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Cited by 29Bacterial biofilms and quorum sensingEssential Oils and Antimicrobial ActivityMedicinal Plant Research

Evaluation of anti-biofilm, anti-quorum, anti-dysenteric potential of designed polyherbal formulation: In vitro and in vivo study

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Singh, Devendra, Singh, Virendra, Bhushan Mishra, Shanti, Sharma, Deepmala, Agarwal, Vishnu·2022·Journal of Applied Biomedicine
DOI 10.32725/jab.2022.005
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Abstract

Bacillary dysentery (shigellosis) continues to cause havoc worldwide, with a high infectivity rate. It causes bloody diarrhea, and around 99% of bacillary dysentery cases occur in developing countries. The objective of this study is to develop a polyherbal formulation with the scientific rationale in treating infectious bacillary dysentery disease. The anti-bacterial activity, the minimum inhibitory concentration of the formulation against bacillary dysentery, causing microbes like Shigella flexneri (MTCC 1457), Escherichia coli (MTCC 1687), and Salmonella enterica (MTCC 98), was analysed by well-diffusion method and broth dilution method, respectively. The biofilm inhibition activity was determined on 96 well polystyrene plates and anti-quorum sensing activity by Chromobacterium violaceum CV026. The cytotoxicity was examined by acute oral toxicity. Excreta and organ bacterial load were analyzed by serial dilution method. The formulation efficacy was determined by analyzing the blood sample of rats. The antimicrobial efficacy of the developed formulation was calculated by measuring the zone of inhibition which was found to be 24 mm, 25 mm, and 25 mm, and the MIC values of 1.5 mg/ml, 1.5 mg/ml, and 2.0 mg/ml against S. flexneri, S. enterica, E. coli, respectively. The results show that the polyherbal formulation significantly reduced biofilm formation and has anti-quorum sensing activity. The formulation also effectively decreases the bacterial load and increases the K+, Na+, and Ca++ ions in animals treated with the formulation. The developed formulation was found to be non-toxic and effective against bacillary dysentery; thus, it can be used for treating bacillary dysentery and related complications.

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  • The main mechanisms of action of probiotics include advancement of the epithelial barrier, increased adhesion to the intestinal mucosa, production of antibacterial substances, competitive exclusion of pathogens, simultaneous suppression of bacterial adhesion, and modulation of the immune system.

    “According to data in the literature, the advancement of the epithelial barrier, increased adhesion to the intestinal mucosa, production of antibacterial substances, competitive exclusion of pathogens, simultaneous suppression of bacterial adhesion, and modulation of the immune system are some of the main mechanisms of action of probiotics [87,88,89,90,91] (Figure 1).”

    From The Potential Impact of Probiotics on Human Health: An Update on Their Health-Promoting Properties (2024) · cites this paper as [91]

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Research works printed but not linked: 2

  1. CLSI -Clinical and Laboratory Standards Institute (2006). Methods for dilution antimicrobial susceptibilty tests for bacteria that grow aerobically. Approved Standard (7th ed.) Clinical and Laboratory Standards Institute document M7-A7.
  2. Niyogi SK (2005). Shigellosis. J Microbiol 43(2): 133-143.

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Authors
Singh, D., Singh, V., Bhushan Mishra, S., Sharma, D., & Agarwal, V.
Affiliations
  • Motilal Nehru National Institute of Technology (IN)
  • Maulana Azad Medical College (IN)
  • National Institute of Technology Raipur (IN)
Corresponding author
Agarwal, Vishnu
ORCID
Venue
Journal of Applied Biomedicine
Publisher
University of South Bohemia in Ceske Budejovice
Published
2022-03-17
Volume
20
Issue
1
Pages
7-14
ISSN
  • 1214-021X (ISSN-L)
  • 1214-0287
DOI
10.32725/jab.2022.005
OpenAlex
W4220854622
Cited by
29
References
31
Citations by year
2026: 5 · 2025: 1 · 2024: 14 · 2023: 8 · 2022: 1
FWCI
1.96
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Applied Biomedicine (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
Motilal Nehru National Institute of Technology Allahabad
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
Keywords
shigellosis, Shigella flexneri, polyherbal formulation, biofilm inhibition, anti-quorum sensing, antibacterial activity, Escherichia coli, Salmonella enterica, acute oral toxicity, bacterial load, electrolyte concentration, herbal medicine
MeSH terms
Animals, Dysentery, Bacillary, Escherichia coli, Microbial Sensitivity Tests, Biofilms, Rats, Quorum Sensing

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