Finding Wolbachia in Filarial larvae and Culicidae Mosquitoes in Upper Egypt Governorate
Open accessAbstract
Wolbachia is an obligatory intracellular endosymbiotic bacterium, present in over 20% of all insects altering insect reproductive capabilities and in a wide range of filarial worms which is essential for worm survival and reproduction. In Egypt, no available data were found about Wolbachia searching for it in either mosquitoes or filarial worms. Thus, we aimed to identify the possible concurrent presence of Wolbachia within different mosquitoes and filarial parasites, in Assiut Governorate, Egypt using multiplex PCR. Initially, 6 pools were detected positive for Wolbachia by single PCR. The simultaneous detection of Wolbachia and filarial parasites (Wuchereria bancrofti, Dirofilaria immitis, and Dirofilaria repens) by multiplex PCR was spotted in 5 out of 6 pools, with an overall estimated rate of infection (ERI) of 0.24%. Unexpectedly, the highest ERI (0.53%) was for Anopheles pharoensis with related Wolbachia and W. bancrofti, followed by Aedes (0.42%) and Culex (0.26%). We also observed that Wolbachia altered Culex spp. as a primary vector for W. bancrofti to be replaced by Anopheles sp. Wolbachia within filaria-infected mosquitoes in our locality gives a hope to use bacteria as a new control trend simultaneously targeting the vector and filarial parasites.
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There are only three studies that have focused on investigating simultaneous infection with native Wolbachia and Dirofilaria in mosquitoes from natural populations.
“There are only three studies that have focused on investigating simultaneous infection with native Wolbachia and Dirofilaria in mosquitoes from natural populations [22, 23, 51].”
From <i>Dirofilaria</i> and <i>Wolbachia</i> in mosquitoes (Diptera: Culicidae) in central European Russia and on the Black Sea coast (2019) · cites this paper as [23]
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- Authors
- Dyab, A. K., Galal, L. A., Mahmoud, A. E., & Mokhtar, Y.
- Affiliations
- Assiut University (EG)
- Corresponding author
- Mahmoud, Abeer E.
- Venue
- The Korean Journal of Parasitology
- Publisher
- Korean Society for Parasitology
- Published
- 2016-06-30
- Volume
- 54
- Issue
- 3
- Pages
- 265-272
- ISSN
- 0023-4001 (ISSN-L)
- 1738-0006
- PMC
- PMC4977788
- OpenAlex
- W2471292206
- Cited by
- 13
- References
- 46
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2022: 3 · 2019: 3 · 2017: 1
- FWCI
- 1.23
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- Locations
- Korean Journal of Parasitology (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Funders
- Faculty of Medicine, Assiut University, Assiut, Egypt
- Topics
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect symbiosis and bacterial influences
- Life Sciences › Immunology and Microbiology › Parasitology › Vector-borne infectious diseases
- Keywords
- Wolbachia, filarial larvae, mosquitoes, Wuchereria bancrofti, Dirofilaria immitis, Dirofilaria repens, Anopheles pharoensis, Aedes, Culex, endosymbiotic bacteria, Upper Egypt
- MeSH terms
- Animals, Dirofilaria immitis, DNA, Bacterial, Egypt, Female, Culicidae, Wuchereria bancrofti, Wolbachia, Dirofilaria repens, Multiplex Polymerase Chain Reaction
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