Assessing the adhesion of putative indigenous probiotic lactobacilli to human colonic epithelial cells
Open accessAbstract
Background & objectives: Adherence of bacteria to epithelial cells and mucosal surfaces is a key criterion for selection of probiotic. We assessed the adhesion property of selected indigenous probiotic Lactobacillus strains based on their hydrophobicity and ability to adhere to human epithelial cells. Methods: Five human faecal Lactobacillus isolates, one from buffalo milk and one from cheese were assessed for hydrophobicity following the microbial adhesion to hydrocarbons (MATH) method and colonization potentials based on their adherence to Caco2 and HT-29 colonic adenocarcinomal human intestinal epithelial cell lines. Lactobacillus strains that adhered to Caco2 and HT-29 cell lines were quantified by plating after trypsinization and simultaneously the adhered bacteria were also examined microscopically after staining with Geimsa stain and counted in different fields. Results: Among the tested faecal isolates, L. plantarum Lp91 showed maximum percentage hydrophobicity (35.73±0.40 for n-hexadecane and 34.26±0.63 for toluene) closely followed by L. plantarum Lp9 (35.53±0.29 for n-hexadecane and 33.00±0.57 for toluene). Based on direct adhesion to epithelial cells, L. plantarum Lp91 was the most adhesive strain to HT-29 and Caco2 cell lines with per cent adhesion values of 12.8 ± 1.56 and 10.2 ± 1.09, respectively. L. delbrukeii CH4, was the least adhesive with corresponding figures of 2.5 ± 0.37 and 2.6 ± 0.20 per cent on HT-29 and Caco2 cell lines. Adhesion of the six isolated Lactobacillus strain to HT-29 cell and Caco2 lines as recorded under microscope varied between 131.0 ± 13.9 (Lp75) to 342.7 ± 50.52 (Lp91) and 44.7 ± 9.29 (CH4) to 315.7± 35.4 (Lp91), respectively. Interpretation & conclusions: Two Indigenous probiotic Lactobacillus strains (Lp9, Lp91) demonstrated their ability to adhere to epithelial cell and exhibited strong hydrophobicity under in vitro conditions, and thus could have better prospects to colonize the gut with extended transit
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Caco-2 cells express morphological and functional differentiation in vitro and show characteristics of mature enterocytes; HT-29 cells show typical epithelial cell morphology and produce large amounts of mucus.
“The Caco-2 cell line express morphological and functional differentiation in vitro and show characteristics of mature enterocytes. In turn, the HT-29 line shows a typical epithelial cell morphology, producing large amounts of mucus (Chauviere et al. 1992; Duary et al. 2011; Sharma and Kanwar 2017).”
From Adhesion and aggregation properties of Lactobacillaceae strains as protection ways against enteropathogenic bacteria (2022) · cites this paper as Duary et al. 2011
The paper’s introductory passage describes Caco-2 and HT-29 cells as having structural and functional features of normal human enterocytes. Materials & Methods identifies HT-29 as mucus-secreting. It does not specify that HT-29 produces large amounts of mucus or explicitly describe its morphology as typical epithelial morphology; page numbers are unavailable in our copy. (LLM, conf=0.86)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Microbes in Gastrointestinal Health and Disease
Neish, A. S. (2008) · Gastroenterology · doi:10.1053/j.gastro.2008.10.080 · Cited by 1,388 · see citing works
Genes and Molecules of Lactobacilli Supporting Probiotic Action
Lebeer, S., Vanderleyden, J., & De Keersmaecker, S. C. J. (2008) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00017-08 · Cited by 1,013 · see citing works
Jacobsen, C. N., Nielsen, V. R., Hayford, A. E., Møller, P. L., Michaelsen, K. F., Pærregaard, A., Sandström, B., Tvede, M., & Jakobsen, M. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.11.4949-4956.1999 · Cited by 880 · see citing works
Bernet‐Camard, M., Brassart, D., Neeser, J., & Servin, A. L. (1994) · Gut · doi:10.1136/gut.35.4.483 · Cited by 751 · see citing works
Survival of Lactic Acid Bacteria in the Human Stomach and Adhesion to Intestinal Cells
Conway, P. L., Gorbach, S. L., & Goldin, B. R. (1987) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(87)79974-3 · Cited by 702 · see citing works
Adhesion of some probiotic and dairy Lactobacillus strains to Caco-2 cell cultures
Tuomola, E., & Salminen, S. (1998) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(98)00033-6 · Cited by 396 · see citing works
In vitro fermentation of cereal dietary fibre carbohydrates by probiotic and intestinal bacteria
Crittenden, R. G., Karppinen, S., Ojanen, S., Tenkanen, M., Fagerström, R., Mättö, J., Saarela, M., Mattila, T., & Poutanen, K. S. (2002) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.1095 · Cited by 339 · see citing works
Pretzer, G., Snel, J., Molenaar, D., Wiersma, A., Bron, P. A., Lambert, J., de Vos, W. M., van der Meer, R., Smits, M. A., & Kleerebezem, M. (2005) · Journal of Bacteriology · doi:10.1128/jb.187.17.6128-6136.2005 · Cited by 316 · see citing works
Adhesion of human Lactobacillus acidophilus strain LB to human enterocyte-like Caco-2 cells
CHAUVIERE, G., COCONNIER, M.-H., KERNEIS, S., FOURNIAT, J., & SERVIN, A. L. (1992) · Journal of General Microbiology · doi:10.1099/00221287-138-8-1689 · Cited by 249 · see citing works
Boonaert, C. J. P., & Rouxhet, P. G. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.6.2548-2554.2000 · Cited by 243 · see citing works
Elo, S., Saxelin, M., & Salminen, S. (1991) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.1991.tb00595.x · Cited by 151 · see citing works
Geertsema‐Doornbusch, G. I., van der Mei, H. C., & Busscher, H. J. (1993) · Journal of Microbiological Methods · doi:10.1016/0167-7012(93)90072-p · Cited by 137 · see citing works
Adherence of Lactobacillus Species to Human Fetal Intestinal Cells
Kleeman, E., & Klaenhammer, T. R. (1982) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(82)82462-4 · Cited by 115 · see citing works
Hemagglutination, Adherence, and Surface Properties of Vaginal Lactobacillus Species
Andreu, A., Stapleton, A. E., Fennell, C. L., Hillier, S. L., & Stamm, W. E. (1995) · The Journal of Infectious Diseases · doi:10.1093/infdis/171.5.1237 · Cited by 94 · see citing works
Tuomola, E., Ouwehand, A. C., & Salminen, S. (1999) · Letters in Applied Microbiology · doi:10.1046/j.1365-2672.1999.00518.x · Cited by 83 · see citing works
Wang, B., Wei, H., Yuan, J., Li, Q., Li, Y., Li, N., & Li, J. (2008) · Current Microbiology · doi:10.1007/s00284-008-9148-2 · Cited by 77 · see citing works
Electrophoretic Mobility Distributions of Single-Strain Microbial Populations
van der Mei, H. C., & Busscher, H. J. (2001) · Applied and Environmental Microbiology · doi:10.1128/aem.67.2.491-494.2001 · Cited by 67 · see citing works
A model study of factors involved in adhesion of Pseudomonas fluorescens to meat
Piette, J. P. G., & Idziak, E. S. (1992) · Applied and Environmental Microbiology · doi:10.1128/aem.58.9.2783-2791.1992 · Cited by 65 · see citing works
Moussavi, M., & Adams, M. C. (2009) · Current Microbiology · doi:10.1007/s00284-009-9545-1 · Cited by 53 · see citing works
Sarem, F., Sarem-Damerdji, L.-O., & Nicolas, J. P. (1996) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.1996.tb01198.x · Cited by 50 · see citing works
Physicochemical Surface Characteristics of Urogenital and Poultry Lactobacilli
Cuperus, P. L., van der Mei, H. C., Reid, G., Bruce, A. W., Khoury, A., Rouxhet, P. G., & Busscher, H. J. (1993) · Journal of Colloid and Interface Science · doi:10.1006/jcis.1993.1118 · Cited by 48 · see citing works
[13] Microbial interactions to intestinal mucosal models
Ouwehand, A. C., Tuomola, E., Lee, Y. K., et al. (2001) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(01)37015-5 · Cited by 37 · see citing works
Adhesion of Two Lactobacillus gasseri Probiotic Strains on Caco-2 Cells
Narat, M., Zorič, M., & Matijašić, B. B. (2003) · DOAJ (DOAJ: Directory of Open Access Journals) · Cited by 33 · see citing works
Cuperus, P. L., der Mei, H. C. V., Reid, G., et al. (1995) · PubMed · Cited by 14 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Adhesion abilities of dairy Lactobacillus plantarum strains showing an aggregation phenotype
García-Cayuela, T., Korany, A. M., Bustos, I., P. Gómez de Cadiñanos, L., Requena, T., Peláez, C., & Martínez-Cuesta, M. C. (2014) · Food Research International · doi:10.1016/j.foodres.2014.01.010 · Cited by 286 · see citing works
Pirbaglou, M., Katz, J., de Souza, R. J., Stearns, J. C., Motamed, M., & Ritvo, P. (2016) · Nutrition Research · doi:10.1016/j.nutres.2016.06.009 · Cited by 270 · see citing works
Han, Q., Kong, B., Chen, Q., Sun, F., & Zhang, H. (2017) · Journal of Functional Foods · doi:10.1016/j.jff.2017.03.020 · Cited by 180 · see citing works
In vitro probiotic properties of Lactobacillus fermentum SK5 isolated from vagina of a healthy woman
Kaewnopparat, S., Dangmanee, N., Kaewnopparat, N., Srichana, T., Chulasiri, M., & Settharaksa, S. (2013) · Anaerobe · doi:10.1016/j.anaerobe.2013.04.009 · Cited by 126 · see citing works
Zawistowska-Rojek, A., Kośmider, A., Stępień, K., & Tyski, S. (2022) · Archives of Microbiology · doi:10.1007/s00203-022-02889-8 · Cited by 125 · see citing works
“The most commonly used in adherence in vitro models, structurally and functionally similar to human enterocytes are colon adenocarcinoma cells (Duary et al. 2011).”
BackgroundEffects of probiotics on the content and bioaccessibility of phenolic compounds in red pitaya pulp
Morais, S. G. G., da Silva Campelo Borges, G., dos Santos Lima, M., Martín-Belloso, O., & Magnani, M. (2019) · Food Research International · doi:10.1016/j.foodres.2019.108681 · Cited by 109 · see citing works
Cozzolino, A., Vergalito, F., Tremonte, P., Iorizzo, M., Lombardi, S. J., Sorrentino, E., Luongo, D., Coppola, R., Di Marco, R., & Succi, M. (2020) · Microorganisms · doi:10.3390/microorganisms8020189 · Cited by 84 · see citing works
Sharma, S., & Kanwar, S. S. (2017) · Journal of Food Science and Technology · doi:10.1007/s13197-017-2807-1 · Cited by 46 · see citing works
Jose, N. M., Bunt, C. R., McDowell, A., Chiu, J. Z. S., & Hussain, M. A. (2017) · Journal of Dairy Science · doi:10.3168/jds.2017-12912 · Cited by 17 · see citing works
- Authors
- Duary, R. K., Rajput, Y. S., Batish, V. K., & Grover, S.
- Affiliations
- Indian Council of Agricultural Research (IN)
- National Dairy Research Institute (IN)
- Corresponding author
- Grover, Sunita
- Venue
- Indian Journal of Medical Research
- Publisher
- Scientific Scholar
- Published
- 2011-11
- Volume
- 134
- Issue
- 5
- Pages
- 664-671
- ISSN
- 0971-5916 (ISSN-L)
- PMC
- PMC3249965
- OpenAlex
- W2160595607
- Cited by
- 242
- References
- 24
- Citations by year
- 2026: 10 · 2025: 10 · 2024: 14 · 2023: 22 · 2022: 18 · 2021: 21 · 2020: 13 · 2019: 20 · 2018: 27 · 2017: 31 · 2016: 15 · 2015: 19 · 2014: 10 · 2013: 9 · 2012: 3
- FWCI
- 10.28
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- The Indian Journal of Medical Research (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- probiotic Lactobacillus, bacterial adhesion, hydrophobicity, colonic epithelial cells, Caco-2 cells, HT-29 cells, Lactobacillus plantarum, Lactobacillus delbrueckii, fecal isolates, probiotic strain selection, breast milk, cheese, colonization potential, buffalo milk
- MeSH terms
- Bacterial Adhesion, Colon, Epithelial Cells, Feces, Humans, Hydrocarbons, Intestines, Caco-2 Cells, HT29 Cells, Probiotics, Gastrointestinal Tract, Lactiplantibacillus plantarum, Hydrophobic and Hydrophilic Interactions
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
