Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis
Abstract
A real-time fluorescence resonance energy transfer (FRET) PCR supplemented with melting curve analysis for the rapid molecular detection of Dirofilaria immitis in mosquito vectors and dog blood samples was developed. This real-time FRET PCR was based on the fluorescence melting curve analysis of a hybrid between an amplicon generated from the D. immitis ribosomal RNA gene sequence and specific fluorophore-labeled probes. The sensitivity, specificity, accuracy, and positive and negative predictive values of this method were all 100%. Besides being highly sensitive and specific, this PCR is fast and offers a high throughput. Therefore it is a suitable and powerful tool for the diagnosis and for epidemiological surveys of canine dirofilariasis as well as for molecular xenomonitoring of D. immitis in mosquito vectors.
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Screening for Dirofilaria spp. using PCR showed a sensitivity and specificity of 100% in various studies.
“Screening for Dirofilaria spp. using PCR showed a sensitivity and specificity of 100% in various studies [43,44,45].”
From The Incidence of Dirofilaria immitis in Shelter Dogs and Mosquitoes in Austria (2021) · cites this paper as [43]
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Contents lists available at SciVerse ScienceDirect
Latrofa, M. S., Weigl, S., Dantas‐Torres, F., Annoscia, G., Traversa, D., Brianti, E., & Otranto, D. (2012)
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- Authors
- Thanchomnang, T., Intapan, P. M., Lulitanond, V., Sangmaneedet, S., Chungpivat, S., Taweethavonsawat, P., Choochote, W., & Maleewong, W.
- Affiliations
- Khon Kaen University (TH)
- Chulalongkorn University (TH)
- Chiang Mai University (TH)
- ORCID
- Venue
- Veterinary Parasitology
- Publisher
- Elsevier BV
- Published
- 2010-03
- Volume
- 168
- Issue
- 3-4
- Pages
- 255-260
- ISSN
- 0304-4017 (ISSN-L)
- OpenAlex
- W2070687155
- Cited by
- 26
- References
- 19
- Citations by year
- 2025: 2 · 2022: 1 · 2021: 2 · 2019: 1 · 2017: 4 · 2016: 1 · 2015: 2 · 2013: 2 · 2012: 5
- FWCI
- 2.29
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Veterinary Parasitology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Khon Kaen University
- Thailand Research Fund
- Division of Chemistry
- Office of the Higher Education Commission
- Faculty of Medicine, Prince of Songkla University
- Topics
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
- Keywords
- Dirofilaria immitis, canine dirofilariasis, mosquito vectors, xenomonitoring, melting curve analysis, ribosomal DNA, molecular diagnosis, dogs, disease surveillance
- MeSH terms
- Animals, Dirofilaria immitis, Dirofilariasis, Dogs, Female, Insect Vectors, Culicidae, Sensitivity and Specificity, Reproducibility of Results, Polymerase Chain Reaction, Fluorescence Resonance Energy Transfer
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