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The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.

Multisite Validation of Cryptococcal Antigen Lateral Flow Assay and Quantification by Laser Thermal Contrast

Open access
Boulware, David R., Rolfes, Melissa A., Rajasingham, Radha, von Hohenberg, Maximilian, Qin, Zhenpeng, Taseera, Kabanda, Schutz, Charlotte, Kwizera, Richard, Butler, Elissa K., Meintjes, Graeme, Muzoora, Conrad, Bischof, John C., Meya, David B.·2014·Emerging Infectious Diseases

Abstract

Cryptococcal meningitis is common in sub-Saharan Africa. Given the need for data for a rapid, point-of-care cryptococcal antigen (CRAG) lateral flow immunochromatographic assay (LFA), we assessed diagnostic performance of cerebrospinal fluid (CSF) culture, CRAG latex agglutination, India ink microscopy, and CRAG LFA for 832 HIV-infected persons with suspected meningitis during 2006-2009 (n = 299) in Uganda and during 2010-2012 (n = 533) in Uganda and South Africa. CRAG LFA had the best performance (sensitivity 99.3%, specificity 99.1%). Culture sensitivity was dependent on CSF volume (82.4% for 10 μL, 94.2% for 100 μL). CRAG latex agglutination test sensitivity (97.0%-97.8%) and specificity (85.9%-100%) varied between manufacturers. India ink microscopy was 86% sensitive. Laser thermal contrast had 92% accuracy (R = 0.91, p<0.001) in quantifying CRAG titers from 1 LFA strip to within <1.5 dilutions of actual CRAG titers. CRAG LFA is a major advance for meningitis diagnostics in resource-limited settings.

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Crossref · 10.3201/eid2001.130906

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Research works printed but not linked: 2

  1. Jarvis, 2012, Cryptococcal antigen screening and preemptive therapy in patients initiating antiretroviral therapy in resource-limited settings: a proposed algorithm for clinical implementation., J Int Assoc Physicians AIDS Care (Chic), 11, 374, 10.1177/1545109712459077 · doi:10.1177/1545109712459077
  2. Gates-Hollingsworth, 2013, Serotype sensitivity of a lateral flow immunoassay for cryptococcal antigen., Clin Vaccine Immunol, 20, 634, 10.1128/CVI.00732-12 · doi:10.1128/cvi.00732-12

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Metadata

Authors
Boulware, D. R., Rolfes, M. A., Rajasingham, R., von Hohenberg, M., Qin, Z., Taseera, K., Schutz, C., Kwizera, R., Butler, E. K., Meintjes, G., Muzoora, C., Bischof, J. C., & Meya, D. B.
Venue
Emerging Infectious Diseases
Published
2014-01
Volume
20
Issue
1
Pages
45-53
ISSN
  • 1080-6040 (ISSN-L)
  • 1080-6059
OpenAlex
W1976256249
Cited by
321
References
34
FWCI
13.84
Type
journal article
Language
en
Open access
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CoChat. (2026). Citation check: Multisite Validation of Cryptococcal Antigen Lateral Flow Assay and Quantification by Laser Thermal Contrast. https://app.cochat.ai/papers/10.3201/eid2001.130906
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Boulware, D. R., Rolfes, M. A., Rajasingham, R., von Hohenberg, M., Qin, Z., Taseera, K., Schutz, C., Kwizera, R., Butler, E. K., Meintjes, G., Muzoora, C., Bischof, J. C., & Meya, D. B. (2014). Multisite Validation of Cryptococcal Antigen Lateral Flow Assay and Quantification by Laser Thermal Contrast. Emerging Infectious Diseases. https://doi.org/10.3201/eid2001.130906

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