The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Influence of ensiling time and inoculation on alteration of the starch-protein matrix in high-moisture corn
Open accessAbstract
The fates of hydrophobic zein proteins, which encapsulate corn starch to create vitreous endosperm, have not been investigated in high-moisture corn (HMC). To assess influences of ensiling time and inoculation on zein proteins in HMC, quadruplicate samples of 2 random corn hybrids (A and B), containing 25.7 and 29.3% moisture, were ground, inoculated with (I) or without 600,000 cfu/g of Lactobacillus buchneri 40788 (Lallemand Animal Nutrition, Milwaukee, WI), and ensiled for 0, 15, 30, 60, 120, and 240 d. Nutrient composition [crude protein (CP), starch, acid detergent fiber, and neutral detergent fiber], fermentation (pH, lactate, and acetate), and protein degradation markers (buffer-soluble CP, isopropanol-soluble CP, and NH(3)-N) were evaluated. At 0 and 240 d, α, γ, δ, and β zein subunits were profiled using HPLC. Data were evaluated as a split-split plot using the PROC MIXED procedures of SAS. Ensiling time and inoculation decreased pH, and altered lactate and acetate contents of HMC. Lactate and acetate contents of A, AI, B, and BI at 240 d were 0.40, 0.32, 1.11, 0.73, and 0, 0.35, 0.30, and 0.87% of DM, respectively. Buffer-soluble CP in HMC increased from 1.5 to 2.0% of DM at 0 d to >4.0% of DM at 240 d. Inoculation had no effect on buffer-soluble CP but increased NH(3)-N content of HMC. Corn A contained more isopropanol-soluble CP than did corn B and peak areas for 6 α, and all γ and δ zein regions were greater for corn A. Ensiling (0 vs. 240 d) decreased all zein subunits with the exception of 2 α and 1 δ subunit. Ensiling decreased (42.2-73.2%) γ zeins, which are primarily responsible for cross-linking in the starch-protein matrix. Despite altering lactate and acetate contents, inoculation had no effect on degrading hydrophobic zein proteins in HMC. Data suggest that hydrophobic zein proteins in the starch-protein matrix of HMC are degraded by proteolytic activity over an extended ensiling time.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Proteolysis during silo storage slowly breaks the protein matrix so that starch digestibility increases with storage time (Hoffman et al., 2011), as discussed above.”
From Silage review: Recent advances and future uses of silage additives (2018) · cites this paper as Hoffman et al., 2011
Experimental — accuracy not yet measuredThe cited source says what the claim attributes to it.
In the citing paper
“Proteolysis occurs naturally during ensiling (Hoffman et al., 2011), but it requires as much as 10 mo for the protein matrix to be hydrolyzed to an extent that optimizes starch availability in corn silage (Ferraretto et al., 2014).”
From Silage review: Recent advances and future uses of silage additives (2018) · cites this paper as Hoffman et al., 2011
In the citing paper
“However, in maize silages (both whole-plant maize and grain silages), proteolysis has been positively associated with starch digestibility due to the degradation of prolamins that surround the starch granules [40].”
From The Effects of Short-Time Delayed Sealing on Fermentation, Aerobic Stability and Chemical Composition on Maize Silages (2023) · cites this paper as [40]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Official Methods of Analysis of
Helrick, K. (1980) · Analytical Chemistry · doi:10.1021/ac50052a726 · Cited by 59,351
Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(91)78551-2 · Cited by 28,658
Littell, R. C. (1996) · Medical Entomology and Zoology · Cited by 10,345
Forage Fiber Analysis (Apparatus, reagents, procedures and some applications)
Goering, H. K. (1970) · Medical Entomology and Zoology · Cited by 5,738
Biochemistry and molecular biology of plants
Short, T. W. (2001) · Biochemistry and Molecular Biology Education · Cited by 3,833
Biochemistry & molecular biology of plants
(2001) · Choice Reviews Online · doi:10.5860/choice.38-3895 · Cited by 2,980
Near-Infrared Technology: In the Agricultural and Food Industries
Williams, P. C., & Norris, K. (2001) · Andalas University Repository (Andalas University) · Cited by 2,749
Limits to Starch Digestion in the Ruminant Small Intestine1,2
Owens, F. N., Zinn, R. A., & Kim, Y. K. (1986) · Journal of Animal Science · doi:10.2527/jas1986.6351634x · Cited by 573
Zein: A History of Processing and Use
Lawton, J. W. (2002) · Cereal Chemistry · doi:10.1094/cchem.2002.79.1.1 · Cited by 513
Proteolysis in plants: mechanisms and functions
Vierstra, R. D. (1996) · Plant Molecular Biology · doi:10.1007/bf00039386 · Cited by 386
Flagged first, then by citations
Research works printed but not linked: 2
- Allen, 2008, Endosperm type of dry ground corn grain affects ruminal and total tract digestion of starch in lactating dairy cows, J. Dairy Sci., 91, 529
- Newbold, 2006, Effect of storage time on ruminal starch degradability in corn silage, J. Dairy Sci., 89, 190
Cited by
Sorted by citations
Items 201–248 of 248
Fernandes, J. (2014) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/d.11.2014.tde-12082014-151639
Sawall, Z. J. (2015) · University of Minnesota Digital Conservancy (University of Minnesota)
Akins, Ferraretto, L. F., & SHAVER, R. D. (2012)
Self-citationSchönell, E. P. (2016) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/d.11.2016.tde-09112016-103351
Wyk, V., & María, A. (2017) · UpSpace Institutional Repository (University of Pretoria)
Miranda-Yuquilema, J. E., Marín-Cárdenas, A., González-Pérez, M., & Valla-Ceped, A. P. (2018) · The Biologist · doi:10.24039/rtb2018162246
da Silva, É. B. (2024) · Library, Museums and Press - UDSpace (University of Delaware) · doi:10.58088/8fsh-7y38
Efeito da ensilagem de milho na modulação da comunidade bacteriana endógena
Estrada, P. D. A. C. (2019) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/t.11.2019.tde-27032019-160240
dos Santos, W. P. (2019) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/t.11.2019.tde-12072019-150405
Salvo, P. A. R. (2019) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/t.11.2019.tde-12122019-161636
Wei, S. (2020) · Seoul National University Open Repository (Seoul National University)
Pereira, S. N., Viégas, J., Schumacher, L. L., Tonin, T. J., da Costa, L. L., Bender, A. B. B., & Jungbeck, M. (2021) · African Journal of Range and Forage Science · doi:10.2989/10220119.2021.1934120 · Cited by 1
Guimarães, G. S., Azevêdo, J. A. G., Cairo, F. C., da Silva, C. S., Souza, L. L., Nunes, F. D. S., de Carvalho, G. G. P., de Araújo, G. G. L., & Silva, R. R. (2021) · Research Square · doi:10.21203/rs.3.rs-985272/v1
What's New with Corn Silage for Dairy Cattle?
Ferraretto, L. F., & SHAVER, R. D. (2012)
Self-citationDondé, S. C. (2021) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/d.11.2021.tde-11022022-092541
Neto, Á. B. D. S. (2022) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/d.11.2022.tde-11042022-153734
Kirwan, S. F., Pierce, K. M., Serra, E., Gath, V., Rajauria, G., & Boland, T. M. (2022) · Ruminants · doi:10.3390/ruminants2020012
Ramirez, J. C. R., Olguin, J. L. L., Gutiérrez‐Leyva, R., Ulloa, J. A., Nunez, F. G., Rosas‐Ulloa, P., & Gurrola, A. G. (2022) · Animal Nutrition and Feed Technology · doi:10.5958/0974-181x.2022.00004.x
Bugoni, M. (2022) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/d.10.2022.tde-28072022-094201
Canbolat, Ö. (2023) · Gıda ve Yem Bilimi Teknolojisi Dergisi · doi:10.56833/bursagida.1279059 · Cited by 1
de Araújo, D. R., Gomes, F. A., Moreira, J. G. D. V., Mattar, E. P. L., de Araújo, E. A., Ferreira, J. B., de Souza, C. S., & Nascimento, L. D. O. (2023) · Ciência Animal Brasileira · doi:10.1590/1809-6891v24e-74403e
Detecting Differences in Starch Fermentation of Corn Hybrids Harvested at Silage MaturitiesPreprint
Schlau, N., Mertens, D. R., & Taysom, D. M. (2023) · SSRN Electronic Journal · doi:10.2139/ssrn.4641748
Schlau, N., Mertens, D. R., & Taysom, D. M. (2024) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2024.116036
Use of bacterial inoculant in rehydrated corn grain silage
Nunes, F. C., Guimarães, K. C., Abrão, F. O., Cunha, F. P., Pereira, V. C. R. D. S., Paim, T. D. P., & Fernandes, P. B. (2024) · Journal of Applied Animal Research · doi:10.1080/09712119.2024.2400534
da Silva, V. R., da Freiria, L. B., Negrão, F. D. M., Silva, P. I. J. L. D. R. E., Miranda, E. S., da Fonseca, A. R., dos Santos, L. R. A., & Pereira, T. B. (2024) · Caderno Pedagógico · doi:10.54033/cadpedv21n10-235
Tavares, B. C., Dias, M. K. R., Royer, J. L., Nascimento, V. A., Dias, F. J. D. S., Saenz, E. A. C., Câmara, L. R. D. A., & da Silveira, D. M. (2024) · Cuadernos de Educación y Desarrollo · doi:10.55905/cuadv16n11-073 · Cited by 1
Carbohydrate and protein composition and kinetic degradation of sorghum silage stored long-term
Pereira, S. N., Schumacher, L. L., Valle, T. A. D., Nemoto, B. D. S., Antunes, M. É. D. L., Selau, P. D. O., Skonieski, F. R., Tonin, T. J., & Viégas, J. (2025) · Ciência Rural · doi:10.1590/0103-8478cr20240156
Corn and sorghum ground or rehydrated and ensiled in feedlot cattle diets
Guimarães, L. A., Villela, S. D. J., Ezequiel, J. M. B., Martins, P. G. M. D. A., Costa, P. M., Leonel, F. D. P., Vítor, C. M. T., & Paschoaloto, J. R. (2025) · Revista Brasileira de Zootecnia · doi:10.37496/rbz5420230027
Corn Silage, Managing the Manageable
Mahanna, B., Zahorodnii, A., & Chernyuk, S. (2025) · Tehnologìâ virobnictva ì pererobki produktìv tvarinnictva · doi:10.33245/2310-9289-2025-194-1-72-80
Effect of mechanical processing and storage length on whole-plant sorghum silage fermentation
de Oliveira, M. H., Fernandes, T., de Souza, D. M., Barros, J. D. S., Meirelles, P. R. D. L., & Costa, C. (2025) · Anais da Academia Brasileira de Ciências · doi:10.1590/0001-3765202520241162
Cardoso, M. V. S. B., da Silva, Y. F. S., Sousa, M. P. R., Silva, L., Silva, C. F., Soares, F. E. F., & Ávila, C. L. D. S. (2025) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2025.116470
Lang, C., Mahmood, M., Khiaosa‐ard, R., Kaltenegger, A., Humer, E., Wetzels, S. U., Gross, J. J., Robert, C. A. M., Mateo, P., Erb, M., et al. (2025) · Journal of Animal Physiology and Animal Nutrition · doi:10.1111/jpn.70006
Silva, W. D. R., Silva, A. N. D., Tiengo, M. A., Rezende, J. P. A., de Souza, S. P., Pereira, R. A. N., DeVries, T. J., & Pereira, M. N. (2025) · Journal of Dairy Science · doi:10.3168/jds.2025-26966
Durman, T., Bueno, A. V. I., Jobim, C. C., de Souza, A. M., Horst, E. H., Cesar, P. V. P., Ienke, L. A., Mokochinski, N. S., Giacomet, J. A. D. A., & Neumann, M. (2025) · Semina Ciências Agrárias · doi:10.5433/1679-0359.2025v46n5p1629 · Cited by 1
de Melo, N. N., Cordeiro, M. W. S., & Bernardes, T. F. (2025) · The Journal of Agricultural Science · doi:10.1017/s0021859625100403
Liu, X., Romero, N., Cronin, S., da Silva, E. B., Kung, L., & Gressley, T. F. (2025) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2025.116603 · Cited by 1
Gao, Y., Yao, K., Song, L., Jiang, W., Yang, M., Zhou, Y., Abduali, S., Abdurayimu, K., Shao, W., Yang, L., et al. (2026) · Frontiers in Veterinary Science · doi:10.3389/fvets.2025.1688465
Gandra, J. R., Gandra, É. R. S., Pedrini, C. A., de Góes, R. H. D. T. E. B., Oliveira, E. R., Takiya, C. S., Valle, T. A. D., Vasconcelos, W. R. F., Rosa, K. P., & Ribeiro, B. V. (2026) · New Zealand Journal of Agricultural Research · doi:10.1002/jag2.70060
Saray, A. A., ROUZBEHAN, Y., Rezaei, J., & Hamidi, B. A. (2026) · Journal of Animal Science · doi:10.1093/jas/skag147
A review on quality changes in total mixed fermentation during storage periods
Lee, Y.-S., & Chu, G. M. (2026) · Food Preservation and Processing Industry · doi:10.11002/fppi.2026.25.1.42
Daniel, J. L. P., Bragatto, J. M., & Carvalho, M. G. M. (2026) · Journal of Dairy Science · doi:10.3168/jds.2026-28813
Essential oil compounds as strategic additives for rehydrated corn grain silage
da Silva, Á. B., Morais, G., Ribeiro, A. P., Andrade, A. V., Gasparina, J. M., Wang, C., Ferreira, E. M., & Nussio, L. G. (2026) · Revista Brasileira de Zootecnia · doi:10.37496/rbz5520250208
Marques, J. D. D. C., Pozada, T. N., Rediss, W. B., Falson, J. P. S., da Silva, N. C., Schafhäuser, J., & Rabêlo, C. H. S. (2026) · Revista Brasileira de Saúde e Produção Animal · doi:10.1590/20250035
Lopes, J., Arcari, M., Vieira, P., Martins, C., Alves, B., Aleri, J. W., dos Santos, M. V., & Sousa, D. (2026) · SSRN Electronic Journal · doi:10.2139/ssrn.7498126
Metadata
- Authors
- Hoffman, P. C., Esser, N. M., Shaver, R. D., Coblentz, W. K., Scott, M. P., Bodnar, A. L., Schmidt, R. J., & Charley, R. C.
- Affiliations
- University of Wisconsin–Madison (US)
- U.S. Dairy Forage Research Center (US)
- Agricultural Research Service (US)
- Iowa State University (US)
- Corresponding author
- Hoffman, P.C.
- Venue
- Journal of Dairy Science
- Publisher
- American Dairy Science Association
- Published
- 2011-05
- Volume
- 94
- Issue
- 5
- Pages
- 2465-2474
- ISSN
- 0022-0302 (ISSN-L)
- OpenAlex
- W1979239946
- Cited by
- 249
- References
- 30
- Citations by year
- 2026: 11 · 2025: 18 · 2024: 18 · 2023: 25 · 2022: 22 · 2021: 25 · 2020: 24 · 2019: 22 · 2018: 17 · 2017: 9 · 2016: 15 · 2015: 9 · 2014: 17 · 2013: 5 · 2012: 12
- FWCI
- 20.71
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Dairy Science (publishedVersion)
- PubMed (publishedVersion)
- Lincoln (University of Nebraska) (submittedVersion)
- Iowa State University Digital Repository (Iowa State University) (submittedVersion)
- Funders
- University of Wisconsin-Madison
- Topics
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Crop Yield and Soil Fertility
- Keywords
- high-moisture corn, ensiling time, zein, vitreous endosperm, protein degradation, ammonia nitrogen, fermentation products, lactate, acetate, maize hybrids, silage
- MeSH terms
- Animals, Animal Nutritional Physiological Phenomena, Cattle, Zea mays, Dietary Proteins, Digestion, Lactobacillus, Rumen, Silage, Starch, Time Factors, Zein
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
