Is molecular xenomonitoring of mosquitoes for Dirofilaria repens suitable for dirofilariosis surveillance in endemic regions?
Abstract
Dirofilaria repens is a parasite of animals and humans, transferred by mosquitoes. The assessment of the presence of D. repens-infected vertebrate hosts in the investigated area can be performed by xenomonitoring—detection of the parasite in blood-feeding arthropods. Our study aimed to evaluate PCR xenomonitoring of mosquitoes as a tool for dirofilariosis surveillance in Poland. We were also interested whether inter-study comparisons at the international level would be possible. Mosquitoes were collected in a single locality in Mazowsze province in Poland, in which between 12 and 20% of dogs were infected with D. repens and autochthonous human dirofilariosis was confirmed. All captured female mosquitoes were divided into pools; alternatively, single mosquitoes were analyzed; DNA was isolated and subjected to PCR and real-time PCR for detection of D. repens. The estimations of infection rates of mosquitoes with D. repens, based on PCR results, varied from 0 to 1.57% even between assays for detection of distinct fragments of the same marker—cytochrome oxidase subunit one gene. Polymorphisms of the DNA sequence within binding sites of the primers used in D. repens xenomonitoring assays, applied in European studies, were identified. Non-specific amplification of Setaria tundra (Nematoda: Onchocercidae) DNA occurred. Surveillance of dirofilariosis by PCR mosquito xenomonitoring is possible; however, the efficiency of the approach on territories where the prevalence of the disease among definitive hosts is lower than 12% remains unknown. Furthermore, mosquito infection rate estimations can be PCR assay dependent, which makes inter-study comparisons difficult. The results obtained in independent European xenomonitoring studies were contradictory. International collaboration would be required to establish a standardized set of assays for sensitive and specific xenomonitoring-based dirofilariosis surveillance.
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The relative prevalence of dirofilariosis detected in this study was higher than in neighbouring Austria, the Czech Republic and Poland.
“The relative prevalence of dirofilariosis detected in our study was shown to be higher than in neighbouring Austria, the Czech Republic and Poland.”
From Urbanization impact on mosquito community and the transmission potential of filarial infection in central Europe (2018) · cites this paper as [59]
Cites
Rapid and simple method for purification of nucleic acids
Boom, R., Sol, C. J. A., Salimans, M. M. M., Jansen, C. L., Dillen, P. M. E. W., & van der Noordaa, J. (1990) · Journal of Clinical Microbiology · doi:10.1128/jcm.28.3.495-503.1990 · Cited by 5,273 · see citing works
Nematode parasites of vertebrates: their development and transmission
Anderson, R. C. (2000) · CABI Publishing eBooks · doi:10.1079/9780851994215.0000 · Cited by 2,487 · see citing works
Human and animal dirofilariasis: The emergence of a zoonotic mosaic
Simón, F., Siles-Lucas, M., Morchón, R., González-Miguel, J., Mellado, I., Carretón, E., & Montoya-Alonso, J. A. (2012) · Clinical Microbiology Reviews · doi:10.1128/cmr.00012-12 · Cited by 834 · see citing works
Discrimination between six species of canine microfilariae by a single polymerase chain reaction
Rishniw, M., Barr, S. C., Simpson, K. W., Frongillo, M. F., Franz, M., & Dominguez Alpizar, J. L. (2006) · Veterinary Parasitology · doi:10.1016/j.vetpar.2005.10.013 · Cited by 307 · see citing works
Saah, A. J., & Hoover, D. R. (1997) · Annals of Internal Medicine · doi:10.7326/0003-4819-126-1-199701010-00026 · Cited by 271 · see citing works
Katholi, C. R., Toé, L., Merriweather, A., et al. (1995) · The Journal of Infectious Diseases · doi:10.1093/infdis/172.5.1414 · Cited by 197 · see citing works
Comparison of methods for estimation of individual-level prevalence based on pooled samples
Cowling, D. W., Gardner, I. A., & Johnson, W. O. (1999) · Preventive Veterinary Medicine · doi:10.1016/s0167-5877(98)00131-7 · Cited by 178 · see citing works
Boom, R., Sol, C. J. A., Beld, M., et al. (1999) · Journal of Clinical Microbiology · doi:10.1128/jcm.37.3.615-619.1999 · Cited by 149 · see citing works
Sałamatin, R., Pavlikovska, T., Sagach, O., Nikolayenko, S., Kornyushin, V. V., Kharchenko, V., Masny, A., Cielecka, D., Konieczna-Sałamatin, J., Conn, D. B., et al. (2013) · Acta Parasitologica · doi:10.2478/s11686-013-0187-x · Cited by 118 · see citing works
Self-citationKronefeld, M., Kampen, H., Sassnau, R., & Werner, D. (2014) · Parasites & Vectors · doi:10.1186/1756-3305-7-30 · Cited by 107 · see citing works
Boyle, B., Dallaire, N., & MacKay, J. J. (2009) · BMC Biotechnology · doi:10.1186/1472-6750-9-75 · Cited by 106 · see citing works
Natural vectors of dirofilariasis in rural and urban areas of the Tuscan region, central Italy
Cancrini, G., Magi, M., Gabrielli, S., Arispici, M., Tolari, F., Dell’Omodarme, M., & Prati, M. C. (2006) · Journal of Medical Entomology · doi:10.1093/jmedent/43.3.574 · Cited by 105 · see citing works
Latrofa, M. S., Montarsi, F., Ciocchetta, S., Annoscia, G., Dantas-Torres, F., Ravagnan, S., Capelli, G., & Otranto, D. (2012) · Parasites & Vectors · doi:10.1186/1756-3305-5-76 · Cited by 77 · see citing works
Morales‐Hojas, R., Cheke, R., & Post, R. J. (2006) · Journal of Helminthology · doi:10.1079/joh2006331 · Cited by 73 · see citing works
Current Surveys on the Prevalence and Distribution of Dirofilaria spp. in Dogs in Germany
Pantchev, N., Norden, N., Lorentzen, L., Rossi, M., Rossi, U., Brand, B., & Dyachenko, V. (2009) · Parasitology Research · doi:10.1007/s00436-009-1497-7 · Cited by 66 · see citing works
First molecular identification of mosquito vectors of Dirofilaria immitis in continental Portugal
Ferreira, C. A. C., de Pinho Mixão, V., Novo, M. T. L. M., Calado, M. M. P., Gonçalves, L. A. P., Belo, S. M. D., & de Almeida, A. P. G. (2015) · Parasites & Vectors · doi:10.1186/s13071-015-0760-2 · Cited by 61 · see citing works
Human dirofilariosis in Poland: the first cases of autochthonous infections with Dirofilaria repens.
Cielecka, D., Hanna, Ż.-P., Masny, A., Sałamatin, R., Wesołowska, M., & Gołąb, E. (2012) · PubMed · Cited by 60 · see citing works
Self-citationStable Transmission ofDirofilaria repensNematodes, Northern Germany
Czajka, C., Becker, N., Jöst, H., Poppert, S., Schmidt‐Chanasit, J., Krüger, A., & Tannich, E. (2014) · Emerging infectious diseases · doi:10.3201/eid2002.131003 · Cited by 52 · see citing works
Czajka, C., Becker, N., Poppert, S., Jöst, H., Schmidt-Chanasit, J., & Krüger, A. (2012) · Parasites & Vectors · doi:10.1186/1756-3305-5-14 · Cited by 51 · see citing works
Hamel, D., Silaghi, C., Zapadynska, S., Kudrin, A., & Pfister, K. (2013) · Ticks and Tick-borne Diseases · doi:10.1016/j.ttbdis.2012.08.005 · Cited by 48 · see citing works
Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia
Bocková, E., Rudolf, I., Kočišová, A., Betášová, L., Venclíková, K., Mendel, J., & Hubálek, Z. (2013) · Parasitology Research · doi:10.1007/s00436-013-3526-9 · Cited by 48 · see citing works
Latrofa, M. S., Dantas-Torres, F., Annoscia, G., Genchi, M., Traversa, D., & Otranto, D. (2012) · Veterinary Parasitology · doi:10.1016/j.vetpar.2011.10.038 · Cited by 47 · see citing works
Zoonotic Dirofilaria repens (Nematoda: Filarioidea) in Aedes vexans mosquitoes, Czech Republic
Rudolf, I., Šebesta, O., Mendel, J., Betášová, L., Bocková, E., Jedličková, P., Venclíková, K., Blažejová, H., Šikutová, S., & Hubálek, Z. (2014) · Parasitology Research · doi:10.1007/s00436-014-4191-3 · Cited by 42 · see citing works
Autochthonous Dirofilaria repens in Austria
Silbermayr, K., Eigner, B., Joachim, A., Duscher, G. G., Seidel, B., Allerberger, F., Indra, A., Hufnagl, P., & Fuehrer, H.-P. (2014) · Parasites & Vectors · doi:10.1186/1756-3305-7-226 · Cited by 42 · see citing works
Harizanov, R., Jordanova, D. P., & Bikov, I. S. (2014) · Parasitology Research · doi:10.1007/s00436-014-3802-3 · Cited by 40 · see citing works
Primer-site SNPs mask mutations
Quinlan, A. R., & Marth, G. (2007) · Nature Methods · doi:10.1038/nmeth0307-192 · Cited by 35 · see citing works
Demiaszkiewicz, A. W., Polanczyk, G., Pyziel, A. M., Kuligowska, I., & Lachowicz, J. (2009) · PubMed · Cited by 26 · see citing works
Case of human Dirofilaria repens infection manifested by cutaneous larva migrans syndrome
Antolová, D., Miterpáková, M., & Paraličová, Z. (2015) · Parasitology Research · doi:10.1007/s00436-015-4499-7 · Cited by 25 · see citing works
Erickson, S. M., Fischer, K., Weil, G. J., et al. (2009) · Parasites & Vectors · doi:10.1186/1756-3305-2-56 · Cited by 24 · see citing works
Is Dirofilaria repens Endemic in the Havelland District in Brandenburg, Germany?
Sassnau, R., Kohn, M., Demeler, J., Kohn, B., Müller, E., Krücken, J., & von Samson‐Himmelstjerna, G. (2013) · Vector-Borne and Zoonotic Diseases · doi:10.1089/vbz.2012.1293 · Cited by 23 · see citing works
Thanchomnang, T., Intapan, P. M., Tantrawatpan, C., Lulitanond, V., Chungpivat, S., Taweethavonsawat, P., Kaewkong, W., Sanpool, O., Janwan, P., Choochote, W., et al. (2013) · The Korean Journal of Parasitology · doi:10.3347/kjp.2013.51.6.645 · Cited by 20 · see citing works
Osińska, B., Demiaszkiewicz, A. W., Pyziel, A. M., & Dolka, I. (2014) · Journal of Veterinary Research · doi:10.2478/bvip-2014-0006 · Cited by 13 · see citing works
Consequences of primer binding-sites polymorphisms on genotyping practice
Martins, E. M., Vilarinho, L., Esteves, S., et al. (2011) · Open Journal of Genetics · doi:10.4236/ojgen.2011.12004 · Cited by 11 · see citing works
Masny, A., Lewin, T., Sałamatin, R., & Golab, E. (2011) · Polish Journal of Veterinary Sciences · doi:10.2478/v10181-011-0073-0 · Cited by 8 · see citing works
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Recent advances on Dirofilaria repens in dogs and humans in Europe
Capelli, G., Genchi, C., Baneth, G., Bourdeau, P., Brianti, E., Cardoso, L., Danesi, P., Fuehrer, H.-P., Giannelli, A., Ionică, A. M., et al. (2018) · Parasites & Vectors · doi:10.1186/s13071-018-3205-x · Cited by 298 · see citing works
Čabanová, V., Miterpáková, M., Valentová, D., Blažejová, H., Rudolf, I., Stloukal, E., Hurníková, Z., & Dzidová, M. (2018) · Parasites & Vectors · doi:10.1186/s13071-018-2845-1 · Cited by 37 · see citing works
Mosquitoes in the Danube Delta: searching for vectors of filarioid helminths and avian malaria
Ionică, A. M., Zittra, C., Wimmer, V., Leitner, N., Votýpka, J., Modrý, D., Mihalca, A. D., & Fuehrer, H.-P. (2017) · Parasites & Vectors · doi:10.1186/s13071-017-2264-8 · Cited by 27 · see citing works
First molecular identification of Dirofilaria spp. (Onchocercidae) in mosquitoes from Serbia
Kurucz, K., Kepner, A., Krtinic, B., Zana, B., Földes, F., Bányai, K., Oldal, M., Jakab, F., & Kemenesi, G. (2016) · Parasitology Research · doi:10.1007/s00436-016-5126-y · Cited by 24 · see citing works
Szentivanyi, T., V. González, L., Klein, Á., Soltész, Z., & Garamszegi, L. Z. (2025) · Parasites & Vectors · doi:10.1186/s13071-025-06882-0 · Cited by 10 · see citing works
- Authors
- Masny, A., Sałamatin, R., Rozej-Bielicka, W., & Golab, E.
- Affiliations
- Narodowy Instytut Zdrowia Publicznego PZH – Państwowy Instytut Badawczy (PL)
- Medical University of Warsaw (PL)
- Corresponding author
- Masny, Aleksander
- Venue
- Parasitology Research
- Published
- 2015-10-22
- Volume
- 115
- Issue
- 2
- Pages
- 511-525
- ISSN
- 0044-3255 (ISSN-L)
- 0932-0113
- 1432-1955
- OpenAlex
- W2175700116
- Cited by
- 20
- References
- 34
- Citations by year
- 2025: 1 · 2024: 4 · 2023: 1 · 2022: 2 · 2020: 1 · 2019: 1 · 2018: 5 · 2017: 2 · 2016: 2 · 2015: 1
- FWCI
- 2.59
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Parasitology Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Keywords
- Dirofilaria repens, dirofilariasis, xenomonitoring, mosquitoes, PCR detection, dog infection, disease surveillance
- MeSH terms
- Animals, Base Sequence, Electron Transport Complex IV, Dirofilariasis, Dogs, Female, Humans, Culicidae, Poland, Prevalence, Dirofilaria repens, Real-Time Polymerase Chain Reaction
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