages and purified splenic T cells were utilised to determine the toxic effects of SWCNTs and acid-functionalised SWCNTs (AF-SWCNTs) on the immune system, especially on macrophage functions. Macrophages were exposed to 0-50 μg/ml of CNTs for 24 h and no significant cytotoxicity was found by live/dead and annexin-V-FITC/PI analyses. The TEM images revealed that AF-SWCNTs were engulfed mostly through phagocytosis and located in lysosomes of macrophages. Measurement of mitochondrial membrane potential and proteasome subunit gene expression demonstrated that 10 and 50 μg/ml AF-SWCNTs could damage mitochondrial function and proteasome formation in a concentration-dependent manner. Functional analyses revealed that the percentage of phagocytic cells were affected significantly by 20 μg/ml CNTs, and 5 μg/ml AF-SWCNTs inhibited the phagocytic efficiency of latex beads in macrophages. The accessory cell function was affected by both AF-SWCNTs and SWCNTs at concentrations of 10 and 50 μg/ml, respectively. Furthermore, AF-SWCNT biased naïve T-cell differentiation to Th1 type by inducing the production of IFN-γ and TNF, implying the potential risk of Th1-associated diseases (e.g. autoimmune diseases and inflammation) on AF-SWCNT exposure.
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Green, D. R., & Reed, J. C. (1998) · Science · doi:10.1126/science.281.5381.1309 · Cited by 8,998 · see citing works
Mosmann, T. R., & Coffman, R. L. (1989) · Annual Review of Immunology · doi:10.1146/annurev.iy.07.040189.001045 · Cited by 7,692 · see citing works
Pfaffl, M. W. (2002) · Nucleic Acids Research · doi:10.1093/nar/30.9.e36 · Cited by 7,377 · see citing works
Functional diversity of helper T lymphocytes
Abbas, A. K., MURPHY, K. M. M., & Sher, A. (1996) · Nature · doi:10.1038/383787a0 · Cited by 4,383 · see citing works
Current Protocols in Immunology
(2018) · Current Protocols in Immunology · doi:10.1002/(issn)1934-368x · Cited by 3,574 · see citing works
Liu, J., Rinzler, A. G., Dai, H., et al. (1998) · Science · doi:10.1126/science.280.5367.1253 · Cited by 2,904 · see citing works
Effect of Surface Properties on Nanoparticle–Cell Interactions
Verma, A., & Stellacci, F. (2009) · Small · doi:10.1002/smll.200901158 · Cited by 2,621 · see citing works
Ultrafine particulate pollutants induce oxidative stress and mitochondrial damage
Li, N., Sioutas, C., Cho, A., Schmitz, D., Misra, C., Sempf, J., Wang, M., Oberley, T., Froines, J., & Nel, A. (2003) · Environmental Health Perspectives · doi:10.1289/ehp.6000 · Cited by 2,097 · see citing works
Applications of Carbon Nanotubes in Drug Delivery
Kaur, J., Gill, G. S., & Jeet, K. (2005) · Elsevier eBooks · doi:10.1016/b978-0-12-814031-4.00005-2 · Cited by 2,023 · see citing works
Immunological properties of engineered nanomaterials
Dobrovolskaia, M. A., & McNeil, S. E. (2007) · Nature Nanotechnology · doi:10.1038/nnano.2007.223 · Cited by 1,823 · see citing works
Carbon Nanotubes in Biology and Medicine: In vitro and in vivo Detection, Imaging and Drug Delivery
Liu, Z., Tabakman, S., Welsher, K. D., & Dai, H. (2009) · Nano Reseach · doi:10.1007/s12274-009-9009-8 · Cited by 1,629 · see citing works
In vivo biodistribution and highly efficient tumour targeting of carbon nanotubes in mice
Liu, Z., Cai, W., He, L., Nakayama-Ratchford, N., Chen, K., Sun, X., Chen, X. S., & Dai, H. (2006) · Nature Nanotechnology · doi:10.1038/nnano.2006.170 · Cited by 1,440 · see citing works
Current protocols in immunology
Nezlin, R. S. (1992) · Molecular Immunology · doi:10.1016/0161-5890(92)90114-d · Cited by 1,418 · see citing works
Cytotoxicity of Carbon Nanomaterials: Single-Wall Nanotube, Multi-Wall Nanotube, and Fullerene
Jia, G., Wang, H., Yan, L., Wang, X., Pei, R., Yan, T., Zhao, Y., & Guo, X. (2005) · Environmental Science & Technology · doi:10.1021/es048729l · Cited by 1,404 · see citing works
Kam, N. W. S., Jessop, T. C., Wender, P. A., & Dai, H. (2004) · Journal of the American Chemical Society · doi:10.1021/ja0486059 · Cited by 1,351 · see citing works
Drug Delivery with Carbon Nanotubes for In vivo Cancer Treatment
Liu, Z., Chen, K., Davis, C. R., Sherlock, S. P., Cao, Q., Chen, X. S., & Dai, H. (2008) · Cancer Research · doi:10.1158/0008-5472.can-08-1468 · Cited by 1,349 · see citing works
In vivo Biodistribution and Highly Efficient Tumour Targeting of Carbon Nanotubes in Mice
Liu, Z., Cai, W., He, L., Nakayama-Ratchford, N., Chen, K., Sun, X., Chen, X. S., & Dai, H. (2020) · doi:10.1201/9780429399039-14 · Cited by 1,213 · see citing works
Functionalization density dependence of single-walled carbon nanotubes cytotoxicity in vitro
Sayes, C. M., Liang, F., Hudson, J. L., Mendez, J. S., Guo, W., Beach, J. M., Moore, V. C., Doyle, C. D., West, J. L., Billups, W. E., et al. (2005) · Toxicology Letters · doi:10.1016/j.toxlet.2005.08.011 · Cited by 856 · see citing works
Carbon nanotubes degraded by neutrophil myeloperoxidase induce less pulmonary inflammation
Kagan, V. E., Konduru, N. V., Feng, W., Allen, B. L., Conroy, J., Volkov, Y., Vlasova, I. I., Belikova, N. A., Yanamala, N. V. K., Kapralov, A. A., et al. (2010) · Nature Nanotechnology · doi:10.1038/nnano.2010.44 · Cited by 796 · see citing works
Dumortier, H., Lacotte, S., Pastorin, G., Marega, R., Wu, W., Bonifazi, D., Briand, J., Prato, M., Muller, S., & Bianco, A. (2006) · Nano Letters · doi:10.1021/nl061160x · Cited by 697 · see citing works
Manna, S. K., Sarkar, S., Barr, J., Wise, K., Barrera, E. V., Jejelowo, O., Rice-Ficht, A. C., & Ramesh, G. T. (2005) · Nano Letters · doi:10.1021/nl0507966 · Cited by 577 · see citing works
Direct imaging of single-walled carbon nanotubes in cells
Porter, A. E., Gass, M., Muller, K., Skepper, J. N., Midgley, P. A., & Welland, M. (2007) · Nature Nanotechnology · doi:10.1038/nnano.2007.347 · Cited by 564 · see citing works
True solutions of single-walled carbon nanotubes for assembly into macroscopic materials
Davis, V. A., Parra‐Vasquez, A. N. G., Green, M. J., et al. (2009) · Nature Nanotechnology · doi:10.1038/nnano.2009.302 · Cited by 561 · see citing works
Hong, J., Heller, D. A., Sharma, R., & Strano, M. S. (2009) · ACS Nano · doi:10.1021/nn800532m · Cited by 544 · see citing works
Wang, L., Liu, Y., Li, W., et al. (2010) · Nano Letters · doi:10.1021/nl103992v · Cited by 514 · see citing works
Biodegradation of Single-Walled Carbon Nanotubes through Enzymatic Catalysis
Allen, B. L., Kichambare, P., Gou, P., Vlasova, I. I., Kapralov, A. A., Konduru, N. V., Kagan, V. E., & Star, A. (2008) · Nano Letters · doi:10.1021/nl802315h · Cited by 435 · see citing works
Rapidly Functionalized, Water-Dispersed Carbon Nanotubes at High Concentration
Wang, Y., Iqbal, Z., & Mitra, S. (2005) · Journal of the American Chemical Society · doi:10.1021/ja053003q · Cited by 391 · see citing works
Making carbon nanotubes biocompatible and biodegradable
Bianco, A., Kostarelos, K., & Prato, M. (2011) · Chemical Communications · doi:10.1039/c1cc13011k · Cited by 385 · see citing works
Risk assessment of engineered nanomaterials and nanotechnologies—A review
Savolainen, K., Alenius, H. T., Norppa, H., Pylkkänen, L., Tuomi, T., & Kasper, G. (2010) · Toxicology · doi:10.1016/j.tox.2010.01.013 · Cited by 381 · see citing works
Mechanisms for how inhaled multiwalled carbon nanotubes suppress systemic immune function in mice
Mitchell, L., Lauer, F. T., Burchiel, S. W., et al. (2009) · Nature Nanotechnology · doi:10.1038/nnano.2009.151 · Cited by 326 · see citing works
Allen, B. L., Kotchey, G. P., Chen, Y., et al. (2009) · Journal of the American Chemical Society · doi:10.1021/ja9083623 · Cited by 315 · see citing works
Single-Walled Carbon Nanotubes Can Induce Pulmonary Injury in Mouse Model
Chou, C., Hsiao, H.-Y., Hong, Q., Chen, C., Peng, Y., Chen, H., & Yang, P. (2008) · Nano Letters · doi:10.1021/nl0723634 · Cited by 301 · see citing works
Fullerene Nanomaterials Inhibit the Allergic Response
Ryan, J. J., Bateman, H. R., Stover, A., Gomez, G., Norton, S. K., Zhao, W., Schwartz, L. B., Lenk, R., & Kepley, C. L. (2007) · The Journal of Immunology · doi:10.4049/jimmunol.179.1.665 · Cited by 273 · see citing works
Cell-penetrating CNTs for delivery of therapeutics
Lacerda, L., Raffa, V., Prato, M., et al. (2007) · Nano Today · doi:10.1016/s1748-0132(07)70172-x · Cited by 242 · see citing works
Mu, Q., Broughton, D. L., & Yan, B. (2009) · Nano Letters · doi:10.1021/nl902647x · Cited by 240 · see citing works
Liu, Y., Jiao, F., Qiu, Y., Li, W. X., Lao, F., Zhou, G., Sun, B., Xing, G., Dong, J., Zhao, Y., et al. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.04.001 · Cited by 197 · see citing works
Sequential Exposure to Carbon Nanotubes and Bacteria Enhances Pulmonary Inflammation and Infectivity
Shvedova, A. A., Fabisiak, J. P., Kisin, E. R., Murray, A. R., Roberts, J. R., Tyurina, Y. Y., Antonini, J. M., Feng, W. H., Kommineni, C., Reynolds, J. S., et al. (2007) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/rcmb.2007-0255oc · Cited by 172 · see citing works
Vives-Bauza, C., Anand, M., Shirazi, A. K., Magrane, J., Gao, J., Vollmer-Snarr, H. R., Manfredi, G., & Finnemann, S. C. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m800706200 · Cited by 155 · see citing works
Fournier, M., Cyr, D. G., Blakley, B. R., et al. (2000) · American Zoologist · doi:10.1093/icb/40.3.412 · Cited by 147 · see citing works
[Gd@C82(OH)22]n Nanoparticles Induce Dendritic Cell Maturation and Activate Th1 Immune Responses
Yang, D., Zhao, Y., Guo, H., Li, Y., Tewary, P., Xing, G., Hou, W., Oppenheim, J. J., & Zhang, N. (2010) · ACS Nano · doi:10.1021/nn901478z · Cited by 144 · see citing works
Proliferative Assays for T Cell Function
Kruisbeek, A. M., Shevach, E. M., & Thornton, A. M. (2004) · Current Protocols in Immunology · doi:10.1002/0471142735.im0312s60 · Cited by 142 · see citing works
The immunomodulatory effect(s) of lead and cadmium on the cells of immune system in vitro
Krocova, Z., Macela, A., Kroča, M., et al. (2000) · Toxicology in Vitro · doi:10.1016/s0887-2333(99)00089-2 · Cited by 134 · see citing works
Waters, K. M. (2001) · Toxicological Sciences · doi:10.1093/toxsci/63.1.47 · Cited by 129 · see citing works
Konduru, N. V., Tyurina, Y. Y., Feng, W., Basova, L. V., Belikova, N. A., Bayır, H., Clark, K., Rubin, M. A., Stolz, D. B., Vallhov, H., et al. (2009) · PLoS ONE · doi:10.1371/journal.pone.0004398 · Cited by 118 · see citing works
Flt3+ macrophage precursors commit sequentially to osteoclasts, dendritic cells and microglia
Delprat, C., Arnaud, S., Jurdic, P., Nataf, S., Grasset, M.-F., Soulas, C., Domenget, C., Destaing, O., Rivollier, A., Perret, M., et al. (2002) · BMC Immunology · doi:10.1186/1471-2172-3-15 · Cited by 101 · see citing works
Gao, N., Zhang, Q., Mu, Q., Bai, Y., Li, L., Zhou, H., Butch, E. R., Powell, T. B., Snyder, S. E., Jiang, G., et al. (2011) · ACS Nano · doi:10.1021/nn200283g · Cited by 89 · see citing works
Saxena, R. K., Williams, W. C., McGee, J. K., et al. (2007) · Nanotoxicology · doi:10.1080/17435390701803110 · Cited by 82 · see citing works
Wan, B., Fleming, J. T., Schultz, T. W., et al. (2005) · Environmental Health Perspectives · doi:10.1289/ehp.8085 · Cited by 71 · see citing works
Self-citationTeo, S. K., Sabourin, P. J., O'Brien, K., Kook, K. A., & Thomas, S. D. (2000) · Journal of Biochemical and Molecular Toxicology · doi:10.1002/(sici)1099-0461(2000)14:3 · Cited by 67 · see citing works
Lei, J., & Ju, H. (2010) · Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology · doi:10.1002/wnan.94 · Cited by 57 · see citing works
Single-walled carbon nanotubes impair human macrophage engulfment of apoptotic cell corpses
Witasp, E., Shvedova, A. A., Kagan, V. E., et al. (2009) · Inhalation Toxicology · doi:10.1080/08958370902942574 · Cited by 56 · see citing works
Role of Ubiquitin and Proteasomes in Phagosome Maturation
Lee, W. L., Kim, M.-K., Schreiber, A. D., & Grinstein, S. (2005) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-06-0464 · Cited by 53 · see citing works
Embleton, M. J. (2003) · British Journal of Cancer · doi:10.1038/sj.bjc.6601103 · Cited by 51 · see citing works
Pollard, K. M., & Landberg, G. (2001) · International Immunopharmacology · doi:10.1016/s1567-5769(00)00034-5 · Cited by 49 · see citing works
Dong, P., Wan, B., & Guo, L. (2011) · Nanotoxicology · doi:10.3109/17435390.2011.573101 · Cited by 37 · see citing works
Self-citationMulti-walled carbon nanotubes do not impair immune functions of dendritic cells
Wang, J., Sun, R. H., Zhang, N., Nie, H., Liu, J.-H., Junrong, W. A. N. G., Wang, H., & Liu, Y. (2009) · Carbon · doi:10.1016/j.carbon.2009.03.003 · Cited by 36 · see citing works
Effects of Lead on IQ in Children
Ernhart, C. B. (2006) · Environmental Health Perspectives · doi:10.1289/ehp.114-a85 · Cited by 30 · see citing works
Ye, S., Zhang, H., Wang, Y., Jiao, F., Lin, C., & Zhang, Q. (2011) · Journal of Nanoparticle Research · doi:10.1007/s11051-011-0368-1 · Cited by 15 · see citing works
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- Authors
- Dong, P.-X., Wan, B., Wang, Z.-X., Guo, L.-H., Yang, Y., & Zhao, L.
- Affiliations
- Chinese Academy of Sciences (CN)
- Corresponding author
- Wan, Bin
- Guo, Liang-Hong
- Venue
- Nanotoxicology
- Publisher
- Informa UK Limited
- Published
- 2012-06-14
- Volume
- 7
- Issue
- 5
- Pages
- 1028-1042
- ISSN
- 1743-5390 (ISSN-L)
- 1743-5404
- OpenAlex
- W2131678403
- Cited by
- 42
- References
- 58
- Citations by year
- 2026: 6 · 2025: 1 · 2024: 1 · 2023: 3 · 2022: 5 · 2021: 3 · 2020: 2 · 2019: 3 · 2017: 4 · 2016: 4 · 2015: 3 · 2014: 2 · 2013: 3 · 2012: 2
- FWCI
- 1.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nanotoxicology (publishedVersion)
- PubMed (publishedVersion)
- Research Center for Eco-Environmental Sciences OpenIR (Chinese Academy of Sciences) (submittedVersion)
- Funders
- National Science Foundation
- National Natural Science Foundation of China
- National Key Research and Development Program of China
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Graphene and Nanomaterials Applications
- Life Sciences › Immunology and Microbiology › Immunology › Immunotoxicology and immune responses
- Physical Sciences › Materials Science › Materials Chemistry › Carbon Nanotubes in Composites
- Keywords
- single-walled carbon nanotubes, peritoneal macrophages, macrophage phagocytosis, mitochondrial membrane potential, accessory cell function, T cell differentiation, Th1 polarization, interferon-gamma, TNF-α, nanoparticle toxicity, immune response, lysosomal uptake
- MeSH terms
- Animals, Cell Differentiation, Cell Survival, Cells, Cultured, Female, T-Lymphocytes, Helper-Inducer, Lysosomes, Phagocytosis, Gene Expression, Cytokines, Macrophages, Peritoneal, Nanotubes, Carbon, Intracellular Space, Microscopy, Electron, Transmission, Cell Proliferation, Mice
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