Genetic and phenotypic assessment of the antimicrobial activity of three potential probiotic lactobacilli against human enteropathogenic bacteria
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Introduction: Lactobacilli are avid producers of antimicrobial compounds responsible for their adaptation and survival in microbe-rich matrices. The bactericidal or bacteriostatic ability of lactic acid bacteria (LAB) can be exploited for the identification of novel antimicrobial compounds to be incorporated in functional foodstuffs or pharmaceutical supplements. In this study, the antimicrobial and antibiofilm properties of Lactiplantibacillus pentosus L33, Lactiplantibacillus plantarum L125 and Lacticaseibacillus paracasei SP5, previously isolated form fermented products, were examined, against clinical isolates of Staphylococcus aureus, Salmonella enterica subsp. enterica serovar Enteritidis and Escherichia coli. Methods: The ability of viable cells to inhibit pathogen colonization on HT-29 cell monolayers, as well as their co-aggregation capacity, were examined utilizing the competitive exclusion assay. The antimicrobial activity of cell-free culture supernatants (CFCS) was determined against planktonic cells and biofilms, using microbiological assays, confocal microscopy, and gene expression analysis of biofilm formation-related genes. Furthermore, in vitro analysis was supplemented with in silico prediction of bacteriocin clusters and of other loci involved in antimicrobial activity. Results: The three lactobacilli were able to limit the viability of planktonic cells of S. aureus and E. coli in suspension. Greater inhibition of biofilm formation was recorded after co-incubation of S. enterica with the CFCS of Lc. paracasei SP5. Predictions based on sequence revealed the ability of strains to produce single or two-peptide Class II bacteriocins, presenting sequence and structural conservation with functional bacteriocins. Discussion: The efficiency of the potentially probiotic bacteria to elicit antimicrobial effects presented a strain- and pathogen-specific pattern. Future studies, utilizing multi-omic approaches, will focus on the structural and functional characterization of molecules involved in the recorded phenotypes.
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Kiousi et al. reported that CFS of Lp. pentosus L33 and Lp. plantarum L125 caused a significant reduction in csgA gene expression in S. enterica.
“These results are consistent with the findings of Kiousi et al.37,, who reported that the CFS of Lp. pentosus L33 and Lp. plantarum L125 caused a significant reduction in the expression of the csgA gene in S. enterica.”
From Probiotic potential of Lactobacillus acidophilus TMPC 41127 isolated from traditional cheese in inhibiting Salmonella Typhi biofilms and virulence genes (2026) · cites this paper as Kiousi et al.37
In Kiousi et al., expression of csgD and cpxR was upregulated, whereas in our research csgD expression was downregulated.
“However, significant differences were observed in the results; for example, in their study, the expression of the csgD and cpxR genes was upregulated, whereas in our research, csgD expression was downregulated.”
From Probiotic potential of Lactobacillus acidophilus TMPC 41127 isolated from traditional cheese in inhibiting Salmonella Typhi biofilms and virulence genes (2026) · cites this paper as Kiousi et al.37
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“These results are consistent with the findings of Kiousi et al. 37 ,, who reported that the CFS of Lp. pentosus L33 and Lp. plantarum L125 caused a significant reduction in the expression of the csgA gene in S. enterica .”
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Prophylactic efficacy of probiotics and their metabolites against Staphylococcus epidermidis
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El-sayed, N. R., Samir, R., Abdel-Hafez, L. J. M., Ibrahim, K. A., Binsuwaidan, R., Selim, H., & Ramadan, M. A. (2026) · Frontiers in Microbiology · doi:10.3389/fmicb.2026.1881796 · Cited by 2 · see citing works
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Low-temperature two-stage probiotic fermentation enhances nutrition and safety of pig liquid feed
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Postbiotics from lactic acid bacteria as promising alternatives against urinary tract pathogens
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- Authors
- Kiousi, D. E., Efstathiou, C., Tzampazlis, V., Plessas, S., Panopoulou, M., Koffa, M., & Galanis, A.
- Affiliations
- Democritus University of Thrace (GR)
- Venue
- Frontiers in Cellular and Infection Microbiology
- Publisher
- Frontiers Media SA
- Published
- 2023-02-08
- Volume
- 13
- Pages
- 1127256-1127256
- ISSN
- 2235-2988 (ISSN-L)
- PMC
- PMC9944596
- OpenAlex
- W4319602549
- Cited by
- 41
- References
- 83
- Citations by year
- 2026: 13 · 2025: 15 · 2024: 10 · 2023: 3
- FWCI
- 5.92
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Cellular and Infection Microbiology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- European Regional Development Fund
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- probiotic lactobacilli, antimicrobial activity, Lactobacillus pentosus, Lactobacillus plantarum, Lactobacillus paracasei, Staphylococcus aureus, Salmonella Enteritidis, Escherichia coli, biofilm inhibition, bacteriocins, class II bacteriocins, bacteriocin gene clusters, competitive exclusion, co-aggregation, cell-free supernatant, fermented foods, functional foods
- MeSH terms
- Anti-Infective Agents, Bacteriocins, Escherichia coli, Humans, Lactobacillaceae, Lactobacillus, Salmonella enteritidis, Staphylococcus aureus, Probiotics
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