Cited by 26Parasitic Diseases Research and TreatmentHelminth infection and controlResearch on Leishmaniasis Studies

Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis

Thanchomnang, Tongjit, Intapan, Pewpan M., Lulitanond, Viraphong, Sangmaneedet, Somboon, Chungpivat, Sudchit, Taweethavonsawat, Piyanan, Choochote, Wej, Maleewong, Wanchai·2010·Veterinary Parasitology

Abstract

A real-time fluorescence resonance energy transfer (FRET) PCR supplemented with melting curve analysis for the rapid molecular detection of Dirofilaria immitis in mosquito vectors and dog blood samples was developed. This real-time FRET PCR was based on the fluorescence melting curve analysis of a hybrid between an amplicon generated from the D. immitis ribosomal RNA gene sequence and specific fluorophore-labeled probes. The sensitivity, specificity, accuracy, and positive and negative predictive values of this method were all 100%. Besides being highly sensitive and specific, this PCR is fast and offers a high throughput. Therefore it is a suitable and powerful tool for the diagnosis and for epidemiological surveys of canine dirofilariasis as well as for molecular xenomonitoring of D. immitis in mosquito vectors.

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Crossref · 10.1016/j.vetpar.2009.12.002

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Authors
Thanchomnang, T., Intapan, P. M., Lulitanond, V., Sangmaneedet, S., Chungpivat, S., Taweethavonsawat, P., Choochote, W., & Maleewong, W.
Affiliations
  • Khon Kaen University (TH)
  • Chulalongkorn University (TH)
  • Chiang Mai University (TH)
Venue
Veterinary Parasitology
Publisher
Elsevier BV
Published
2010-03
Volume
168
Issue
3-4
Pages
255-260
ISSN
0304-4017 (ISSN-L)
OpenAlex
W2070687155
Cited by
26
References
19
Citations by year
2025: 2 · 2022: 1 · 2021: 2 · 2019: 1 · 2017: 4 · 2016: 1 · 2015: 2 · 2013: 2 · 2012: 5
FWCI
2.29
Type
journal article
Language
en
Open access
closed
Locations
  • Veterinary Parasitology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Khon Kaen University
  • Thailand Research Fund
  • Division of Chemistry
  • Office of the Higher Education Commission
  • Faculty of Medicine, Prince of Songkla University
Topics
  • Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
  • Health Sciences › Veterinary › Small Animals › Helminth infection and control
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
Keywords
Dirofilaria immitis, canine dirofilariasis, mosquito vectors, xenomonitoring, melting curve analysis, ribosomal DNA, molecular diagnosis, dogs, disease surveillance
MeSH terms
Animals, Dirofilaria immitis, Dirofilariasis, Dogs, Female, Insect Vectors, Culicidae, Sensitivity and Specificity, Reproducibility of Results, Polymerase Chain Reaction, Fluorescence Resonance Energy Transfer

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