Short communication: A study of Lactobacillus isolates' adherence to and influence on membrane integrity of human Caco-2 cells
Open accessAbstract
The selection criteria of ideal probiotic bacteria are complex and involve many factors. One key criterion is based on the ability of the probiotic bacteria to adhere to the epithelial lining of the gastrointestinal tract. The objective of this study was to evaluate and compare the adherence and influence on membrane integrity of 2 selected lactobacilli isolates-Lactobacillus rhamnosus MI13 (dairy food origin) and L. plantarum RC2 (bovine rumen origin)-to Caco-2 cells in the presence and absence of Escherichia coli. The adhesion and influence on membrane integrity properties of the 2 Lactobacillus isolates were compared with Escherichia coli, a human commensal bacterium. From the adhesion studies, we concluded that the bovine rumen isolate exhibited better adherence to Caco-2 cells than the dairy food isolate. In contrast, the dairy food isolate better protected the Caco-2 monolayer from damage induced by ethanol.
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Good correlation between in vitro adhesion using Caco-2 and HT-29 cell lines and in vivo adhesion has been demonstrated in numerous studies.
“Good correlation between adhesion carried out in vitro, applying Caco-2 and HT-29 cell lines and adhesion in vivo, has been demonstrated in numerous studies conducted (Nowak and Motyl, 2017; Jose et al. 2017).”
From Adhesion and aggregation properties of Lactobacillaceae strains as protection ways against enteropathogenic bacteria (2022) · cites this paper as Jose et al. 2017
The Short Communication section describes Caco-2 cells as a widely used in vitro model for studying bacterial interactions with the human gut. It does not report a demonstrated correlation between in vitro and in vivo adhesion, and its adhesion experiments use Caco-2 cells, not HT-29 cells. No page number is available in our copy. (LLM, conf=0.82)
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- Authors
- Jose, N. M., Bunt, C. R., McDowell, A., Chiu, J. Z. S., & Hussain, M. A.
- Affiliations
- Lincoln University (NZ)
- University of Otago (NZ)
- Corresponding author
- Bunt, Craig R.
- Hussain, Malik A.
- Venue
- Journal of Dairy Science
- Publisher
- American Dairy Science Association
- Published
- 2017-10
- Volume
- 100
- Issue
- 10
- Pages
- 7891-7896
- ISSN
- 0022-0302 (ISSN-L)
- OpenAlex
- W2746831850
- Cited by
- 17
- References
- 19
- Citations by year
- 2026: 1 · 2025: 2 · 2023: 1 · 2022: 4 · 2021: 3 · 2020: 1 · 2019: 2 · 2018: 3
- FWCI
- 1.85
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Dairy Science (publishedVersion)
- PubMed (publishedVersion)
- Lincoln University Research Archive (Lincoln University) (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Keywords
- Lactobacillus rhamnosus, Lactobacillus plantarum, probiotic bacteria, bacterial adhesion, Caco-2 cells, intestinal epithelial barrier, membrane integrity, Escherichia coli, probiotic selection criteria
- MeSH terms
- Animals, Bacterial Adhesion, Cattle, Cell Membrane, Escherichia coli, Humans, Rumen, Caco-2 Cells, Probiotics, Cell Membrane Structures, Lactiplantibacillus plantarum, Lacticaseibacillus rhamnosus
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