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Cited by 27Essential Oils and Antimicrobial ActivityAntimicrobial agents and applicationsBacterial biofilms and quorum sensing

Screening of antimicrobial, anti-quorum sensing activity and cytotoxicity of origanumoil against Gram-positive and Gram-negative bacteria

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Devendra Singh, DevendraX, Agarwal, Vishnu·2021·Biomedicine
DOI 10.51248/.v41i3.1200
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Abstract

Introduction and Aim: Essential oils have been used from ancient times to treat different gram-positive and gram-negative bacterial-related infections. The study aims to screen the antibacterial, anti-quorum sensing activity of origanum oil against the common infection causing gram-negative and gram-positive bacteria. Material andMethods: The MIC (minimum inhibitory concentration) and antibacterial activity of origanum oil against the eight bacterial species, namely, Pseudomonas aeruginosa, Shigella flexneri, Salmonella enterica, Klebsiella pneumoniae, Streptococcus pneumonia, Streptococcus pyogenes, Staphylococcus aureus, and Enterococcus faecalis, was assessed by broth-dilution method and well diffusion method. The anti-quorum sensing activity was analyzed using bioreporter strain CV026 at sub-MIC concentrations, while the cytotoxicity of the origanum oil was analyzed using the SW480 cell line. Results: The oil's antibacterial activity was analyzed by measuring the clear inhibitory zone diameter, and maximum inhibitory zone of 36.66 mm zone against Staphylococcus aureus (Gram-positive) and 33.33 mm against Salmonella enterica (Gram-negative) of origanum oil was measured. And the minimum inhibitory zone of 16.66 mm zone of Enterococcus faecalis (Gram-positive) and 19.66 mm against Klebsiella pneumoniae (Gram-negative) of oil was noted. And the lowest MIC (0.1 mg/ml) of oilwas found against Streptococcus pneumonia. The oil significantly inhibited the violacein pigment production (30.29 %) at 0.02 mg/ml concentration (this oil concentration did not significantly affect the growth curve). The in vitro cytotoxicity assay shows that the oil inhibited the SW480 cells growth with increasing concentration. Conclusion: The origanum oil possesses antibacterial and anti-quorum sensing activity and can be used as an alternative for treating tested bacterial infection.

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  • The main mechanisms of action of probiotics include advancement of the epithelial barrier, increased adhesion to the intestinal mucosa, production of antibacterial substances, competitive exclusion of pathogens, simultaneous suppression of bacterial adhesion, and modulation of the immune system.

    “According to data in the literature, the advancement of the epithelial barrier, increased adhesion to the intestinal mucosa, production of antibacterial substances, competitive exclusion of pathogens, simultaneous suppression of bacterial adhesion, and modulation of the immune system are some of the main mechanisms of action of probiotics [87,88,89,90,91] (Figure 1).”

    From The Potential Impact of Probiotics on Human Health: An Update on Their Health-Promoting Properties (2024) · cites this paper as [88]

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Authors
Devendra Singh, D., & Agarwal, V.
ORCID
Agarwal, Vishnu
Venue
Biomedicine
Publisher
Indian Association of Biomedical Scientists
Published
2021-10-25
Volume
41
Issue
3
Pages
599-603
ISSN
0970-2067 (ISSN-L)
DOI
10.51248/.v41i3.1200
OpenAlex
W3209665266
Cited by
27
References
17
Citations by year
2026: 1 · 2024: 16 · 2023: 8 · 2022: 2
FWCI
3.89
Type
journal article
Language
en
Open access
Licence
Locations
Biomedicine (publishedVersion, cc-by)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Physical Sciences › Chemistry › Organic Chemistry › Antimicrobial agents and applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
Keywords
essential oils, antibacterial activity, anti-quorum sensing, cytotoxicity, Gram-positive bacteria, Gram-negative bacteria, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae, Klebsiella pneumoniae, Salmonella enterica, Shigella flexneri, Enterococcus faecalis, violacein production, minimum inhibitory concentration, SW480 cells

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