Rapid Detection and Identification of Wuchereria bancrofti, Brugia malayi, B. pahangi, and Dirofilaria immitis in Mosquito Vectors and Blood Samples by High Resolution Melting Real-Time P
Open accessAbstract
A simple, rapid, and high-throughput method for detection and identification of Wuchereria bancrofti, Brugia malayi, Brugia pahangi, and Dirofilaria immitis in mosquito vectors and blood samples was developed using a real-time PCR combined with high-resolution melting (HRM) analysis. Amplicons of the 4 filarial species were generated from 5S rRNA and spliced leader sequences by the real-time PCR and their melting temperatures were determined by the HRM method. Melting of amplicons from W. bancrofti, B. malayi, D. immitis, and B. pahangi peaked at 81.5±0.2℃, 79.0±0.3℃, 76.8±0.1℃, and 79.9±0.1℃, respectively. This assay is relatively cheap since it does not require synthesis of hybridization probes. Its sensitivity and specificity were 100%. It is a rapid and technically simple approach, and an important tool for population surveys as well as molecular xenomonitoring of parasites in vectors.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Screening for Dirofilaria spp. using PCR showed a sensitivity and specificity of 100% in various studies.
“Screening for Dirofilaria spp. using PCR showed a sensitivity and specificity of 100% in various studies [43,44,45].”
From The Incidence of Dirofilaria immitis in Shelter Dogs and Mosquitoes in Austria (2021) · cites this paper as [44]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Product Differentiation by Analysis of DNA Melting Curves during the Polymerase Chain Reaction
Ririe, K. M., Rasmussen, R. P., & Wittwer, C. T. (1997) · Analytical Biochemistry · doi:10.1006/abio.1996.9916 · Cited by 1,663 · see citing works
High-Resolution Genotyping by Amplicon Melting Analysis Using LCGreen
Wittwer, C. T., Reed, G. H., Gundry, C. N., Vandersteen, J. G., & Pryor, R. J. (2003) · Clinical Chemistry · doi:10.1373/49.6.853 · Cited by 1,259 · see citing works
Human and animal dirofilariasis: The emergence of a zoonotic mosaic
Simón, F., Siles-Lucas, M., Morchón, R., González-Miguel, J., Mellado, I., Carretón, E., & Montoya-Alonso, J. A. (2012) · Clinical Microbiology Reviews · doi:10.1128/cmr.00012-12 · Cited by 834 · see citing works
Editorial: The Global Programme to Eliminate Lymphatic Filariasis
Ottesen, E. A. (2000) · Tropical Medicine & International Health · doi:10.1046/j.1365-3156.2000.00620.x · Cited by 571 · see citing works
Diagnostic tools for filariasis elimination programs
Weil, G. J., & Ramzy, R. M. R. (2006) · Trends in Parasitology · doi:10.1016/j.pt.2006.12.001 · Cited by 232 · see citing works
Recommendations of the International Task Force for Disease Eradication
(1993) · PsycEXTRA Dataset · doi:10.1037/e546702006-001 · Cited by 212 · see citing works
Lymphatic filariasis: the disease and its control
Whitworth, J. A. (1993) · Transactions of the Royal Society of Tropical Medicine and Hygiene · doi:10.1016/0035-9203(93)90323-i · Cited by 148 · see citing works
Harlan, H. J. (1994) · Journal of Medical Entomology · doi:10.1093/jmedent/31.4.635 · Cited by 138 · see citing works
Brugian Filariasis: Epidemiological and Experimental Studies
Denham, D. A., & McGreevy, P. B. (1977) · Advances in Parasitology/Advances in parasitology · doi:10.1016/s0065-308x(08)60530-8 · Cited by 118 · see citing works
Morphology of the Larval Stages of Dirofilaria immitis in the Dog
Orihel, T. C. (1961) · Journal of Parasitology · doi:10.2307/3275301 · Cited by 97 · see citing works
A multiplex PCR for the simultaneous detection of species of filarioids infesting dogs
Latrofa, M. S., Weigl, S., Dantas-Torres, F., Annoscia, G., Traversa, D., Brianti, E., & Otranto, D. (2012) · Acta Tropica · doi:10.1016/j.actatropica.2012.01.006 · Cited by 70 · see citing works
Wongkamchai, S., Monkong, N., Mahannol, P., Taweethavonsawat, P., Loymak, S., & Foongladda, S. (2012) · Vector-Borne and Zoonotic Diseases · doi:10.1089/vbz.2012.0971 · Cited by 47 · see citing works
[Detection of Dirofilaria in Austrian dogs].
Duscher, G. G., Feiler, A., Wille-Piazzai, W., Bakonyi, T., Leschnik, M. W., Miterpáková, M., Kolodziejek, J., Nowotny, N. R., & Joachim, A. (2009) · PubMed · Cited by 38 · see citing works
Zoonotic Echinostome Infections in Free-Grazing Ducks in Thailand
Saijuntha, W., Duenngai, K., & Tantrawatpan, C. (2013) · Korean Journal of Parasitology · doi:10.3347/kjp.2013.51.6.663 · Cited by 35 · see citing works
Dirofilaria Repens from the Eyelid of a Woman in Thailand
Jariya, P., & Sucharit, S. (1983) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.1983.32.1456 · Cited by 28 · see citing works
Palmieri, J. R., Ratiwayanto, S., Masbar, S., Tirtokusumo, S., Rusch, J., & Marwoto, H. A. (1985) · PubMed · Cited by 27 · see citing works
High prevalence of Wuchereria bancrofti infection among Myanmar migrants in Thailand
Triteeraprapab, S., Scott, A. L., Porksakorn, C., Nuchprayoon, I., & Poovorawan, Y. (2001) · Annals of Tropical Medicine and Parasitology · doi:10.1080/00034980120072248 · Cited by 26 · see citing works
Thanchomnang, T., Intapan, P. M., Lulitanond, V., Sangmaneedet, S., Chungpivat, S., Taweethavonsawat, P., Choochote, W., & Maleewong, W. (2010) · Veterinary Parasitology · doi:10.1016/j.vetpar.2009.12.002 · Cited by 26 · see citing works
Histochemical differentiation ofBrugia, Wuchereria, DirofilariaandBreinliamicrofilariae
Yen, P. K. F., & Mak, J. W. (1978) · Annals of Tropical Medicine and Parasitology · doi:10.1080/00034983.1978.11719298 · Cited by 25 · see citing works
Differentiation of Brugia malayi and Brugia pahangi by PCR-RFLP of ITS1 and ITS2.
Nuchprayoon, S., Sangprakarn, S., Junpee, A., Nithiuthai, S., Chungpivat, S., & Poovorawan, Y. (2003) · PubMed · Cited by 22 · see citing works
Thanchomnang, T., Intapan, P. M., Lulitanond, V., Choochote, W., Manjai, A., Prasongdee, T. K., & Maleewong, W. (2008) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.2008.78.509 · Cited by 21 · see citing works
Global programme to eliminate lymphatic filariasis.
(2007) · PubMed · Cited by 21 · see citing works
Lulitanond, V., Intapan, P. M., Pipitgool, V., Choochote, W., & Maleewong, W. (2004) · Parasitology Research · doi:10.1007/s00436-004-1221-6 · Cited by 18 · see citing works
Thanchomnang, T., Intapan, P. M., Chungpivat, S., Lulitanond, V., & Maleewong, W. (2010) · Parasitology Research · doi:10.1007/s00436-009-1706-4 · Cited by 16 · see citing works
Areekit, S., Kanjanavas, P., Pakpitchareon, A., Khawsak, P., Khuchareontaworn, S., Sriyaphai, T., & Chansiri, K. (2009) · PubMed · Cited by 16 · see citing works
Choochote, W., Keha, P., Sukhavat, K., Khamboonruang, C., & Sukontason, K. L. (1987) · PubMed · Cited by 14 · see citing works
Choochote, W., Leemingsawat, S., Sucharit, S., Kunnaphuti, J., Wongkamchai, S., Sukontason, K. L., & Jitpakdi, A. (2001) · PubMed · Cited by 7 · see citing works
Maleewong, W., Choochote, W., Sukhavat, K., Khamboonruang, C., & Arunyanart, C. (1987) · PubMed · Cited by 7 · see citing works
Flagged first, then by citations
Research works printed but not linked: 3
- [4]Center for Disease Control and Prevention Recommendation of International Task Force for Disease Eradication MMWR Recomm Rep 1993 42 1 38 8145708
- [7]Lim KC Mak JW Human behaviour and zoonotic disease in Malaysia Cross JH Croll N Human Ecology and Infectious Disease London, UK Academic Press 1983 49 72
- [23]Yen PK Mak JW Histochemical differentiation of Brugia, Wuchereria, Dirofilaria and Breinlia microfilariae Ann Trop Med Parasitol 1978 72 157 162 666387
Cited by
Sorted by citations
The use of molecular xenomonitoring for surveillance of mosquito-borne diseases
Cameron, M., & Ramesh, A. (2020) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2019.0816 · Cited by 40 · see citing works
Canine and feline vector-borne diseases of zoonotic concern in Southeast Asia
Nguyen, V., Dantas‐Torres, F., & Otranto, D. (2020) · Current Research in Parasitology and Vector-Borne Diseases · doi:10.1016/j.crpvbd.2020.100001 · Cited by 36 · see citing works
Simo, G., Suh, P. F., Farikou, O., Ndjeuto-Tchouli, P. I. N., Tchouomene-Labou, J., Njiokou, F., & Asonganyi, T. (2015) · Parasites & Vectors · doi:10.1186/s13071-015-1156-z · Cited by 35 · see citing works
Kamyingkird, K., Junsiri, W., Chimnoi, W., Kengradomkij, C., Saengow, S., Sangchuto, K., Kajeerum, W., Pangjai, D., Nimsuphan, B., Inpankeaw, T., et al. (2017) · Agriculture and Natural Resources · doi:10.1016/j.anres.2017.05.003 · Cited by 28 · see citing works
Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases
Rogers, M. J., McManus, D. P., Muhi, S., & Gordon, C. A. (2021) · Clinical Microbiology Reviews · doi:10.1128/cmr.00329-20 · Cited by 23 · see citing works
Bhuvaneswari, A., Shriram, A. N., Raju, H. K., & Kumar, A. (2023) · Pathogens · doi:10.3390/pathogens12121406 · Cited by 22 · see citing works
Masny, A., Sałamatin, R., Rozej-Bielicka, W., & Golab, E. (2016) · Parasitology Research · doi:10.1007/s00436-015-4767-6 · Cited by 20 · see citing works
The Incidence of Dirofilaria immitis in Shelter Dogs and Mosquitoes in Austria
Sonnberger, K., Fuehrer, H.-P., Sonnberger, B. W., & Leschnik, M. (2021) · Pathogens · doi:10.3390/pathogens10050550 · Cited by 19 · see citing works
Jongthawin, J., Intapan, P. M., Lulitanond, V., Sanpool, O., Thanchomnang, T., Sadaow, L., & Maleewong, W. (2016) · Parasitology Research · doi:10.1007/s00436-016-5051-0 · Cited by 19 · see citing works
Detecting Dirofilaria immitis: Current Practices and Novel Diagnostic Methods
Pietrzak, D., Łuczak, J. W., & Wiśniewski, M. (2024) · Pathogens · doi:10.3390/pathogens13110950 · Cited by 19 · see citing works
Arbabi, M., Hooshyar, H., Lotfinia, M., & Bakhshi, M. A. (2020) · Molecular and Biochemical Parasitology · doi:10.1016/j.molbiopara.2020.111260 · Cited by 14 · see citing works
Beyond Tradition: Exploring Cutting-Edge Approaches for Accurate Diagnosis of Human Filariasis
Pietrzak, D., Łuczak, J. W., & Wiśniewski, M. (2024) · Pathogens · doi:10.3390/pathogens13060447 · Cited by 10 · see citing works
Sanpool, O., Tantrawatpan, C., Thanchomnang, T., Janwan, P., Intapan, P. M., Rodpai, R., Lulitanond, V., Taweethavonsawat, P., & Maleewong, W. (2016) · Vector-Borne and Zoonotic Diseases · doi:10.1089/vbz.2015.1914 · Cited by 5 · see citing works
High resolution melting real-time PCR detect and identify filarial parasites in domestic cats
Lek‐Uthai, U., Nonsaithong, D., Yotmek, S., Yotmek, S., Nochote, H., Wongkamchai, S., & Roytrakul, S. (2018) · Asian Pacific Journal of Tropical Medicine · doi:10.4103/1995-7645.248340 · Cited by 4 · see citing works
Holderman, C. J., Abruzzo, N. O., Abdelsamad, N., Kaufman, P. E., & DiGennaro, P. M. (2020) · Journal of Medical Entomology · doi:10.1093/jme/tjaa272 · Cited by 3 · see citing works
Microfilaria Positification Test Using Real-Time PCR Technique with HRM (High-Resolution Melting)
Ihsan, B. M., Berbudi, A., Agoes, R., & Hanif, M. I. (2021) · Acta Parasitologica · doi:10.1007/s11686-021-00412-5 · Cited by 2 · see citing works
Ihsan, B. M., Sucipto, C. D., & Khayan, K. (2021) · Journal Of Vocational Health Studies · doi:10.20473/jvhs.v5.i1.2021.26-30 · Cited by 1 · see citing works
КСЕНОМОНІТОРИНГОВІ ДОСЛІДЖЕННЯ КОМАРІВ ЗА ДИРОФІЛЯРІОЗУ
Poliukhovych, V. (2024) · Bulletin of Sumy National Agrarian University The series Veterinary Medicine · doi:10.32782/bsnau.vet.2024.1.8 · Cited by 1 · see citing works
IDENTIFICATION OF MICROFILARIAE USING CONVENTIONAL POLYMERASE CHAIN REACTION AND QPCR-HRM
Ihsan, B. M., Puspita, W. L., Triana, L., Wahdaniah, W., Khayan, & Sucipto, C. D. (2024) · Journal Of Vocational Health Studies · doi:10.20473/jvhs.v8.i1.2024.42-47
Ravishankar, V., Chinnadurai, R. K., Mohanty, A. K., Narayanan, S., & Shandil, R. K. (2026) · Frontiers in Tropical Diseases · doi:10.3389/fitd.2026.1803891
- Authors
- Thanchomnang, T., Intapan, P. M., Tantrawatpan, C., Lulitanond, V., Chungpivat, S., Taweethavonsawat, P., Kaewkong, W., Sanpool, O., Janwan, P., Choochote, W., & Maleewong, W.
- Affiliations
- Mahasarakham University (TH)
- Khon Kaen University (TH)
- Thammasat University (TH)
- Chulalongkorn University (TH)
- Chiang Mai University (TH)
- Corresponding author
- Maleewong, Wanchai
- ORCID
- Venue
- The Korean Journal of Parasitology
- Publisher
- Korean Society for Parasitology
- Published
- 2013-12-31
- Volume
- 51
- Issue
- 6
- Pages
- 645-650
- ISSN
- 0023-4001 (ISSN-L)
- 1738-0006
- PMC
- PMC3916452
- OpenAlex
- W2066648959
- Cited by
- 20
- References
- 28
- Citations by year
- 2026: 1 · 2024: 4 · 2023: 1 · 2021: 4 · 2020: 4 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 2
- FWCI
- 0.97
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- cc-by-nc
- Locations
- Korean Journal of Parasitology (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Funders
- Higher Education Research Promotion
- Khon Kaen University (RTA5580004)
- National Science and Technology Development Agency
- Thailand Research Fund (RTA5580004)
- Office of the Higher Education Commission
- Topics
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Keywords
- Wuchereria bancrofti, Brugia malayi, Brugia pahangi, Dirofilaria immitis, filarial nematodes, mosquito vectors, high-resolution melting analysis, 5S rRNA, xenomonitoring, molecular identification
- MeSH terms
- Animals, Blood, Brugia, Cats, Dirofilaria immitis, Dogs, Humans, Male, Culicidae, Parasitology, RNA, Ribosomal, 5S, Sensitivity and Specificity, Wuchereria bancrofti, RNA, Helminth, Transition Temperature, Real-Time Polymerase Chain Reaction
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
