A multiplex PCR for the simultaneous detection of species of filarioids infesting dogs
Abstract
The present study reports the applicability of a multiplex PCR for the simultaneous detection and differentiation of common filarioids infecting dogs, i.e., Dirofilaria immitis, Dirofilaria repens, Acanthocheilonema reconditum and Cercopithifilaria sp. Amplicons of different sizes (i.e., 170 bp, 480 bp, 590 bp and 300 bp, respectively) of regions within the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene were amplified on a single-step multiplex PCR using a mix of species-specific forward primers coupled with a single reverse primer. Experiments were carried out by amplifying genomic DNA extracted from blood or skin samples test-positive for microfilariae (mff). The number of mff present in each blood sample was quantified (from 800 to 25,000 mff/ml for A. reconditum and D. repens, respectively) and mixed blood samples were tested for the simultaneous detection of DNA from these mff. Specific amplicons for blood-circulating mff of A. reconditum, D. immitis and D. repens and for those whose adults are localized in skin (i.e., A. reconditum and Cercopithifilaria sp.) were simultaneously detected on agarose gel up to a dilution of 250 mff/ml for D. repens. The specific identity of the amplicons was confirmed by sequencing. The multiplex PCR assay reported herein represents a new tool for the molecular detection and differentiation of canine filarioids in blood and skin samples.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
A multiplex PCR based on amplification of a mitochondrial gene of blood-circulating microfilariae of D. immitis, A. reconditum and D. repens and of Cercopithifilaria spp. is useful for their molecular detection and differentiation in blood and skin samples.
“A multiplex PCR based on the amplification of a mitochondrial gene of blood-circulating microfilariae of D. immitis, A. reconditum and D. repens and of Cercopithifilaria spp. has also been shown to be useful for their molecular detection and differentiation in blood and skin samples [131].”
From Vector-borne helminths of dogs and humans in Europe (2013) · cites this paper as [131]
Screening for Dirofilaria spp. using PCR showed a sensitivity and specificity of 100% in various studies.
“Screening for Dirofilaria spp. using PCR showed a sensitivity and specificity of 100% in various studies [43,44,45].”
From The Incidence of Dirofilaria immitis in Shelter Dogs and Mosquitoes in Austria (2021) · cites this paper as [45]
Cites
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
Altschul, S. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.17.3389 · Cited by 73,302 · see citing works
Hebert, P. D. N., Ratnasingham, S., & de Waard, J. R. (2003) · Proceedings of the Royal Society of London. Series B: Biological Sciences · doi:10.1098/rsbl.2003.0025 · Cited by 4,466 · see citing works
Helminths, Arthropods and Protozoa of Domesticated Animals
Soulsby (1983) · Cited by 2,134 · see citing works
Helminths, Arthropods and Protozoa of Domesticated Animals
Beaver, P. C. (1983) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.1983.32.906 · Cited by 1,448 · see citing works
Chapter 4 Heartworm Disease in Animals and Humans
McCall, J. W., Genchi, C., Kramer, L. H., Guerrero, J., & Venco, L. (2008) · Advances in Parasitology · doi:10.1016/s0065-308x(08)00204-2 · Cited by 609 · see citing works
Otranto, D., Brianti, E., Latrofa, M. S., Annoscia, G., Weigl, S., Lia, R. P., Gaglio, G., Napoli, E., Giannetto, S., Papadopoulos, E., et al. (2012) · Parasites & Vectors · doi:10.1186/1756-3305-5-1 · Cited by 456 · see citing works
Self-citationCanine and feline cardiopulmonary parasitic nematodes in Europe: emerging and underestimated
Traversa, D., Di Cesare, A., & Conboy, G. (2010) · Parasites & Vectors · doi:10.1186/1756-3305-3-62 · Cited by 351 · see citing works
Self-citationFerri, E., Barbuto, M., Bain, O., Galimberti, A., Uni, S., Guerrero, R., Ferté, H., Bandi, C., Martin, C., & Casiraghi, M. (2009) · Frontiers in Zoology · doi:10.1186/1742-9994-6-1 · Cited by 324 · see citing works
Discrimination between six species of canine microfilariae by a single polymerase chain reaction
Rishniw, M., Barr, S. C., Simpson, K. W., Frongillo, M. F., Franz, M., & Dominguez Alpizar, J. L. (2006) · Veterinary Parasitology · doi:10.1016/j.vetpar.2005.10.013 · Cited by 307 · see citing works
Casiraghi, M., Bain, O., Guerrero, R., Martin, C., Pocacqua, V., Gardner, S. L., Franceschi, A., & Bandi, C. (2003) · International Journal for Parasitology · doi:10.1016/j.ijpara.2003.10.004 · Cited by 304 · see citing works
Pampiglione, S., & Rivasi, F. (2000) · PubMed · Cited by 264 · see citing works
Orihel, T. C., & Eberhard, M. L. (1998) · Clinical Microbiology Reviews · doi:10.1128/cmr.11.2.366 · Cited by 247 · see citing works
PCR technology, current innovations
Tracy, S., & Romero, J. (1995) · Virus Research · doi:10.1016/0168-1702(95)90075-6 · Cited by 242 · see citing works
Human dirofilariasis due to Dirofilaria (Nochtiella) repens: a review of world literature.
Pampiglione, S., Trotti, G. C., & Rivasi, F. (1995) · PubMed · Cited by 218 · see citing works
Dirofilarial Infections in Europe
Genchi, C., Kramer, L. H., & Rivasi, F. (2011) · Vector-Borne and Zoonotic Diseases · doi:10.1089/vbz.2010.0247 · Cited by 214 · see citing works
Aedes albopictus is a natural vector of Dirofilaria immitis in Italy
Cancrini, G., Frangipane di Regalbono, A., Ricci, I., Tessarin, C., Gabrielli, S., & Pietrobelli, M. (2003) · Veterinary Parasitology · doi:10.1016/j.vetpar.2003.10.011 · Cited by 206 · see citing works
Zoonotic helminths affecting the human eye
Otranto, D., & Eberhard, M. L. (2011) · Parasites & Vectors · doi:10.1186/1756-3305-4-41 · Cited by 203 · see citing works
Self-citationCanine and feline vector-borne diseases in Italy: current situation and perspectives
Otranto, D., & Dantas-Torres, F. (2010) · Parasites & Vectors · doi:10.1186/1756-3305-3-2 · Cited by 201 · cited 1× · see citing works
“Blood samples negative by the Knott’s test and skin samples btained from 10, 120 days old purpose-bred beagles in a previous tudy (Otranto et al., 2010, 2011) were included as controls.”
MethodSelf-citationWhat is new about animal and human dirofilariosis?
Simón, F., Morchón, R., González-Miguel, J., Marcos-Atxutegi, C., & Siles-Lucas, M. (2009) · Trends in Parasitology · doi:10.1016/j.pt.2009.06.003 · Cited by 135 · see citing works
Diagnosis of Canine Vector-Borne Diseases in Young Dogs: a Longitudinal Study
Otranto, D., Testini, G., Dantas‐Torres, F., Latrofa, M. S., Diniz, P. P. V. D. P., de Caprariis, D., Lia, R. P., Mencke, N., Stanneck, D., Capelli, G., et al. (2010) · Journal of Clinical Microbiology · doi:10.1128/jcm.00379-10 · Cited by 120 · see citing works
Self-citationEsch, G. W., Orihel, T. C., & Ash, L. R. (1995) · Journal of Parasitology · doi:10.2307/3283864 · Cited by 107 · see citing works
Nuchprayoon, S., Junpee, A., Poovorawan, Y., & Scott, A. L. (2005) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.2005.73.895 · Cited by 104 · see citing works
Pampiglione, S., Rivasi, F., & Gustinelli, A. (2009) · Histopathology · doi:10.1111/j.1365-2559.2008.03197_a.x · Cited by 96 · see citing works
A simple molecular method for discriminating common filarial nematodes of dogs (Canis familiaris)
Casiraghi, M., Bazzocchi, C., Mortarino, M., Ottina, E., & Genchi, C. (2006) · Veterinary Parasitology · doi:10.1016/j.vetpar.2006.06.006 · Cited by 96 · see citing works
Polymerase chain reaction—identification of <i>Dirofilaria repens</i> and <i>Dirofilaria immitis</i>
Favia, G., Lanfrancotti, A., Torre, A. D., Cancrini, G., & Coluzzi, M. (1996) · Parasitology · doi:10.1017/s0031182000067615 · Cited by 88 · see citing works
Gioia, G., Lecová, L., Genchi, M., Ferri, E., Genchi, C., & Mortarino, M. (2010) · Veterinary Parasitology · doi:10.1016/j.vetpar.2010.04.027 · Cited by 82 · see citing works
Otranto, D., Brianti, E., Dantas‐Torres, F., Weigl, S., Latrofa, M. S., Gaglio, G., Cauquil, L., Giannetto, S., & Bain, O. (2011) · Veterinary Parasitology · doi:10.1016/j.vetpar.2011.05.043 · Cited by 80 · see citing works
Self-citationHistochemical Differentiation of Dirofilaria immitis and Dipetalonema reconditum
Chalifoux, L. V., & Hunt, R. D. (1971) · Journal of the American Veterinary Medical Association · doi:10.2460/javma.1971.158.05.601 · Cited by 65 · see citing works
Mar, P.-H., Yang, I.-C., Chang, G.-N., & Fei, A. C.-Y. (2002) · Veterinary Parasitology · doi:10.1016/s0304-4017(02)00032-8 · Cited by 64 · see citing works
Brianti, E., Otranto, D., Dantas-Torres, F., Weigl, S., Latrofa, M. S., Gaglio, G., Napoli, E., Brucato, G., Cauquil, L., Giannetto, S., et al. (2012) · Veterinary Parasitology · doi:10.1016/j.vetpar.2011.07.031 · Cited by 64 · see citing works
Self-citationDipetalonema reconditum in the human eye
HUYNH, T. (2001) · British Journal of Ophthalmology · doi:10.1136/bjo.85.11.1384i · Cited by 60 · see citing works
Peribáñez, M. A., Lucientes, J., Arce, S., Morales, M., Castillo, J. A., & Gracia, M. J. (2001) · Veterinary Parasitology · doi:10.1016/s0304-4017(01)00516-7 · Cited by 43 · see citing works
[Detection of Dirofilaria in Austrian dogs].
Duscher, G. G., Feiler, A., Wille-Piazzai, W., Bakonyi, T., Leschnik, M. W., Miterpáková, M., Kolodziejek, J., Nowotny, N. R., & Joachim, A. (2009) · PubMed · Cited by 38 · see citing works
Lee, S.-E., Kim, H.-C., Chong, S.-T., Klein, T. A., & Lee, W.-J. (2007) · Veterinary Parasitology · doi:10.1016/j.vetpar.2007.04.015 · Cited by 35 · see citing works
Mishra, K., Raj, D. K., Hazra, R. K., Dash, A. P., & Supakar, P. C. (2007) · Molecular and Cellular Probes · doi:10.1016/j.mcp.2007.05.001 · Cited by 31 · see citing works
Goodey, T. (1926) · Journal of Helminthology · doi:10.1017/s0022149x00002091 · Cited by 29 · see citing works
Favia, G., Lanfrancotti, A., della Torre, A., Cancrini, G., & Coluzzi, M. (1997) · PubMed · Cited by 29 · see citing works
Chandrasekharan, N. V., Karunanayake, E. H., Franzen, L., Abeyewickreme, W., & Pettersson, U. (1994) · Experimental Parasitology · doi:10.1006/expr.1994.1029 · Cited by 28 · see citing works
Canine filarial infections in Tuscany, central Italy
Magi, M., Guardone, L., Prati, M. C., Tozzini, G., Torracca, B., Monni, G., & Macchioni, F. (2012) · Journal of Helminthology · doi:10.1017/s0022149x11000113 · Cited by 27 · see citing works
Cox, F. E. G. (1995) · Parasitology Today · doi:10.1016/0169-4758(95)80034-4 · Cited by 26 · see citing works
Thanchomnang, T., Intapan, P. M., Lulitanond, V., Sangmaneedet, S., Chungpivat, S., Taweethavonsawat, P., Choochote, W., & Maleewong, W. (2010) · Veterinary Parasitology · doi:10.1016/j.vetpar.2009.12.002 · Cited by 26 · see citing works
Differentiation of Brugia malayi and Brugia pahangi by PCR-RFLP of ITS1 and ITS2.
Nuchprayoon, S., Sangprakarn, S., Junpee, A., Nithiuthai, S., Chungpivat, S., & Poovorawan, Y. (2003) · PubMed · Cited by 22 · see citing works
Flagged first, then by citations
2 of the 44 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Human and animal dirofilariasis: The emergence of a zoonotic mosaic
Simón, F., Siles-Lucas, M., Morchón, R., González-Miguel, J., Mellado, I., Carretón, E., & Montoya-Alonso, J. A. (2012) · Clinical Microbiology Reviews · doi:10.1128/cmr.00012-12 · Cited by 834 · see citing works
Vector-borne helminths of dogs and humans in Europe
Otranto, D., Dantas-Torres, F., Brianti, E., Traversa, D., Petrić, D., Genchi, C., & Capelli, G. (2013) · Parasites & Vectors · doi:10.1186/1756-3305-6-16 · Cited by 336 · see citing works
Self-citationRecent advances on Dirofilaria repens in dogs and humans in Europe
Capelli, G., Genchi, C., Baneth, G., Bourdeau, P., Brianti, E., Cardoso, L., Danesi, P., Fuehrer, H.-P., Giannelli, A., Ionică, A. M., et al. (2018) · Parasites & Vectors · doi:10.1186/s13071-018-3205-x · Cited by 298 · see citing works
Self-citationMagnis, J., Lorentz, S., Guardone, L., Grimm, F., Magi, M., Naucke, T. J., & Deplazes, P. (2013) · Parasites & Vectors · doi:10.1186/1756-3305-6-48 · Cited by 169 · see citing works
Overview on Dirofilaria immitis in the Americas, with notes on other filarial worms infecting dogs
Dantas‐Torres, F., & Otranto, D. (2020) · Veterinary Parasitology · doi:10.1016/j.vetpar.2020.109113 · Cited by 83 · see citing works
Self-citationIonică, A. M., Matei, I. A., Mircean, V., Dumitrache, M. O., D’Amico, G., Győrke, A., Pantchev, N., Annoscia, G., Albrechtová, K., Otranto, D., et al. (2015) · Parasitology Research · doi:10.1007/s00436-014-4263-4 · Cited by 70 · see citing works
Self-citationFilarioid infections in wild carnivores: a multispecies survey in Romania
Ionică, A. M., Matei, I. A., D’Amico, G., Ababii, J., Daskalaki, A. A., Sándor, A. D., Enache, D. V., Gherman, C. M., & Mihalca, A. D. (2017) · Parasites & Vectors · doi:10.1186/s13071-017-2269-3 · Cited by 66 · see citing works
Nodular lesions due to infestation by D irofilaria repens in dogs from I taly
Albanese, F., Abramo, F., Braglia, C., Caporali, C., Venco, L., Vercelli, A., Ghibaudo, G., Leone, F., Carrani, F., Giannelli, A., et al. (2013) · Veterinary Dermatology · doi:10.1111/vde.12009 · Cited by 64 · see citing works
Self-citationMircean, M. V., Ionică, A. M., Mircean, V., Györke, A., Codea, A. R., Tăbăran, F., Taulescu, M. A., & Dumitrache, M. O. (2017) · Parasitology International · doi:10.1016/j.parint.2017.02.003 · Cited by 56 · see citing works
Liotta, J. L., Sandhu, G., Rishniw, M., & Bowman, D. D. (2013) · Journal of Parasitology · doi:10.1645/12-10.1 · Cited by 54 · see citing works
Filarioids infecting dogs in northeastern Brazil
Ramos, R. A. N., do Rêgo, A. G. D. O., Firmino, E. D. D. F., Ramos, C. A. D. N., de Carvalho, G. A., Dantas‐Torres, F., Otranto, D., & Alves, L. C. (2016) · Veterinary Parasitology · doi:10.1016/j.vetpar.2016.06.025 · Cited by 46 · see citing works
Self-citationDirofilaria immitis and D. repens show circadian co-periodicity in naturally co-infected dogs
Ionică, A. M., Matei, I. A., D’Amico, G., Bel, L. V., Dumitrache, M. O., Modrý, D., & Mihalca, A. D. (2017) · Parasites & Vectors · doi:10.1186/s13071-017-2055-2 · Cited by 43 · see citing works
Azhahianambi, P., Gopi, J., Gr, B., Aravind, M., Narendran, R. R., Latha, B. R., & Raman, M. (2018) · Acta Tropica · doi:10.1016/j.actatropica.2018.08.028 · Cited by 43 · see citing works
Role of golden jackals (Canis aureus) as natural reservoirs of Dirofilaria spp. in Romania
Ionică, A. M., Matei, I. A., D’Amico, G., Daskalaki, A. A., Juránková, J., Ionescu, D. T., Mihalca, A. D., Modrý, D., & Gherman, C. M. (2016) · Parasites & Vectors · doi:10.1186/s13071-016-1524-3 · Cited by 41 · see citing works
Human Pulmonary Dirofilariasis: A Review for the Clinicians
Saha, B. K., Bonnier, A., Chong, W. H., Chieng, H., Austin, A., Hu, K., & Shkolnik, B. (2021) · The American Journal of the Medical Sciences · doi:10.1016/j.amjms.2021.07.017 · Cited by 42 · see citing works
Otranto, D., Varcasia, A., Solinas, C., Scala, A., Brianti, E., Dantas‐Torres, F., Annoscia, G., Martin, C., Mutafchiev, Y., & Bain, O. (2013) · Parasites & Vectors · doi:10.1186/1756-3305-6-132 · Cited by 41 · see citing works
Self-citationDiagnostic challenges and the unwritten stories of dog and cat parasites
Otranto, D. (2015) · Veterinary Parasitology · doi:10.1016/j.vetpar.2015.06.002 · Cited by 40 · see citing works
Self-citationDirofilaria repens: emergence of autochthonous human infections in the Czech Republic (case reports)
Matějů, J., Chanová, M., Modrý, D., Mitková, B., Hrazdilová, K., Žampachová, V., & Kolářová, L. (2016) · BMC Infectious Diseases · doi:10.1186/s12879-016-1505-3 · Cited by 40 · see citing works
Molecular characterization of Dirofilaria spp. circulating in Portugal
Ferreira, C., Afonso, A., Calado, M., Maurício, I., Alho, A. M., Meireles, J., Madeira de Carvalho, L., & Belo, S. (2017) · Parasites & Vectors · doi:10.1186/s13071-017-2180-y · Cited by 33 · see citing works
Napoli, E., Brianti, E., Falsone, L., Gaglio, G., Foit, S., Abramo, F., Annoscia, G., Dantas‐Torres, F., Giannetto, S., & Otranto, D. (2014) · Parasitology · doi:10.1017/s0031182014001000 · Cited by 33 · see citing works
Self-citationMicrofilaruria by Dirofilaria immitis in a dog: a rare clinical pathological finding
Monobe, M. M. D. S., da Silva, R. C., Araújo, J. P., & Takahira, R. K. (2017) · Journal of Parasitic Diseases · doi:10.1007/s12639-017-0892-8 · Cited by 32 · see citing works
Potters, I., Vanfraechem, G., & Bottieau, E. (2018) · Emerging infectious diseases · doi:10.3201/eid2409.180462 · Cited by 29 · see citing works
Kaur, N., Singh, H., Sharma, P., Singh, N. K., Kashyap, N., & Singh, N. (2020) · Acta Tropica · doi:10.1016/j.actatropica.2020.105713 · Cited by 25 · see citing works
The detection of different Dirofilaria species using direct PCR technique
Silbermayr, K., Eigner, B., Duscher, G. G., Joachim, A., & Fuehrer, H. (2013) · Parasitology Research · doi:10.1007/s00436-013-3682-y · Cited by 24 · see citing works
Ciucă, L., Vismarra, A., Lebon, W., Beugnet, F., Morchón, R., Rinaldi, L., Cringoli, G., Kramer, L. H., & Genchi, M. (2020) · Veterinary Parasitology · doi:10.1016/j.vpoa.2020.100029 · Cited by 21 · see citing works
Thanchomnang, T., Intapan, P. M., Tantrawatpan, C., Lulitanond, V., Chungpivat, S., Taweethavonsawat, P., Kaewkong, W., Sanpool, O., Janwan, P., Choochote, W., et al. (2013) · The Korean Journal of Parasitology · doi:10.3347/kjp.2013.51.6.645 · Cited by 20 · see citing works
Şimşek, S., & Çiftçi, A. T. (2016) · PubMed Central · Cited by 20 · see citing works
The Incidence of Dirofilaria immitis in Shelter Dogs and Mosquitoes in Austria
Sonnberger, K., Fuehrer, H.-P., Sonnberger, B. W., & Leschnik, M. (2021) · Pathogens · doi:10.3390/pathogens10050550 · Cited by 19 · see citing works
Bamorovat, M., Sharifi, I., Harandi, M. F., Nasibi, S., Sadeghi, B., Khedri, J., & Mohammadi, M. A. (2017) · PubMed Central · Cited by 19 · see citing works
Heartworm adulticide treatment: a tropical perspective
Dantas‐Torres, F., Ketzis, J. K., Tort, G. P., Mihalca, A. D., Baneth, G., Otranto, D., Watanabe, M., Linh, B. K., Inpankaew, T., Borrás, P., et al. (2023) · Parasites & Vectors · doi:10.1186/s13071-023-05690-8 · Cited by 20 · see citing works
Self-citationFilarial infections in domestic dogs in Lusaka, Zambia
Siwila, J., Mwase, E. T., Nejsum, P. L., & Simonsen, P. E. (2015) · Veterinary Parasitology · doi:10.1016/j.vetpar.2015.04.009 · Cited by 15 · see citing works
Massive microfilaremia in a dog subclinically infected with Acanthocheilonema dracunculoides
Muñoz-Hernández, C., Gonzálvez, M., Rojas, A., Martínez‐Carrasco, C., Baneth, G., Berriatua, E., & Ortíz, J. M. (2020) · Parasitology International · doi:10.1016/j.parint.2020.102070 · Cited by 16 · see citing works
Cercopithifilaria spp. in ticks of companion animals from Asia: new putative hosts and vectors
Bezerra‐Santos, M. A., de Macedo, L. O., Nguyen, V., Manoj, R. R. S., Laidoudi, Y., Latrofa, M. S., Beugnet, F., & Otranto, D. (2022) · Ticks and Tick-borne Diseases · doi:10.1016/j.ttbdis.2022.101957 · Cited by 15 · see citing works
Self-citationFerrari, P. A., Grisolia, A., Reale, S., Liotta, R., Mularoni, A., & Bertani, A. (2018) · Journal of Cardiothoracic Surgery · doi:10.1186/s13019-018-0750-5 · Cited by 14 · see citing works
Espinosa, N., Rosero, A., Villegas, C. L., Garcia, I. C., Gaviria-Cantin, T., Nieto, A. P., Ferro, B. E., & Ramírez, L. M. N. (2022) · Pathogens · doi:10.3390/pathogens11121434 · Cited by 14 · see citing works
Vieira, V. M. D. A., Moura-Martiniano, N. O., da Silva, P. P., Paulino, É. T., Fernandes, P. D. A., Labarthe, N., Gazêta, G. S., & Neto, A. H. A. D. M. (2022) · Parasitology Research · doi:10.1007/s00436-022-07433-7 · Cited by 12 · see citing works
Canine vector-borne parasites in the Galapagos
Culda, C. A., Rodriguez, N. G. T., Puleo, F., Polit, R., Panait, L. C., Cazan, C. D., Deak, G., León, R., Vinueza, R. L., Páez‐Rosas, D., et al. (2024) · Parasites & Vectors · doi:10.1186/s13071-024-06592-z · Cited by 11 · see citing works
Solgi, R., Sadjjadi, S. M., Mohebali, M., Zarei, Z., Golkar, M., & Raz, A. (2018) · Iranian Biomedical Journal · doi:10.29252/ibj.22.4.283 · Cited by 11 · see citing works
Systemic Infection With Dirofilaria repens in Southwestern France
Blaizot, R., Receveur, M., Millet, P. G., Otranto, D., & Malvy, D. J.-M. (2017) · Annals of Internal Medicine · doi:10.7326/l17-0426 · Cited by 10 · see citing works
Self-citationNapoli, E., Bono, V., Gaglio, G., Giannetto, S., Zanghì, A., Otranto, D., & Brianti, E. (2019) · Comparative Immunology Microbiology and Infectious Diseases · doi:10.1016/j.cimid.2019.06.007 · Cited by 10 · see citing works
Self-citationDermal microfilariae of dogs, jackals and cats in different regions of Iran
Sazmand, A., Bahiraei, Z., Nemati, F., Annoscia, G., Bezerra‐Santos, M. A., Nayebzadeh, H. H., Salemi, A. M., Mousavi, S. M., Sadjjadi, S. M., & Otranto, D. (2022) · Parasites & Vectors · doi:10.1186/s13071-021-05141-2 · Cited by 9 · see citing works
Self-citationCercopithifilaria spp. of dogs: little known but prevalent filarioids beneath the skin
Bezerra‐Santos, M. A., Dantas‐Torres, F., Ramos, R. A. N., Brianti, E., & Otranto, D. (2023) · Parasites & Vectors · doi:10.1186/s13071-023-06007-5 · Cited by 8 · see citing works
Self-citationDirofilariosis in humans and animals: Two faces of one disease
Lewandowicz‐Uszyńska, A., Borawski, W., & Pasternak, G. (2019) · Postępy Higieny i Medycyny Doświadczalnej · doi:10.5604/01.3001.0013.0842 · Cited by 8 · see citing works
Raele, D. A., Pugliese, N., Bella, G. L., Calvario, A., Scarasciulli, M. L., Vasco, I., Salandra, G. L., & Cafiero, M. A. (2021) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.20-1360 · Cited by 7 · see citing works
Zanfagnini, L. G., da Silva, T. P., Campos, D. R., de Souza, S. F., Malavazi, P. F. N. D. S., de Oliveira, R. S., Daudt, C., & Pacheco, A. D. (2023) · Brazilian Journal of Veterinary Medicine/Revista Brasileira de Medicina Veterinária · doi:10.29374/2527-2179.bjvm000223 · Cited by 7 · see citing works
Canine Filariasis Outbreak in Southwestern Colombia: A Molecular and Epidemiological StudyPreprint
Espinosa, N., Rosero, A., Villegas, C. L., Garcia, I. C., Gaviria-Cantin, T., Peña, A., Ferro, B. E., & Ramírez, L. M. N. (2020) · Preprints.org · doi:10.20944/preprints202010.0221.v1 · Cited by 5 · see citing works
Veterinary Diagnosis of Filarial Infection
Evans, C. C., Pilotte, N., Williams, S. A., & Moorhead, A. R. (2022) · Wiley eBooks · doi:10.1002/9783527823413.ch6 · Cited by 6 · see citing works
Dumitrache, M. O., D’Amico, G., Voiniţchi, E., Maximenco, S., Mircean, V., & Ionică, A. M. (2021) · Parasites & Vectors · doi:10.1186/s13071-021-04891-3 · Cited by 6 · see citing works
Singh, H., Padmaja, M., Thomas, A. M., Panwar, H., Nasrul, S. I., Jyoti, J., & Singh, N. (2024) · Acta Parasitologica · doi:10.1007/s11686-024-00856-5 · Cited by 6 · see citing works
Vector-borne pathogens in dogs in Egypt: Molecular and immunological insights.
Khalifa, M. M., Salem, M. A., Fouad, E., Bakry, N., Kamel, M. S., El-Bahy, M. M., & Ramadan, R. M. (2025) · Research in Veterinary Science · doi:10.1016/j.rvsc.2025.105629 · Cited by 9 · see citing works
Pérez-Ramírez, R. D., Lugo-Vargas, R., Petano-Duque, J. M., Cruz-Méndez, J. S., & Rondón‐Barragán, I. S. (2023) · Veterinary World · doi:10.14202/vetworld.2023.94-103 · Cited by 6 · see citing works
Ripa, A., Díaz‐Caballero, J. A., González, M. J. P., Espinosa, A., Zapata, J. L. G., & Fernández-García, J. L. (2024) · Molecular Biology Reports · doi:10.1007/s11033-023-09010-2 · Cited by 6 · see citing works
Wongkamchai, S., Mayoon, B., Kanakul, N., Foongladda, S., Wanachiwanawin, D., Nochote, H., & Loymek, S. (2015) · Vector-Borne and Zoonotic Diseases · doi:10.1089/vbz.2014.1762 · Cited by 6 · see citing works
Livingston, I., Gregory, T. M., Hawkins, E. C., Cave, A., Loyola, A., Vaden, S. L., Deresienski, D., Riofrío‐Lazo, M., Lewbart, G. A., Páez‐Rosas, D., et al. (2024) · Ecology and Evolution · doi:10.1002/ece3.70596 · Cited by 5 · see citing works
Cancino‐Faure, B., González, C. R., Piñeiro-González, A., Pinochet, S., Bustos, S., Morchón, R., Cazaux, A. P., Aguilar, I. Q., Espinoza, M. S., Salgado, R. A., et al. (2024) · Frontiers in Veterinary Science · doi:10.3389/fvets.2023.1334832 · Cited by 5 · see citing works
Bahiraei, Z., Sazmand, A., Nourian, A., & Otranto, D. (2023) · نامه انجمن حشره شناسی ایران · doi:10.52547/jesi.43.1.6 · Cited by 5 · see citing works
Self-citationSanpool, O., Tantrawatpan, C., Thanchomnang, T., Janwan, P., Intapan, P. M., Rodpai, R., Lulitanond, V., Taweethavonsawat, P., & Maleewong, W. (2016) · Vector-Borne and Zoonotic Diseases · doi:10.1089/vbz.2015.1914 · Cited by 5 · see citing works
Xing, Y., Chen, Y., Feng, C., Bao, J., Li, X., & Jiang, H. (2023) · Journal of Fungi · doi:10.3390/jof9080791 · Cited by 4 · see citing works
Further data on the distribution of Dirofilaria spp. in the Czech Republic in dogs
Juránková, J., Mitková, B., Novotná, M., Hofmannová, L., Červená, B., Bowman, D. D., & Modrý, D. (2022) · Folia Parasitologica · doi:10.14411/fp.2022.007 · Cited by 4 · see citing works
Jerše, M. (2023) · Polish Journal of Pathology · doi:10.5114/pjp.2022.125821 · Cited by 4 · see citing works
Sidhoum, N. R., Boucheikhchoukh, M., Azzouzi, C., Mechouk, N., Culda, C. A., Ionică, A. M., Balmoș, O.-M., Mihalca, A. D., & Deak, G. (2024) · Research in Veterinary Science · doi:10.1016/j.rvsc.2024.105235 · Cited by 3 · see citing works
Holderman, C. J., Abruzzo, N. O., Abdelsamad, N., Kaufman, P. E., & DiGennaro, P. M. (2020) · Journal of Medical Entomology · doi:10.1093/jme/tjaa272 · Cited by 3 · see citing works
Surveys of Canine Filarioids In Nigeria: The Path Travelled and the Way Forward
Kamani, J., & González‐Miguel, J. (2021) · Nigerian Veterinary Journal · doi:10.4314/nvj.v41i3.5 · Cited by 2 · see citing works
CANINE HEARTWORM DISEASE IN A BRAZILIAN NON-ENDEMIC AREA
Monobe, M. M. D. S., da Silva, R. C., Melanchauski, M. S., & Lopes, R. S. (2016) · Arquivos de Ciências Veterinárias e Zoologia da UNIPAR · doi:10.25110/arqvet.v19i2.2016.5927 · Cited by 2 · see citing works
Deak, G., Toader, S., Soare, D. G., Ionică, A. M., Taulescu, M. A., & Mihalca, A. D. (2022) · Frontiers in Veterinary Science · doi:10.3389/fvets.2022.941303 · Cited by 2 · see citing works
Dirofilaria repens in the Spermatic Cord of a 5-Year-Old Boy: A Rare Pediatric Case in Italy
Franzò, A., Marıno, A., Celesia, B. M., Bruno, R., Catalano, P., Cacciaguerra, S., Reale, S., Cacopardo, B. S., & Nunnari, G. (2025) · Tropical Medicine and Infectious Disease · doi:10.3390/tropicalmed10070184 · Cited by 1 · see citing works
Patent Dirofilaria immitis infection in Galapagos sea lion rookeries in San Cristóbal Island
Culda, C. A., Vinueza, R. L., Riofrío‐Lazo, M., León, R., Páez‐Rosas, D., & Mihalca, A. D. (2025) · Parasitology · doi:10.1017/s0031182025100425 · Cited by 1 · see citing works
Future Advanced Research Directions against Parasitic Diseases in Dogs and Cats
Haq, A. U., Allaie, I. M., Tramboo, S. R., & Rana, T. (2024) · doi:10.1002/9781394158256.ch23 · Cited by 1 · see citing works
Dantas‐Torres, F., Brianti, E., & Otranto, D. (2016) · Springer eBooks · doi:10.1007/978-3-319-13884-8_28
Self-citationCanine Dirofilariasis in the South Caucasus and its Pathomorphology
Tsitskishvili, L., Makaradze, L. A., Kurashvili, T., Milashvili, N., Makaradze, Z., & Sanaia, E. (2023) · Socratic Lectures · doi:10.55295/psl.2023.ii8
- Authors
- Latrofa, M. S., Weigl, S., Dantas-Torres, F., Annoscia, G., Traversa, D., Brianti, E., & Otranto, D.
- Affiliations
- University of Bari Aldo Moro (IT)
- University of Teramo (IT)
- University of Messina (IT)
- Corresponding author
- Otranto, Domenico
- Venue
- Acta Tropica
- Publisher
- Elsevier BV
- Published
- 2012-04
- Volume
- 122
- Issue
- 1
- Pages
- 150-154
- ISSN
- 0001-706X (ISSN-L)
- OpenAlex
- W1982109491
- Cited by
- 70
- References
- 44
- Citations by year
- 2025: 3 · 2024: 7 · 2023: 8 · 2022: 7 · 2021: 5 · 2020: 6 · 2019: 2 · 2018: 5 · 2017: 7 · 2016: 7 · 2015: 3 · 2014: 2 · 2013: 7 · 2012: 1
- FWCI
- 2.87
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Acta Tropica (publishedVersion)
- PubMed (publishedVersion)
- UniTERAMO Research Catalog (University of Teramo) (submittedVersion)
- CINECA IRIS Institutional Research Information System (University of Bari Aldo Moro) (submittedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
- Keywords
- Dirofilaria immitis, Dirofilaria repens, Acanthocheilonema reconditum, multiplex PCR, mitochondrial cox1 gene, microfilariae, molecular detection, species differentiation, dogs, skin samples, blood samples
- MeSH terms
- Animals, Blood, Dog Diseases, Dogs, Filariasis, Filarioidea, Parasitology, Sensitivity and Specificity, Skin, Protozoan Proteins, DNA Primers, Molecular Diagnostic Techniques, Cyclooxygenase 1, Multiplex Polymerase Chain Reaction
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
