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Cited by 13Tissue Engineering and Regenerative Medicine3D Printing in Biomedical ResearchHydrogels: synthesis, properties, applications

Comparative analysis of porcine-uterine decellularization for bioactive-molecule preservation and DNA removal

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Fazel Anvari Yazdi, Abbas, Tahermanesh, Kobra, Ejlali, Maryam, Babaei-Ghazvini, Amin, Acharya, Bishnu, Badea, Ildiko, MacPhee, Daniel J., Chen, Xiongbiao·2024·Frontiers in Bioengineering and Biotechnology
DOI 10.3389/fbioe.2024.1418034PMC PMC11480021
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Abstract

Introduction: Decellularized uterine extracellular matrix has emerged as a pivotal focus in the realm of biomaterials, offering a promising source in uterine tissue regeneration, research on disease diagnosis and treatments, and ultimately uterine transplantation. In this study, we examined various protocols for decellularizing porcine uterine tissues, aimed to unravel the intricate dynamics of DNA removal, bioactive molecules preservation, and microstructural alterations. Methods: Porcine uterine tissues were treated with 6 different, yet rigorously selected and designed, protocols with sodium dodecyl sulfate (SDS), Triton ® X-100, peracetic acid + ethanol, and DNase I. After decellularization, we examined DNA quantification, histological staining (H&E and DAPI), glycosaminoglycans (GAG) assay, scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and Thermogravimetric Analysis (TGA). Results: A comparative analysis among all 6 protocols was conducted with the results demonstrating that all protocols achieved decellularization; while 0.1% SDS + 1% Triton ® X-100, coupled with agitation, demonstrated the highest efficiency in DNA removal. Also, it was found that DNase I played a key role in enhancing the efficiency of the decellularization process by underscoring its significance in digesting cellular contents and eliminating cell debris by 99.79% (19.63 ± 3.92 ng/mg dry weight). Conclusions: Our findings enhance the nuanced understanding of DNA removal, GAG preservation, microstructural alteration, and protein decomposition in decellularized uterine extracellular matrix, while highlighting the importance of decellularization protocols designed for intended applications. This study along with our findings represents meaningful progress for advancing the field of uterine transplantation and related tissue engineering/regenerative medicine.

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  • Natural biopolymers such as collagen, gelatin, fibrin, silk fibroin, and decellularized extracellular matrix offer high biocompatibility and bioactivity.

    “Natural biopolymers such as collagen, gelatin, fibrin, silk fibroin, and decellularized extracellular matrix (dECM) offer high biocompatibility and bioactivity [5,6].”

    From Biomaterials in Postoperative Adhesion Barriers and Uterine Tissue Engineering (2025) · cites this paper as [6]

    Section 3.1 (page unavailable) says that retaining GAGs in decellularized uterine matrix preserves bioactive signals that facilitate cell attachment and growth. The paper does not establish high biocompatibility for the materials listed, nor does it address gelatin, fibrin, or silk fibroin as materials with these properties. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

  • Collagen is the most abundant protein, making up about 20-30% of the total protein in the human body, and plays a crucial role in the structural integrity of various tissues.

    “Collagen is the most abundant protein, making up about 20–30% of the total protein in the human body, and plays a crucial role in the structural integrity of various tissues.”

    From Biomaterials in Postoperative Adhesion Barriers and Uterine Tissue Engineering (2025) · cites this paper as [6]

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Cited by

Sorted by citations

Authors
Fazel Anvari Yazdi, A., Tahermanesh, K., Ejlali, M., Babaei-Ghazvini, A., Acharya, B., Badea, I., MacPhee, D. J., & Chen, X.
Affiliations
  • Iran University of Medical Sciences (IR)
  • University of Saskatchewan (CA)
ORCID
Venue
Frontiers in Bioengineering and Biotechnology
Publisher
Frontiers Media SA
Published
2024-10-02
Volume
12
Pages
1418034-1418034
ISSN
2296-4185 (ISSN-L)
DOI
10.3389/fbioe.2024.1418034
PMC
PMC11480021
OpenAlex
W4403081952
Cited by
13
References
1
Citations by year
2026: 7 · 2025: 5 · 2024: 1
FWCI
5.45
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Bioengineering and Biotechnology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Mitacs
  • Natural Sciences and Engineering Research Council of Canada (RGPIN 06369-2019, 2020-05315)
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
Keywords
decellularization, porcine uterus, extracellular matrix, DNA removal, DNase I, peracetic acid, glycosaminoglycans, uterus transplantation, tissue regeneration

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